Abstract：
:To identify the protein encoded by the L7 region of bovine adenovirus-3 (BAdV-3), specific antisera were raised by immunizing rabbits with bacterial fusion proteins encoding the N-terminus or C-terminus of the BAdV-3 fiber protein. Immunoprecipitation and Western blot analysis confirmed that the fiber is expressed as a 102 kDa glycoprotein, which is localized to the nucleus of infected cells. To identify the nuclear localization signals (NLS), BAdV-3 fiber deletion mutants and GFP/beta-galactosidase fusion proteins were expressed in transfected cells, and subcellular localization was visualized by immunofluorescence microscopy. Analysis of deletion mutants localized the NLS to the N-terminal 41 amino acids. Analysis of the N-terminal 41 amino acids identified a cluster of basic residues between amino acid 14 and 20. Substitution of the basic residues (16KAKR19) with acidic residues (16EAEE19) resulted in the accumulation of fiber in the cytoplasm. However, 16KAKR19 or 12VYPYKAKRPNI22 were not sufficient for efficient transport of a cytoplasmic protein GFP/beta-galactosidase to the nucleus. The recombinant BAdV-3 expressing mutant fiber containing 16EAEE19 instead of 16KAKR19 was unable to replicate efficiently in Madin-Darby bovine kidney cells, suggesting that the NLS of fiber carries out important in vivo functions.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Wu Q,Chen Y,Kulshreshtha V,Tikoo SKdoi
10.1007/s00705-004-0323-xkeywords:
subject
Has Abstractpub_date
2004-09-01 00:00:00pages
1783-99issue
9eissn
0304-8608issn
1432-8798journal_volume
149pub_type
杂志文章abstract：:A regional epidemic of aseptic meningitis caused by echovirus 30 (E30) occurred in Hokkaido, Japan, during the period of August-December 2017. To investigate their phylogenetic relationship to other human enteroviruses, we determined the complete genomic nucleotide sequences of isolates from this outbreak. Phylogeneti...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04484-7
更新日期：2020-02-01 00:00:00
abstract：:An immunofluorescence test on smears of nasal epithelial cells was used to detect coronavirus infection in the respiratory tract of calves. Thirteen gnotobiotic calves were infected with coronavirus isolates derived from faeces or respiratory material: virus was detected in faeces and nasal swabs from all animals. In ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317007
更新日期：1985-01-01 00:00:00
abstract：:Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea and dehydration with high mortality rates in swine. It has become increasingly problematic in China. Since the nucleocapsid (N) protein is highly conserved, it is a candidate protein for early diagnosis and vaccine development. In this study, the N genes of ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1592-4
更新日期：2013-06-01 00:00:00
abstract：:Avian influenza virus infections are a major cause of morbidity and rapid identification of the virus has important clinical, economical and epidemiological implications. We have developed a one-tube Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) for the rapid diagnosis of avian influenza A. A panel of refer...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170193
更新日期：2001-01-01 00:00:00
abstract：:Severe fever with thrombocytopenia syndrome (SFTS) is caused by SFTS virus (SFTSV). Although SFTS originated in China, it is an emerging infectious disease with prevalence confirmed in Japan, Korea, and Vietnam. The full-length genomes of 51 Korean SFTSV isolates from 2013 to 2016 were sequenced, and the sequences wer...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04733-0
更新日期：2020-11-01 00:00:00
abstract：:We discovered a soybean mosaic virus (SMV) variant (4278-1) that caused systemic infections in Nicotiana benthamiana plants, resulting in stem stunting and leaf shriveling. The virus had a particle morphology and incubation period similar to those of other SMV isolates but differed from them in the leaf symptoms it ca...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3123-1
更新日期：2017-02-01 00:00:00
abstract：:Hepatitis C virus (HCV) genome contains a 3'noncoding region (3'NCR) consisting of a variable region, a polypyrimidine tract (polyU/UC) and the X region. To examine the roles of 3'NCR and polyU/UC tract in the internal ribosome entry site (IRES)-mediated translation process, a variety of 3'NCRs containing different le...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0491-3
更新日期：2005-06-01 00:00:00
abstract：:Treatment of a mouse macrophage cell line Mk1 with pokeweed mitogen (PWM) either before or during but not after virus inoculation resulted in an enhancement of dengue virus (DV) infection. The infection enhancement was primarily due to an increase in the number of DV-infected cells but not to increased virus productio...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01379106
更新日期：1994-01-01 00:00:00
abstract：:China experienced an outbreak of equine influenza during 2007-2008. Meanwhile, its neighbor countries, such as Mongolia, India and Japan, have also been affected by various influenza virus strains in each country. Phylogenetic analysis showed that the newly emerging Chinese strains belong to Florida sublineage clade 2...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0724-y
更新日期：2010-09-01 00:00:00
abstract：:The complete genome sequences of isolates of two new bipartite begomoviruses (genus Begomovirus, family Geminiviridae) found infecting common bean in Venezuela are provided. The names proposed for each of these viruses are "bean yellow chlorosis virus" (BYCV) and "bean white chlorosis mosaic virus" (BWCMV). Phylogenet...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1545-y
更新日期：2013-03-01 00:00:00
abstract：:Human respiratory syncytial virus (RSV) is recognized as a leading pathogen responsible for severe respiratory infections in the pediatric population, particularly in infants and young children. A previous study by the same study group revealed that the RSV BA strain was prevalent in southwestern China in epidemic sea...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1552-z
更新日期：2013-07-01 00:00:00
abstract：:Begomovirus infection is becoming a threat in fresh-market tomato in Argentina, where mixed infections with begomoviruses are common. The complete sequence of a begomovirus isolate infecting tomato sampled in Salta was molecularly characterized. Phylogenetic analysis showed that this virus isolate is closely related t...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1355-2
更新日期：2012-10-01 00:00:00
abstract：:BK virus (BKV)-transformed cells and BKV-induced tumors as well as in vitro derived tumor cell lines were all found to contain BKV DNA sequences when analysed by DNA-DNA reassociation kinetics. In transformed cells the number of viral genome equivalents per diploid cell genome (CG divided by VG) decreased with increas...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314838
更新日期：1981-01-01 00:00:00
abstract：:The structural proteins of hemorrhagic enteritis (HEV), a turkey adenovirus, were analyzed by polyacrylamide gel electrophoresis (PAGE) and Western blotting using polyspecific, monospecific and monoclonal antibodies for detection. In purified HEV preparations, eleven polypeptides with apparent molecular weights rangin...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309695
更新日期：1992-01-01 00:00:00
abstract：:A cross-sectional study was undertaken during 2012-2013 to determine the prevalence, strains and factors associated with rotavirus infection among under-5-year-old children hospitalized with acute diarrhea in Uganda. Rotaviruses were detected in 37 % (263/712) of the children. The most prevalent strains were G9P[8] (2...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2742-2
更新日期：2016-04-01 00:00:00
abstract：:The viral reverse transcriptase gene was amplified from the plasma of 27 drug-naïve and five drug-experienced HIV patients in Northern India. Follow-up samples of naïve patients after antiretroviral therapy initiation were collected in six patients at 3 months and three patients at 6 months. Phylogenetic analysis reve...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0605-4
更新日期：2010-04-01 00:00:00
abstract：:The clinical performance of the newly developed MeltPro® HPV Test, based on multicolor melting curve analysis, was evaluated and compared with the commercially available Cobas® HPV Test for detection of HPV and genotyping of HPV-16 and HPV-18. A total of 1647 cervical samples were analyzed with both tests. The agreeme...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3645-1
更新日期：2018-03-01 00:00:00
abstract：:The individual structural polypeptides of HVJ (haemagglutinating virus of Japan--the Sendai strain of parainfluenza 1 virus) were examined by tryptic peptide analysis. [3H]-methionine-labelled structural proteins of the wild-type virus of HVJ (HVJ-W) and [35S]-methionine-labelled corresponding constituent proteins of ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320600
更新日期：1979-01-01 00:00:00
abstract：:Here we determined the relative expression of HERV-K and W proviruses in HIV infected and non-infected mothers as well as their respective babies up to 1 year-old. HIV-infected mothers, their babies and uninfected control groups presented expression of both HERV-K and HERV-W with relatively high frequency. While the l...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3167-2
更新日期：2017-03-01 00:00:00
abstract：:A physical map for the genome of Spodoptera littoralis (Egyptian cottonworm) nuclear polyhedrosis virus of B-type (S1MNPV-B) [Cherry CL, Summers MD (1985) J Invertebr Pathol 46: 289-295] was prepared using the restriction endonucleases SmaI, SfiI, NotI, ApaI, KpnI, BstEII, PstI, SacI, and HindIII. The size of the geno...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313815
更新日期：1989-01-01 00:00:00
abstract：:This study aimed at investigating the effect of progesterone on interferon signaling pathways in peripheral blood mononuclear cells (PBMCs) of patients infected with hepatitis C virus (HCV). PBMCs were isolated from peripheral blood of 38 treatment-naïve HCV-infected patients, pooled, and stimulated with progesterone ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1673-z
更新日期：2013-08-01 00:00:00
abstract：:Two contigs with high similarity to partitivirus sequences were identified by de novo assembly of sequences obtained by RNA-Seq from a wild brassicaceous plant, Arabidopsis halleri subsp. gemmifera. Here, we report the complete genome sequence of a putative novel partitivirus. Excluding the poly-A tail, it consists of...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04670-y
更新日期：2020-09-01 00:00:00
abstract：:TT virus (TTV), the first human circovirus to be discovered, appears to be present in most people; less is known about the prevalence of the related TTV-like mini virus (TLMV). A sensitive nested PCR, specific for TLMV, detected the virus in 48% of 201 sera (Norwegian blood donors) previously found to have a 90% preva...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s705-002-8310-6
更新日期：2002-01-01 00:00:00
abstract：:Here, we present the complete genomic sequence of duck enteritis virus (DEV) strain SD, isolated in China in 2012. The virus was virulent in experimentally infected 2-month-old ducks. The DEV SD genome is 160,945 base pairs (bp) in length. The viral genome sequence, when compared to that of strain DEV CSC, which was i...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04594-7
更新日期：2020-07-01 00:00:00
abstract：:In previous work, we demonstrated that the arenavirus Junín virus (JUNV) is able to activate Akt by means of the phosphatidylinositol-3-kinase (PI3K) survival pathway during virus entry. This work extends our study, emphasizing the relevance of this pathway in the establishment and maintenance of persistent infection ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2298-6
更新日期：2015-02-01 00:00:00
abstract：:The complete nucleotide sequence of genome segment S9, which was reported as the ninth largest among the 12 genome segments of rice dwarf virus, was determined. The segment was 1,305 bp long and coded for a protein composed of 351 amino acids (Mr 38,930). The terminal sequences of the segment were found to consist of ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313885
更新日期：1989-01-01 00:00:00
abstract：:Porcine circovirus type 2 (PCV2) is the essential infectious agent causing porcine circovirus-associated disease (PCVD) in pigs and one of the important viruses that severely jeopardize the swine husbandry industry. PCV2 elicits the unfolded protein response (UPR) via activation of the PERK pathway, and its capsid pro...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04185-1
更新日期：2019-05-01 00:00:00
abstract：:Poliovirus induces bcl-2-independent apoptosis in the human U937 promonocytic cell line [28]. Here we describe that this cell death, induced after viral infection, correlates with the modulation of the protooncoprotein Bcl-xl. Furthermore, poliovirus infection decreases the detected Bcl-xl in a U937 clone that overexp...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0881-8
更新日期：2002-12-01 00:00:00
abstract：:Fecal virus shedding, seroconversion and histopathology were evaluated in 3-7-year-old gnotobiotic calves orally inoculated with porcine deltacoronavirus (PDCoV) (9.0-9.6 log10 genomic equivalents [GE] of OH-FD22-P5; n=4) or porcine epidemic diarrhea virus (PEDV) (10.2-12.5 log10 GE of PC21A; n=3). In PDCoV-inoculated...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3351-z
更新日期：2017-08-01 00:00:00
abstract：:Glycoprotein D (gD) of bovine herpesvirus 1 (BHV-1), a homolog of herpes simplex virus gD, represents a major component of the viral envelope and is a dominant immunogen. To study the antigenic properties of the different regions of gD, we have expressed the full-length gD encoding gene and overlapping fragments spann...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050450
更新日期：1998-01-01 00:00:00
