Abstract：
:Glycoprotein D (gD) of bovine herpesvirus 1 (BHV-1), a homolog of herpes simplex virus gD, represents a major component of the viral envelope and is a dominant immunogen. To study the antigenic properties of the different regions of gD, we have expressed the full-length gD encoding gene and overlapping fragments spanning various regions of the gD open reading frame in a baculovirus (Autographa californica nuclear polyhedrosis virus)--insect cell (Spodoptera frugiperda, SF-9) system. Maximum levels of expression for all proteins were obtained 48 to 72 h post infection of SF-9 cells by recombinant viruses. Full-length and truncated recombinant gD proteins reacted specifically with anti-gD monospecific serum as determined by immunoprecipitation and immunoblotting, indicating that the proteins retained their antigenicity. However, based on the reactivity with a panel of gD-specific monoclonal antibodies (Mabs), the full-length recombinant gD lacked proper expression for two highly neutralizing linear epitopes identified by Mabs R54 and 9D6. The rest of the epitopes appeared to be preserved and antigenically unaltered. Immunofluorescence studies of recombinant baculovirus infected SF-9 cells using gD monospecific serum, revealed no direct correlation between cellular localization of the expressed proteins and their amino acid sequences.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Abdelmagid OY,Mansour MM,Okwumabua O,van Drunen Littel-van den Hurk Sdoi
10.1007/s007050050450subject
Has Abstractpub_date
1998-01-01 00:00:00pages
2173-87issue
11eissn
0304-8608issn
1432-8798journal_volume
143pub_type
杂志文章abstract：:Genetic relationships among echovirus type 25 (ECV 25) isolates associated with aseptic meningitis in Germany 1997/98 and a 40-year-old ECV 25 prototype strain were investigated using RT-PCR and direct sequencing the VP1/2A gene junction region. Sequences were compared to each other and to non-polio enterovirus repres...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050640
更新日期：1999-01-01 00:00:00
abstract：:Tri (n-butyl) phosphate (TNBP)-split vaccine containing 6400 CCA units of influenza virus A/Aichi/68 (H3N2) was given intramuscularly to a group of volunteers. The changes in serum haemagglutination-inhibiting (HI) and nasal wash neutralizing antibody were measured, and the results compared with that of volunteers giv...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320555
更新日期：1975-01-01 00:00:00
abstract：:Using a one-step model for cell-to-cell transmission of HIV infection we have identified two distinct phases of HIV RNA synthesis. The first phase (4 h-12 h p.i.) was marked by an increase in only the full-length 9 kb RNA, while the second phase (24 h p.i. onwards) comprised a significant increase in the levels of all...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050429
更新日期：1998-01-01 00:00:00
abstract：:We studied 67 hepatitis C virus (HCV) isolates from 64 hospitalized patients in Shanghai, China. Genotype 1 was prevalent, and genotypes 2, 3, 6 were found for the first time in Shanghai. A rare mixed infection with three subtypes (1a, 1b, 2a) was found. The complete genome sequence of a subtype 3b isolate was determi...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1457-x
更新日期：2013-01-01 00:00:00
abstract：:The alteration of whole Sendai virus and especially of its nucleocapsid polypeptides, during storage of the virus at 4 degree C in the allantoic fluids in which it was cultivated, has cultivated, has been studied by sodium dodecyl sulfate gel electrophoresis. During virus storage the nucleocapsid protein subunits with...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317427
更新日期：1975-01-01 00:00:00
abstract：:The numbers of the carbohydrate side chains have been analyzed on a series of subtype 7 hemagglutinins of influenza A virus. The procedure employed involves growth of the virus in the presence of the trimming inhibitor N-methyl-1-desoxynojirimycin followed by controlled removal of the resulting mannose-rich oligosacch...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314433
更新日期：1987-01-01 00:00:00
abstract：:The complete genome sequence of grapevine Bulgarian latent virus (GBLV) has been determined. RNA-1 (7,452 nt in length) contains a single ORF of 6,285 nt, encoding a polyprotein with conserved motifs characteristic of the viral protease cofactor (Prot-cofact), the NTP-binding protein (NTP), the cysteine-like protease ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0908-5
更新日期：2011-05-01 00:00:00
abstract：:The genomic RNA sequences of two genotypes of a brassica-infecting polerovirus from China were determined. Sequence analysis revealed that the virus was closely related to but significantly different from turnip yellows virus (TuYV). This virus and other poleroviruses, including TuYV, had less than 90% amino acid sequ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1091-z
更新日期：2011-12-01 00:00:00
abstract：:The synthesis of M protein of HVJ (Sendai virus) in rat glioma (C6) cells, as has previously been reported, is selectively reduced at a nonpermissive temperature of 39 degrees C. In this phenomenon no difference was found in the viral RNA synthesis and the stability of the viral RNAs between the permissive and nonperm...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310830
更新日期：1988-01-01 00:00:00
abstract：:Although winter dysentery (WD), which is caused by the bovine coronavirus (BCoV) is characterized by the sudden onset of diarrhea in many adult cattle in a herd, the pathogenesis of the WD-BCoV is not completely understood. In this study, colostrum-deprived calves were experimentally infected with a Korean WD-BCoV str...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-1005-2
更新日期：2007-01-01 00:00:00
abstract：:Molecular variability was assessed for 18 isolates of cowpea mild mottle virus (CPMMV, genus Carlavirus, family Betaflexiviridae) found infecting soybean in various Brazilian states (Bahia, Goiás, Maranhão, Mato Grosso, Minas Gerais, Pará) in 2001 and 2010. A variety of symptoms was expressed in soybean cv. CD206, ran...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1879-0
更新日期：2014-04-01 00:00:00
abstract：:B-cell epitopes of varicella-zoster virus glycoprotein II were mapped by means of solid phase ELISA, synthetic oligopeptides (constructed according to the Davison-Scott sequencing of the varicella-zoster virus genome) and sera from varicellae and herpes zoster patients. The individual pattern of antibody peptide bindi...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718644
更新日期：1996-01-01 00:00:00
abstract：:Our previous studies demonstrated that hepatitis E virus (HEV) requires the multivesicular body (MVB) pathway to release virus particles, suggesting that HEV utilizes the cellular ESCRT machinery in the cytoplasm, not at the cell surface, to be released from infected cells. In this study, we generated a murine monoclo...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1912-3
更新日期：2014-05-01 00:00:00
abstract：:The molecular epidemiology of a recent norovirus (NoV) outbreak in Brazil performed by comparative analysis with Genebank NoV sequences showed that the GII.4 strain was responsible for 72.5% of all NoV-positive cases (58/80). Other detected NoV strains included GII.3 (7/80; 8.8%) and GII.9 (8/80; 10%). This is the fir...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0078-x
更新日期：2008-01-01 00:00:00
abstract：:The betaherpesvirus human herpesvirus 6 (HHV-6) has two variants. The U83 gene product of strain HST is a chemoattractant for monocytes. Here, we describe U83 gene variations that accumulated in variants A and B. A gene-variation hot spot was examined in 36 different strains and one donor DNA sample. U83 gene variatio...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0307-3
更新日期：2009-01-01 00:00:00
abstract：:To provide information on the mechanism of attenuation of a Newcastle disease vaccine strain, TCND, we compared it with the parental virulent strain California 11,914 (CAL) biologically and genetically. It was found that TCND bore the fusion protein of virulent type, consisting of a pair of dibasic amino acid residues...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050361
更新日期：1998-01-01 00:00:00
abstract：:The ICP34.5 gene from HSV-2 strain 333 was cloned and, when expressed in Vero cells, enhanced the efficiency and extent of glycoprotein processing of glycoprotein C (gC1), a representative viral glycoprotein, during infection with HSV-1 SP7. The ICP34.5 from HSV-1 SP7 limits the extent and efficiency of viral glycopro...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0341-9
更新日期：2009-01-01 00:00:00
abstract：:The structure of the scrapie agent remains unknown. However, scrapie infectivity tends to co-sediment with an infection specific fraction of the glycoprotein PrP (PrPSc) under conditions which solubilise the normal form of this protein (PrPc); accordingly, PrP has been proposed as a candidate component of the agent. T...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718399
更新日期：1996-01-01 00:00:00
abstract：:The clinical performance of the newly developed MeltPro® HPV Test, based on multicolor melting curve analysis, was evaluated and compared with the commercially available Cobas® HPV Test for detection of HPV and genotyping of HPV-16 and HPV-18. A total of 1647 cervical samples were analyzed with both tests. The agreeme...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3645-1
更新日期：2018-03-01 00:00:00
abstract：:A large sheep flock screened over a two-year period showed active spreading of Schmallenberg virus (SBV) during the summers of 2011 and 2012. Transplacental infections were observed during the two associated lambing periods (the winters of 2012 and 2013). Analysis of small (S) segment sequences of 38 SBV-positive samp...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2787-x
更新日期：2016-05-01 00:00:00
abstract：:A novel bisegmented double-stranded RNA virus was identified in the mycoparasitic and opportunistic fungus Hypomyces chrysospermus. The RNA1 genome segment comprises 1866 bp and encodes an RNA-dependent RNA polymerase (RdRp). The RNA2 segment comprises 1822 bp and encodes a capsid protein. Phylogenetic analysis of the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04359-x
更新日期：2019-10-01 00:00:00
abstract：:Murine monoclonal antibodies against the fusion (F) and hemagglutinin-neuraminidase (HN) proteins of Sendai virus (SV) were prepared and studied on their antiviral activities, particularly on the neutralization of infectivity. On the analysis with solid phase competitive ELISA, 26 anti-HN antibodies were divided into ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01316735
更新日期：1986-01-01 00:00:00
abstract：:TT virus (TTV), the first human circovirus to be discovered, appears to be present in most people; less is known about the prevalence of the related TTV-like mini virus (TLMV). A sensitive nested PCR, specific for TLMV, detected the virus in 48% of 201 sera (Norwegian blood donors) previously found to have a 90% preva...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s705-002-8310-6
更新日期：2002-01-01 00:00:00
abstract：:In order to investigate the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) strains currently circulating in the Republic of Ireland (ROI), the ORF5 gene from 17 field strains originating from four vaccinating commercial herds was sequenced and phylogenetically analysed. High genetic v...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04710-7
更新日期：2020-09-01 00:00:00
abstract：:Astroviruses are associated with acute gastroenteritis of human and many animal species. Recently, two studies have reported that novel astroviruses were found in bats. In order to extensively understand the genetic and phylogenetic characterization of bat astroviruses, we tested fecal samples of nine bat species that...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1011-2
更新日期：2011-08-01 00:00:00
abstract：:A putative chrysovirus recovered from Brassica campestris var. purpurea and provisionally named "Brassica campestris chrysovirus 1" (BrcCV1) was sequenced. The genome of the putative BrcCV1 consists of three double-stranded RNAs (dsRNAs) comprising 3,639 (dsRNA 1), 3,567 (dsRNA 2) and 3,337 (dsRNA 3) base pairs, respe...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3196-x
更新日期：2017-04-01 00:00:00
abstract：:This study aimed at investigating the effect of progesterone on interferon signaling pathways in peripheral blood mononuclear cells (PBMCs) of patients infected with hepatitis C virus (HCV). PBMCs were isolated from peripheral blood of 38 treatment-naïve HCV-infected patients, pooled, and stimulated with progesterone ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1673-z
更新日期：2013-08-01 00:00:00
abstract：:The RNAs of 4 avian rotavirus isolates were analysed by polyacrylamide gel electrophoresis. One of these isolates was made from chickens, the other three were from turkeys. The RNAs were separated into 8 or 9 bands consisting of 11 segments, ranging in molecular weight from 0.24 X 10(6) to 2.09 X 10(6). In contrast to...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320765
更新日期：1980-01-01 00:00:00
abstract：:The aim of this study was to evaluate the long-term effectiveness of first-line antiretroviral therapy in HIV/AIDS patients in Southeast China. A total of 450 eligible patients were selected to initiate first-line antiretroviral therapy from February 2005 through August 2009. During the study period from 2009 through ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2583-z
更新日期：2015-11-01 00:00:00
abstract：:The sequence variability of Barley yellow dwarf virus-PAV (PAV) and Barley yellow dwarf virus-MAV (MAV) was studied by comparing 502 nucleotides from the coat protein-encoding region of six isolates from Latvia and four from Sweden. The diversity within MAV was low (>97% sequence identity), also when compared to isola...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0242-2
更新日期：2004-04-01 00:00:00
