Abstract：
:Using a one-step model for cell-to-cell transmission of HIV infection we have identified two distinct phases of HIV RNA synthesis. The first phase (4 h-12 h p.i.) was marked by an increase in only the full-length 9 kb RNA, while the second phase (24 h p.i. onwards) comprised a significant increase in the levels of all three species of viral RNA. We now report that while the continual presence of actinomycin D at 50 micrograms/ml abolished all detectable viral nucleic acid synthesis when virus donor H3B cells were pre-treated with 50 micrograms/ml of actinomycin D (AmD), washed free of unbound drug (a procedure which inhibited > 99% of total cellular RNA transcription) then mixed with untreated recipient Hut 78 cells, normal amounts of full length linear unintegrated viral DNA were produced and the first phase of viral RNA transcription was unaffected. Similarly, when both the virus donor cells and recipient cells were arrested in the late G1 phase of the cell cycle by aphidicolin and then mixed, linear unintegrated viral DNA was the major viral DNA species produced. The appearance of circular viral DNA and progeny virus was inhibited, but the first phase of induced viral RNA synthesis was unaffected. When AZT was added at 2 h or 4 h after cell-cell mixing, the level of 9 kb RNA detected was significantly lower, corresponding to reduction in the level of viral DNA. These and previous results indicate that the template for the first phase of viral RNA synthesis was likely to be newly synthesized, linear unintegrated viral DNA and not pre-existing proviral DNA present in the donor cells. Taken together, our results suggest that there exists a yet to be fully characterized pathway of concurrent viral DNA and RNA synthesis early after cell to cell transmission of HIV infection.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Kok TW,Li P,Burrell CJdoi
10.1007/s007050050429subject
Has Abstractpub_date
1998-01-01 00:00:00pages
1911-26issue
10eissn
0304-8608issn
1432-8798journal_volume
143pub_type
杂志文章abstract：:Human bocavirus (HBoV) has been detected primarily in children with acute lower respiratory tract disease (LRTD), but its occurrence, clinical profile, and role as a causative agent of RTD are not clear. The aim of this study was to investigate the prevalence and the potential clinical relevance of HBoV. Using molecul...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04396-6
更新日期：2019-12-01 00:00:00
abstract：:In order to investigate the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) strains currently circulating in the Republic of Ireland (ROI), the ORF5 gene from 17 field strains originating from four vaccinating commercial herds was sequenced and phylogenetically analysed. High genetic v...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04710-7
更新日期：2020-09-01 00:00:00
abstract：:The complete genome sequence of a severe isolate of broad bean true mosaic virus (genus Comovirus, subfamily Comovirinae, family Secoviridae) is presented. Comparison of the amino acid sequences of the capsid proteins and the polymerase showed striking differences to other comoviruses and highest similarities to legum...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0668-2
更新日期：2010-07-01 00:00:00
abstract：:A 945 nucleotide region (bases 76-1020) of the HA1 part of the HA gene was obtained for 31 influenza viruses of H7 subtype isolated primarily from Europe, Asia and Australia over the last 20 years. These were analysed phylogenetically and compared with sequences of the same region from 23 H7 subtype viruses available ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050695
更新日期：2000-01-01 00:00:00
abstract：:The complete genomic sequence of Cassava Ivorian bacilliform virus (CIBV) is described. The virus has a genomic organization similar to that of pelargonium zonate spot virus (PZSV), the type member of the genus Anulavirus, but it is most closely related to a second, recently described, anulavirus, Amazon lily mild mot...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2086-3
更新日期：2014-10-01 00:00:00
abstract：:We previously reported the VP4 and the VP7 genotypes of the first G6P[14] rotavirus strain (RVA/Human-wt/GHA/M0084/2010/G6P[14]) from the stool of an infant with diarrhoea in Ghana. In the current study, we obtained the complete genome sequences using Illumina MiSeq next-generation sequencing to enable us to determine...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04543-4
更新日期：2020-04-01 00:00:00
abstract：:Chilo iridescent virus (CIV) belongs to the family Iridoviridae, which are icosahedral cytoplasmic DNA viruses with large, linear, and circularly permuted genomes. Previous studies on infected-cell-specific polypeptides suggested temporal regulation of CIV gene expression. Recently, we demonstrated three temporal clas...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0232-4
更新日期：2004-04-01 00:00:00
abstract：:It still needs to be elucidated whether co-detection of EV71 with other intestinal tract viruses plays a role in the pathogenesis of severe hand-foot-mouth disease (HFMD). A total of 680 stool specimens collected from clinically diagnosed mild and severe-HFMD patients were tested for EV71, CA16, norovirus, bocavirus a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1396-6
更新日期：2012-11-01 00:00:00
abstract：:A range of isolates of Pea seed-borne mosaic virus (PSbMV) was compared in the segments of the genome representing the partial NIb/CP/UTR and the partial P1-Pro/HC-Pro coding regions. Nucleotide and amino acid sequences, and a phylogenetic analysis of the CP region, divided isolates with available sequence information...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170038
更新日期：2001-10-01 00:00:00
abstract：:A flow cytometric virus binding assay that directly visualizes the binding of infectious bursal disease virus (IBDV) to its target cells was established. The chicken B lymphoblastoid cell line, LSCC-BK3, which is permissive for IBDV infection, bound high levels of the virus. Another B lymphoblastoid cell line, LSCC-11...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050464
更新日期：1998-01-01 00:00:00
abstract：:Tobacco vein banding mosaic virus (TVBMV) is of increasing importance in tobacco production. Knowledge of the genetic structure and variability of the virus population is vital for developing sustainable management. In this study, 24 new TVBMV isolates from Sichuan Province together with 46 previous isolates were stud...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04342-6
更新日期：2019-10-01 00:00:00
abstract：:A novel myovirus, vB_PagM_AAM22 (AAM22), was isolated in Lithuania using Pantoea agglomerans as the host for phage propagation. The 49,744-bp genome of AAM22 has a G + C content of 48.4% and contains 96 probable protein-encoding genes and no genes for tRNA. In total, 34 ORFs were given a putative functional annotation...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04705-4
更新日期：2020-09-01 00:00:00
abstract：:Porcine reproductive and respiratory syndrome (PRRS) is one of the most highly infectious diseases in the pig industry, resulting in enormous economic losses worldwide. In this study, a PRRS virus (PRRSV) strain was isolated from primary porcine alveolar macrophage cells in Xinjiang in northwest China. This new strain...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04373-z
更新日期：2019-11-01 00:00:00
abstract：:Changes in the NP gene of H5N1 highly pathogenic avian influenza (HPAI) viruses have previously been shown to affect viral replication, alter host gene expression levels and affect mean death times in infected chickens. Five amino acids at positions 22, 184, 400, 406, and 423 were different between the two recombinant...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0399-4
更新日期：2009-01-01 00:00:00
abstract：:A new human endogenous retroviral family (HERV-F) has recently been identified from human chromosome 7q31.1-q31.3 that was identical to the XA34 cDNA clone isolated from a human glioma cDNA library with an ERV-9 env probe. We investigated pol fragments of the HERV-F family from the Old World monkeys (crab-eating monke...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s705-002-8327-7
更新日期：2002-01-01 00:00:00
abstract：:Human peripheral blood mononuclear cells (PBMC) can be infected in vitro with sera of hepatitis C virus (HCV)-infected patients. We have treated pools of PBMC with dextran sulfate MW 8,000, before infection with various HCV(+) sera. When the cells were treated with dextran sulfate 10-3 M, HCV RNA was no longer detecta...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050293
更新日期：1998-01-01 00:00:00
abstract：:Mouse cell lines (L, NIH3T3, and RMA cells) infected with the Edmonston strain of measles virus (MV) did not exhibit cytopathic effects (CPE), consistent with the finding that mice are not susceptible to MV. Northern blot analysis, however, revealed that MV genes were transcribed in infected L and NIH3T3 cells, althou...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310037
更新日期：1994-01-01 00:00:00
abstract：:Ninety four percent of the genome of bovine herpesvirus 4 (BHV-4) strain DN 599 was cloned and a physical map was constructed by Southern blot analysis using a library of cloned fragments cleaved with the 3 restriction enzymes (Eco RI, Bam HI, and Hin dIII). The genome length was estimated to be 156.5 kbp +/- 0.7. The...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309576
更新日期：1992-01-01 00:00:00
abstract：:A virus isolated from sorghum in Nigeria has been partially characterized. It was tested by enzyme-linked immunosorbent assay (ELISA) using antisera to Maize dwarf mosaic virus, Johnsongrass mosaic virus (JGMV), Sugarcane mosaic virus strain-MDB, Sorghum mosaic virus, and Zea mosaic virus. A partial host range, sympto...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0411-y
更新日期：2005-03-01 00:00:00
abstract：:Function of rotavirus NSP1 was analyzed by using single-NSP1 gene-substitution reassortants, SKF, SDF, and SNF which have the NSP1 gene derived from human rotaviruses KU, DS-1, and canine rotavirus K9, respectively, in the genetic background of simian rotavirus SA11. The NSP1 genes from KU, DS-1, K9, and SA11 exhibite...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050600
更新日期：1999-01-01 00:00:00
abstract：:Many distinct polyomaviruses infecting a variety of vertebrate hosts have recently been discovered, and their complete genome sequence could often be determined. To accommodate this fast-growing diversity, the International Committee on Taxonomy of Viruses (ICTV) Polyomaviridae Study Group designed a host- and sequenc...
journal_title：Archives of virology
pub_type： 新闻
doi：10.1007/s00705-016-2794-y
更新日期：2016-06-01 00:00:00
abstract：:The entry of enveloped viruses into host cells requires the fusion of viral and cell membranes. These membrane fusion reactions are mediated by virus-encoded glycoproteins. In the case of avian metapneumovirus (aMPV), the fusion (F) protein alone can mediate virus entry and induce syncytium formation in vitro. To inve...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2524-x
更新日期：2015-10-01 00:00:00
abstract：:Norovirus (NoV) infection is the most common cause of nonbacterial acute gastroenteritis, which affects both adults and children. However, the molecular epidemiology of NoV in adults with acute gastroenteritis in China has not been investigated extensively. In this study, we investigated the occurrence of NoV infectio...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2440-0
更新日期：2015-07-01 00:00:00
abstract：:A novel iflavirus, tentatively named "Sogatella furcifera honeydew virus 1" (SFHV1), discovered through transcriptome sequencing analysis of white-backed planthoppers (Sogatella furcifera) collected in southern China, is described here. The full genome of SFHV1 is 10,837 nucleotides (nt) long, including the polyA tail...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3817-7
更新日期：2018-07-01 00:00:00
abstract：:Northern pike from several locations in central Canada were observed to have two different types of hyperplastic epidermal lesions on the body and fins. One type of epidermal hyperplasia consisted of flat lesions, bluish-white in color with a granular or "gritty" appearance. Histological examination of this granular l...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310815
更新日期：1984-01-01 00:00:00
abstract：:Hepatitis C virus (HCV) genome contains a 3'noncoding region (3'NCR) consisting of a variable region, a polypyrimidine tract (polyU/UC) and the X region. To examine the roles of 3'NCR and polyU/UC tract in the internal ribosome entry site (IRES)-mediated translation process, a variety of 3'NCRs containing different le...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0491-3
更新日期：2005-06-01 00:00:00
abstract：:Sequence analysis of a 6.4 kb DNA region from the Spodoptera littoralis multinucleocapsid nucleopolyhedrovirus (SpliNPV) revealed a large open reading frame (ORF) encoding a predicted polypeptide of 998 amino acid (aa) residues with a molecular mass of 114.93 kDa, located between 47.2-52.3 m.u. on the SpliNPV genome. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170177
更新日期：2001-01-01 00:00:00
abstract：:The present study reports the first complete genome sequence analysis of West Nile virus (WNV) strains isolated from brain samples from raptors. The results prove the circulation of closely related WNV lineage II strains in central Europe and genetic analysis revealed seven amino acid substitutions in structural (PrM3...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-4056-7
更新日期：2019-01-01 00:00:00
abstract：:The distribution of African swine fever virus (ASFV) in whole blood, plasma, red blood cells (RBC) and white blood cell (WbC) sub-populations was determined in pigs infected with virulent virus. Changes in the RBC and WBC populations were also examined. Total WBC counts decreased and RBC numbers remained unchanged dur...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315054
更新日期：1977-01-01 00:00:00
abstract：:Tri (n-butyl) phosphate (TNBP)-split vaccine containing 6400 CCA units of influenza virus A/Aichi/68 (H3N2) was given intramuscularly to a group of volunteers. The changes in serum haemagglutination-inhibiting (HI) and nasal wash neutralizing antibody were measured, and the results compared with that of volunteers giv...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320555
更新日期：1975-01-01 00:00:00
