Abstract:
:A virus isolated from sorghum in Nigeria has been partially characterized. It was tested by enzyme-linked immunosorbent assay (ELISA) using antisera to Maize dwarf mosaic virus, Johnsongrass mosaic virus (JGMV), Sugarcane mosaic virus strain-MDB, Sorghum mosaic virus, and Zea mosaic virus. A partial host range, symptom phenotypes for selected sorghum lines, and the mass of the coat protein (CP) subunit was analyzed by sodium-dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and its amino acid (aa) sequence determined by time-of-flight mass spectrometry (TOFMS). The Nigerian isolate was positive in ELISA to only JGMV antiserum. It infected sorghum and smooth brome but not oat or johnsongrass. It caused necrosis in 12 of 13 tested sorghum lines, while the USA JGMV isolate caused necrosis in only one sorghum line. In SDS-PAGE, the mass of the Nigerian virus CP was 3,000 Da smaller than that of JGMV-MDO. Moreover, TOFMS analyses showed that, while residues 1-7 of the CP aa sequence were identical to those of JGMV (GenBank #A27631), and residues 57-293 were almost identical to residues 67-303 of JGMV, the intermediate region exhibited significant differences, including a 10 aa deletion. These data indicate that the virus should be considered a distinct isolate of JGMV (JGMV-N) and expands the known range of JGMV to Africa.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Seifers DL,Haber S,Ens W,She YM,Standing KG,Salomon Rdoi
10.1007/s00705-004-0411-ykeywords:
subject
Has Abstractpub_date
2005-03-01 00:00:00pages
557-76issue
3eissn
0304-8608issn
1432-8798journal_volume
150pub_type
杂志文章abstract::117F and R are subgroup I and II cucumber mosaic virus (CMV) strains, respectively. Whereas I17F induces severe symptoms on all hosts so far tested, R induces generally mild symptoms, except on Nicotiana glutinosa, on which it causes leaf blistering and severe stunting. Pseudorecombinants and recombinants, based on RN...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050510
更新日期:1999-01-01 00:00:00
abstract::We have developed an in vitro transcriptional assay using Escherichia coli RNA polymerase to initiate the replication of peach latent mosaic viroid (PLMVd). Regardless of the polarity of the PLMVd strand used as template, initiation in vitro occurred at the same hairpin structure. These initiation sites correspond to ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050170061
更新日期:2001-01-01 00:00:00
abstract::Non-neoplastic tonsils were analyzed for detection of Epstein-Barr virus (EBV)-positive cells by in situ hybridization and in situ PCR. EBV-encoded small nuclear RNA 1(EBER1)-positive cells were found in 28.2% of the tonsils and were evenly localized in the extrafollicular area and within germinal centers. Latent memb...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050332
更新日期:1998-01-01 00:00:00
abstract::To investigate the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) in Northern Ireland, the ORF5 gene from nine field isolates was sequenced and phylogenetically analysed. The results revealed relatively high diversity amongst isolates, with 87.6-92.2% identity between farms at the nuc...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3886-7
更新日期:2018-10-01 00:00:00
abstract::A novel bisegmented double-stranded RNA virus was identified in the mycoparasitic and opportunistic fungus Hypomyces chrysospermus. The RNA1 genome segment comprises 1866 bp and encodes an RNA-dependent RNA polymerase (RdRp). The RNA2 segment comprises 1822 bp and encodes a capsid protein. Phylogenetic analysis of the...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04359-x
更新日期:2019-10-01 00:00:00
abstract::Two cases of coinfection with bovine papular stomatitis virus (BPSV) and pseudocowpox virus (PCPV) in dairy calves in Tochigi Prefecture, Japan, are reported. Sequences of BPSV and PCPV were simultaneously detected in the same polymerase chain reaction (PCR) amplicons, which were obtained from the DNA of two dairy cal...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04792-3
更新日期:2020-11-01 00:00:00
abstract::Mycobacteriophage archival stocks have been kept for ca. 20-50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreov...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3788-8
更新日期:2018-07-01 00:00:00
abstract::The effect of temperature on the response of tomato (Lycopersicon esculentum Mill. cv. Rutgers) to infections with the necrogenic cucumber mosaic virus (CMV) satellite D-CARNA 5 was investigated with each of four CMV strains D, 1, Y and S functioning as helper virus. At 24 degrees C lethal necrosis was observed in all...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309724
更新日期:1995-01-01 00:00:00
abstract::A 945 nucleotide region (bases 76-1020) of the HA1 part of the HA gene was obtained for 31 influenza viruses of H7 subtype isolated primarily from Europe, Asia and Australia over the last 20 years. These were analysed phylogenetically and compared with sequences of the same region from 23 H7 subtype viruses available ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050695
更新日期:2000-01-01 00:00:00
abstract::Truncated alpha 4 genes were introduced into BHK tk- cells along with the neomycin phosphotransferase gene, that confers resistance to the eukaryotic antibiotic G418, driven by the HSV-1 beta tk promoter (beta tk- neor). Stably transformed cell lines were obtained and studied for the ability of the resident truncated ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01316907
更新日期:1993-01-01 00:00:00
abstract::Work from a number of laboratories including our own has shown that foreign B-epitopes inserted into the c/e1-region of Hepatitis B core antigen (HBcAg) elicit powerful antibody responses when mice are immunised with the recombinant core particles. In the present study, we wished to take advantage of the immunodominan...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050590
更新日期:1999-01-01 00:00:00
abstract::Amino acid positions recognized by monoclonal antibodies (MAbs) in the influenza A virus nucleoprotein (NP) have been reported. As these residues were scattered in the three-dimensional (3D) structure of NP, no patterns of the architecture of antibody-binding sites could be inferred. Here, we used site-specific mutage...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1952-8
更新日期:2014-06-01 00:00:00
abstract::Bacteriophage JBP901, isolated from fermented food, is specific for Bacillus cereus group species and exhibits a broad host spectrum among a large number of B. cereus isolates. Genome sequence analysis revealed a linear 159,492-bp genome with overall G+C content of 39.7 mol%, and 201 ORFs. The presence of a putative m...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2485-0
更新日期:2015-09-01 00:00:00
abstract::Serologic and virologic prevalence of infection with different swine influenza virus (SIV) subtypes was investigated using swine sera, nasal swabs and lung samples that had been submitted for a diagnosis to the Minnesota Veterinary Diagnostic Laboratory. A total of 111,418 pig sera were tested for SIV antibody between...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-002-0788-4
更新日期:2002-06-01 00:00:00
abstract::Arginine is necessary for the development of the cytopathogenic effect of human cytomegalovirus in human embryonic fibroblasts. It is also required, though in greater concentrations, for the production of infective virions, the requirement being at an early stage of replication. Inhibitor studies suggested that this b...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01318146
更新日期:1975-01-01 00:00:00
abstract::Coxsackievirus B3 (CV-B3) has frequently been associated with aseptic meningitis outbreaks in China. To identify sequence motifs related to aseptic meningitis and to construct an infectious clone, the genome sequence of 08TC170, a representative strain isolated from cerebrospinal fluid (CSF) samples from an outbreak i...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2893-9
更新日期:2016-08-01 00:00:00
abstract::Despite reports that the PB1-F2 protein contributes to influenza virus pathogenicity in the mouse model, little is known about its significance in avian hosts. In our previous study, the A/Vietnam/1203/04 (H5N1) wild-type virus (wtVN1203) was more lethal to mallard ducks than a reverse genetics (rg)-derived VN1203. In...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0666-4
更新日期:2010-06-01 00:00:00
abstract::Samples containing two viruses belonging to the genus Polerovirus, beet chlorosis virus (BChV) and beet mild yellowing virus (BMYV), were collected from French and Polish sugar beet fields. The molecular properties of 24 isolates of BChV and BMYV were investigated, and their genetic diversity was examined in the coat ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2245-6
更新日期:2015-02-01 00:00:00
abstract::In order to investigate the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) strains currently circulating in the Republic of Ireland (ROI), the ORF5 gene from 17 field strains originating from four vaccinating commercial herds was sequenced and phylogenetically analysed. High genetic v...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04710-7
更新日期:2020-09-01 00:00:00
abstract::B-cell epitopes of varicella-zoster virus glycoprotein II were mapped by means of solid phase ELISA, synthetic oligopeptides (constructed according to the Davison-Scott sequencing of the varicella-zoster virus genome) and sera from varicellae and herpes zoster patients. The individual pattern of antibody peptide bindi...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718644
更新日期:1996-01-01 00:00:00
abstract::The complete genome sequence of a severe isolate of broad bean true mosaic virus (genus Comovirus, subfamily Comovirinae, family Secoviridae) is presented. Comparison of the amino acid sequences of the capsid proteins and the polymerase showed striking differences to other comoviruses and highest similarities to legum...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0668-2
更新日期:2010-07-01 00:00:00
abstract::The plaques formed by wild-type (WT) populations of strain K485 visna (V-K485) virus, strain K796 visna (V-K796) virus, and of progressive pneumonia virus in sheep choroid plexus (SCP) cell cultures are heterogeneous in size. Plaque purification procedures showed that this heterogeneity was due to the presence of two ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01318133
更新日期:1981-01-01 00:00:00
abstract::Avian influenza due to highly pathogenic avian influenza (HPAIV) H5N1 virus is not a food-borne illness but a serious panzootic disease with the potential to be pandemic. In this study, broiler chickens were vaccinated with commercial H5N1 or H5N2 inactivated vaccines prior to being challenged with an HPAIV H5N1 (clad...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1242-x
更新日期:2012-05-01 00:00:00
abstract::Genetic analysis of the complete haemagglutinin (HA) gene of fourteen Nigerian avian influenza isolates showed multiple basic amino acids at the cleavage site (321PQRERRRK del R*GLF333), characteristic of highly pathogenic avian influenza (HPAI). Substitution of Gln to Lys at position 322 (H5-specific numbering) was i...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-3149-4
更新日期:2017-03-01 00:00:00
abstract::The swine pathogen porcine circovirus type 2 (PCV2) causes significant economic damage worldwide. The PCV2 capsid (CP) residues 169-STIDYFQPNNKR-180 have been identified as a decoy epitope that diverts the host immune response away from protective epitopes. However, the decoy epitope may include important linear or co...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04831-z
更新日期:2020-12-01 00:00:00
abstract::The complete genome of O/Akesu/58 strain of foot-and-mouth disease virus (FMDV) was sequenced. The phylogenetic analysis revealed that it is not closely related to epidemic strains or previous strains compared with reference sequences (the identities of complete VP1 nucleotide sequences range from 77.5 to 84.0%). Its ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-007-1041-y
更新日期:2007-01-01 00:00:00
abstract::In this work, we describe the complete sequence and genome organization of a novel tobamovirus detected in a prickly pear plant (Opuntia sp.) by high-throughput sequencing, tentatively named "opuntia virus 2". The full genome of opuntia virus 2 is 6,453 nucleotides in length and contains four open reading frames (ORFs...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04528-3
更新日期:2020-03-01 00:00:00
abstract::Bokeloh bat lyssavirus (BBLV) was found in Myotis nattereri for the first time in northeastern France in July 2012. The complete genome sequence of the virus from the infected Natterer's bat was determined by whole-genome sequencing and compared to that of the first BBLV strain isolated in 2010 in Germany and with tho...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1747-y
更新日期:2013-11-01 00:00:00
abstract::Lymphocytic chorimeningitis virus (LCMV), the prototype arenavirus, and Lassa virus (LASV), causative agent of Lassa hemorrhagic fever (LHF), belong to the Old World group of the family Arenaviridae. Both viruses have extensive strain diversity and significant variations in lethality and pathogenicity for man and expe...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0385-9
更新日期:2004-12-01 00:00:00
abstract::Rabbit kidney epithelial cells (RK13) exhibited a cytopathic effect (CPE) characterized by cell rounding after infection with human cytomegalovirus (HCMV). Although HCMV-specific immediate early and early antigens were detected by indirect immunofluorescence techniques, neither late antigens nor infectious progeny vir...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314121
更新日期:1985-01-01 00:00:00