Abstract:
:Avian influenza due to highly pathogenic avian influenza (HPAIV) H5N1 virus is not a food-borne illness but a serious panzootic disease with the potential to be pandemic. In this study, broiler chickens were vaccinated with commercial H5N1 or H5N2 inactivated vaccines prior to being challenged with an HPAIV H5N1 (clade 2.2.1 classic) virus. Challenged and non-challenged vaccinated chickens were kept together, and unvaccinated chickens served as contact groups. Post-challenge samples from skin and edible internal organs were collected from dead and sacrificed (after a 14-day observation period) birds and tested using qRT-PCR for virus detection and quantification. H5N1 vaccine protected chickens against morbidity, mortality and transmission. Virus RNA was not detected in the meat or edible organs of chickens vaccinated with H5N1 vaccine. Conversely, H5N2 vaccine did not confer clinical protection, and a significant virus load was detected in the meat and internal organs. Phylogenetic analysis showed that the H5N1 virus vaccine and challenge virus strains are closely related. The results of the present study strongly suggest a need for proper selection of vaccines and their routine evaluation against newly emergent field viruses. These actions will help to reduce human exposure to HPAIV H5N1 virus from both infected live birds and slaughtered poultry. In addition, rigorous preventive measures should be put in place in order to minimize the public-health risks of avian influenza at the human-animal interface.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Hassan MK,Kilany WH,Abdelwhab EM,Arafa AS,Selim A,Samy A,Samir M,Le Brun Y,Jobre Y,Aly MMdoi
10.1007/s00705-012-1242-xsubject
Has Abstractpub_date
2012-05-01 00:00:00pages
951-9issue
5eissn
0304-8608issn
1432-8798journal_volume
157pub_type
杂志文章abstract::A reverse transcription-polymerase chain reaction (RT-PCR) procedure for the detection of avian, duck, and goose reovirus (ARV, DRV, and GRV) RNA from cell culture supernatant and clinical samples was established. Based on multiple sequence alignment, a pair of degenerate primers was selected and synthesized. The ampl...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-006-0731-1
更新日期:2006-08-01 00:00:00
abstract::The genus Bromovirus currently contains six species whose members have relatively narrow host ranges. In the present work, a new bromovirus infecting elderberry (Sambucus nigra L.) is reported. dsRNA was purified and sequenced by next-generation sequencing, and with minimal additional completion by Sanger sequencing, ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3629-1
更新日期:2018-02-01 00:00:00
abstract::Herpes simplex virus type 2 (HSV-2), strain YS-4 C-1, isolated by plaque cloning from a clinical isolate was found to be resistant to acyclovir (ACV; acycloguanosine) in vitro. It was sensitive to phosphonoacetic acid and 9-beta-D-arabinofuranosyladenine. Thymidine kinase (TK) activity of YS-4 C-1 was less than 1% of ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310998
更新日期:1988-01-01 00:00:00
abstract::The disease caused by Newcastle disease virus (NDV) is a severe threat to the poultry industry worldwide. Recently, NDV has been isolated in the Antarctic region. Detailed studies on the mode of evolution of NDV strains isolated worldwide are relevant for our understanding of the evolutionary history of NDV. For this ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2434-y
更新日期:2015-08-01 00:00:00
abstract::RNA extracted from 120 leaf specimens from 17 plant species was pooled, and polyadenylated RNA species were sequenced together without barcoding in one lane using massively parallel sequencing technology. After analysis, complete or partial genome sequences representing 20 virus isolates of 16 polyadenylated RNA speci...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-1166-x
更新日期:2012-02-01 00:00:00
abstract::Lolium latent virus (LoLV) was recently detected in the USA for the first time in ryegrass hybrids (Lolium perenne x Lolium multiflorum). The genome of one USA isolate, LoLV-US1, has now been fully sequenced. The positive strand genomic RNA is 7674 nucleotides (nt) long and is organized in five open reading frames (OR...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0108-8
更新日期:2008-01-01 00:00:00
abstract::Immunofluorescence assay using monospecific and monoclonal antibodies to the 65 K major protein of human cytomegalovirus (HCMV) was carried out to monitor the expression of this protein in infected cells. Regardless of differences in the reactivity of the monoclonal antibodies, as determined by immunoblotting and immu...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314653
更新日期:1988-01-01 00:00:00
abstract::Reassortant H1 swine influenza viruses (SIVs) carrying 2009 pandemic H1N1 virus (pH1N1) genes have been isolated from pigs worldwide. Seven novel reassortant H3N2 SIVs were identified from diseased pigs in the USA from winter 2010 to spring 2011. These novel viruses contain three or five internal genes from pH1N1 and ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-1203-9
更新日期:2012-03-01 00:00:00
abstract::Reassortants between a low-pathogenic avian influenza virus strain A/Duck/Primorie/2621/2001 (H5N2) and a high-yield human influenza virus strain A/Puerto Rico/8/34 (H1N1) were generated, genotyped and analyzed with respect to their yield in embryonated chicken eggs, pathogenicity for mice, and immunogenicity. A reass...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-006-0931-8
更新日期:2007-01-01 00:00:00
abstract::Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), a disease that has worldwide distribution. Whilst it has been eradicated in most of Western Europe and Scandinavia, it remains a problem in other regions, particularly Eastern Europe and South America. For this study, in 2013, 24 cat...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3241-4
更新日期:2017-06-01 00:00:00
abstract::Here, we infected mice with cytopathic bovine viral diarrhea virus 1 (cp BVDV1) by oral inoculation and investigated the effects of infection by histopathological, immunohistochemical (IHC), hematological methods. Twelve mice were infected, and samples were obtained at day 2, 5, and 9 postinfection (pi). Most of the i...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2649-y
更新日期:2016-02-01 00:00:00
abstract::The complete genome sequence of grapevine Bulgarian latent virus (GBLV) has been determined. RNA-1 (7,452 nt in length) contains a single ORF of 6,285 nt, encoding a polyprotein with conserved motifs characteristic of the viral protease cofactor (Prot-cofact), the NTP-binding protein (NTP), the cysteine-like protease ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0908-5
更新日期:2011-05-01 00:00:00
abstract::The sequence of the gene encoding the membrane protein of human coronavirus 229 E (HCV 229 E) has been determined. The primary translation product, deduced from the DNA sequence, is a polypeptide of 225 amino acids with a predicted molecular weight of 26,000. The polypeptide has 3 potential N-glycosylation sites. Many...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317928
更新日期:1989-01-01 00:00:00
abstract::A total of 472 samples from domestic pigs collected in China from 2015 to 2018 were tested for the presence of porcine circovirus types 2 and 3 (PCV2 and PCV3, respectively) by conventional polymerase chain reaction analysis. The prevalence of PCV2, PCV3, and PCV2/3 co-infection was 50.0%, 13.3%, and 6.78%, respective...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04336-4
更新日期:2019-10-01 00:00:00
abstract::The complement fixing antigens of rubella virus have been found to contain virion envelope but little nucleocapsid polypeptides. They are also shown to be associated with two additional polypeptides of host origin. Evidence is presented to suggest that the complement fixing antigens are structural components of the pl...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315301
更新日期:1986-01-01 00:00:00
abstract::We investigated the transforming domain of a recently isolated feline sarcoma virus (TP1-FeSV) which encodes a fgr-related tyrosine kinase expressed as a gag-fgr fusion protein. The gag portion was removed and replication-competent expression vectors (RCAS) with inserted v-fgr sequences were established. Chicken embry...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01379113
更新日期:1994-01-01 00:00:00
abstract::Since the appearance of resistance to antiretroviral treatment is unavoidable, the host cell's transcription factor NF-kappaB is a novel HIV target. The goal of this study was to characterize the effect of two immunomodulators, curcumin (Cur) and sulfasalazine (Sul), with a protease inhibitor, indinavir (IDV), on HIV-...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-007-0023-4
更新日期:2008-01-01 00:00:00
abstract::Both H9N2 subtype avian influenza and 2009 pandemic H1N1 viruses (pH1N1) can infect humans and pigs, which provides the opportunity for virus reassortment, leading to the genesis of new strains with potential pandemic risk. In this study, we generated six reassortant H9 viruses in the background of three pH1N1 strains...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2009-3
更新日期:2014-07-01 00:00:00
abstract::Yellow fever was presumably imported to the Americas from West-Africa from the 16th to the 19th century. American and African genotypes of the virus are distinguishable, indicating separate evolution in different vector/host cycles. The complete nucleotide sequence of the yellow fever virus strain TRINID79A, isolated ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050708
更新日期:1999-01-01 00:00:00
abstract::Here we describe the results of experimental infections of captive big brown bats (Eptesicus fuscus) with three newly isolated bat lyssaviruses from Eurasia (Aravan, Khujand, and Irkut viruses). Infection of E. fuscus was moderate (total, 55-75%). There was no evidence of transmission to in-contact cage mates. Incubat...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-005-0785-0
更新日期:2006-10-01 00:00:00
abstract::Nucleocapsid protein (NP) gene of Newcastle disease virus (NDV) strains mainly isolated in Japan from 1930 to 2001 was genetically characterized. By deduced amino acid sequence comparison, the N-terminal region (from 1 to 401 residues) of the NP protein was found to be highly conserved, while the C-terminal region was...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0309-8
更新日期:2004-08-01 00:00:00
abstract::A physical map for the genome of Spodoptera littoralis (Egyptian cottonworm) nuclear polyhedrosis virus of B-type (S1MNPV-B) [Cherry CL, Summers MD (1985) J Invertebr Pathol 46: 289-295] was prepared using the restriction endonucleases SmaI, SfiI, NotI, ApaI, KpnI, BstEII, PstI, SacI, and HindIII. The size of the geno...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01313815
更新日期:1989-01-01 00:00:00
abstract::Algeria is the largest country of Africa, with a population of 40 million inhabitants living in disparate environments from the Sahara to the large cities of the Mediterranean coast. The molecular epidemiology of hepatitis B virus (HBV) variants has been partially described, but variations in the seroprevalence of HBV...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3284-6
更新日期:2017-06-01 00:00:00
abstract::The small intestine of piglets orally infected with rotavirus was examined by electron microscopy 18, 24, 48 and 60 hours after infection. At 18 and 24 hours after infection columnar epithelial cells covered the villi. Infected epithelial cells tended to be less electron-dense than uninfected cells and were more numer...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317902
更新日期:1979-01-01 00:00:00
abstract::The complete nucleotide sequence of Pelargonium flower break virus (PFBV) has been determined. The genomic RNA is 3923 nucleotides (nt) long and contains five open reading frames (ORFs). The 5'-proximal ORF encodes a 27 kDa protein (p27) and terminates with an amber codon which may be read-through into an in-frame p56...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0231-5
更新日期:2004-03-01 00:00:00
abstract::Murine monoclonal antibodies against the fusion (F) and hemagglutinin-neuraminidase (HN) proteins of Sendai virus (SV) were prepared and studied on their antiviral activities, particularly on the neutralization of infectivity. On the analysis with solid phase competitive ELISA, 26 anti-HN antibodies were divided into ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01316735
更新日期:1986-01-01 00:00:00
abstract::Nanoviruses are multi-component plant-infecting single-stranded DNA viruses. Using a viral metagenomics-informed approach, a new nanovirus and two associated alphasatellite molecules have been identified in an uncultivated asymptomatic Vicia cracca plant in the Rhône region of France. This novel nanovirus genome inclu...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3634-4
更新日期:2018-03-01 00:00:00
abstract::Helper component protease (HC-Pro) is a potyvirus-encoded multifunctional protein and a major determinant of symptom expression in a susceptible plant. Here, we show the involvement of clover yellow vein virus (ClYVV) HC-Pro in necrotic symptom expression in broad bean (Vicia faba cv. Wase). In this host, lethal necro...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-007-1073-3
更新日期:2008-01-01 00:00:00
abstract::Chikungunya fever is self-limiting. However, neurological and hemorrhagic complications have been seen in recent outbreaks. The clinical manifestations of this disease are similar to those of dengue virus infection, indicating the need for differential diagnosis in areas such as India, which are endemic for both virus...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2829-4
更新日期:2016-06-01 00:00:00
abstract::An F35L mutation in the N-terminal domain of the polymerase acidic protein (PA-Nter), which contains the active site of the endonuclease, has been reported to result in higher polymerase activity in mouse-adapted strains of the 2009 pandemic influenza A H1N1 virus. We modeled wild and mutant complexes of uridine 5'-mo...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3681-x
更新日期:2018-04-01 00:00:00