Abstract：
:Nucleocapsid protein (NP) gene of Newcastle disease virus (NDV) strains mainly isolated in Japan from 1930 to 2001 was genetically characterized. By deduced amino acid sequence comparison, the N-terminal region (from 1 to 401 residues) of the NP protein was found to be highly conserved, while the C-terminal region was highly variable among the NDV isolates. A phylogenetic tree construct based on the nucleotide sequence of the complete NP gene revealed that the old (prior to 1970s) and the new (after 1980s) isolates could be classified into two major different groups, i.e., a group comprising virulent strains, and another group composed of avirulent strains. By restriction enzyme analysis using Pst I, none of the virulent strains were cleaved, while avirulent strains were cleaved. The results may be useful for simple primary screening test for differentiating NDV isolates.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Pham HM,Chang KS,Mase M,Ohashi K,Onuma Mdoi
10.1007/s00705-004-0309-8keywords:
subject
Has Abstractpub_date
2004-08-01 00:00:00pages
1559-69issue
8eissn
0304-8608issn
1432-8798journal_volume
149pub_type
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