Sequence variation in two genes determines the efficacy of transmission of citrus tristeza virus by the brown citrus aphid.

Abstract:

:Vector transmission is an important part of the viral infection cycle, yet for many viruses little is known about this process, or how viral sequence variation affects transmission efficacy. Here we examined the effect of substituting genes from the highly transmissible FS577 isolate of citrus tristeza virus (CTV) in to the poorly transmissible T36-based infectious clone. We found that introducing p65 or p61 sequences from FS577 significantly increased transmission efficacy. Interestingly, replacement of both genes produced a greater increase than either gene alone, suggesting that CTV transmission requires the concerted action of co-evolved p65 and p61 proteins.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Harper SJ,Killiny N,Tatineni S,Gowda S,Cowell SJ,Shilts T,Dawson WO

doi

10.1007/s00705-016-3070-x

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

3555-3559

issue

12

eissn

0304-8608

issn

1432-8798

pii

10.1007/s00705-016-3070-x

journal_volume

161

pub_type

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