Identification of two nucleotide sequence sub-groups within Potato mop-top virus.

Abstract:

:To evaluate the variation of Potato mop-top pomovirus from potato fields, 21 isolates were collected from different Danish locations. Reverse transcription-polymerase chain reaction-restriction fragment length polymorphism (RT-PCR-RFLP) of regions of RNA2 was performed for all 21 isolates resulting in the establishment of two sub-groups of isolates. The nucleotide sequence of a region encoding part of the 'readthrough protein' of RNA2 was compared for 9 of these isolates. This sequence analysis confirmed the RT-PCR-RFLP grouping. The isolates were tested for symptom expression in indicator plants and grouped according to symptom development. No correlation between grouping based on symptom development and genotype was observed.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Nielsen SL,Nicolaisen M

doi

10.1007/s00705-002-0926-z

keywords:

subject

Has Abstract

pub_date

2003-02-01 00:00:00

pages

381-8

issue

2

eissn

0304-8608

issn

1432-8798

journal_volume

148

pub_type

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