Abstract:
:We evaluated the importance of dendritic cells (DCs) in the induction of the immune response after immunization of mice with DNA plasmid Auxo-GTU(®)-MultiHIV. First, GTU(®)-encoded protein was shown to be expressed by DCs of the draining lymph nodes (LNs) following intradermal (i.d.) immunization. Next, donor mice were immunized with the MultiHIV DNA plasmid, and DCs were enriched and further used to immunize naïve recipient mice. For the first time, the results show that i.d. immunization with Auxo-GTU(®)-MultiHIV transfects DCs in vivo, enabling them to present antigens and induce HIV-specific immune responses in recipient mice.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Malm M,Krohn K,Blazevic Vdoi
10.1007/s00705-011-1003-2subject
Has Abstractpub_date
2011-09-01 00:00:00pages
1607-10issue
9eissn
0304-8608issn
1432-8798journal_volume
156pub_type
杂志文章abstract::The ability of lymphocytes from patients with multiple sclerosis (MS) to produce Interferon (IFN-alpha) in response to Newcastle Disease Virus (NDV) was studied in vitro. The correlation between individual IFN-alpha titers and natural killer (NK) cell activity and the presence of HLA system antigens associated with MS...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314877
更新日期:1982-01-01 00:00:00
abstract::Pepper chlorotic spot virus (PCSV), newly found in Taiwan, was identified as a new tospovirus based on the molecular characterization of its S RNA. In this study, the complete M and L RNA sequences of PCSV were determined. The M RNA has 4795 nucleotides (nts), encoding the NSm protein of 311 aa (34.5 kDa) in the viral...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3260-1
更新日期:2017-07-01 00:00:00
abstract::Cell-mediated immune response was studied in experimental herpetic infection in mice. Our data suggest that herpes simplex virus induced immunosuppression in mice. This depression was documented by the unresponsiveness of the lymphocytes to PHA stimulation in vitro and by the enhanced susceptibility of adult mice to C...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF02175597
更新日期:1975-01-01 00:00:00
abstract::Cell cultures inoculated with caprine respiratory syncytial virus (RSV) were studied with light, fluorescent, and electron microscopy to determine the morphogenesis and structure of the virus. Small syncytia were seen after 36 hours in culture. After 48 hours in culture, syncytia were large and numerous and pleomorphi...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314543
更新日期:1980-01-01 00:00:00
abstract::The leafhopper Euscelidius variegatus is a natural vector of chrysanthemum yellows phytoplasma (CY) and an efficient vector of flavescence dorée phytoplasma (FD) under laboratory conditions. During a transcriptome sequencing (RNA-seq) project aimed at investigating the interactions between the insect and the two phyto...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-3158-3
更新日期:2017-03-01 00:00:00
abstract::Four detection techniques, three of which gave reliable identification of the virus particles, were used to locate potato leafroll virus (PLRV) in the alimentary canal of its main aphid vector, Myzus persicae Sulz: immunofluorescence on cryostat sections, conventional transmission electron microscopy on ultrathin sect...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01378639
更新日期:1993-01-01 00:00:00
abstract::In attempts to further develop murine leukemia virus (MLV) based retroviral vectors for gene therapy, we investigated vector production and antisense expression from retroviral constructs with U3 deletions or insertions. Promoter elements in the U3 region of the 3' LTR of the vector pLXSN were deleted and replaced wit...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050170029
更新日期:2001-01-01 00:00:00
abstract::The genomes of two neurovirulent strains of poliovirus type 3, wild type P3/Leon/37 and a vaccine revertant P3/119/70, have been cloned in E. coli. The cDNA: RNA hybrid method used was efficient and may have wide applicability for cDNA cloning. Overlapping clones spanning the entire genome were obtained for each strai...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309297
更新日期:1984-01-01 00:00:00
abstract::A full-length cDNA clone of olive latent virus 1 (OLV-1), a member of the genus Necrovirus, family Tombusviridae, was subjected to site-directed mutagenesis, and coat protein gene mutants were constructed. A mutant clone, denoted Delta3297, was obtained by deleting the nucleotide in position 3297, thus inducing a fram...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-006-0767-2
更新日期:2006-10-01 00:00:00
abstract::Rabbit anti-idiotypic antibodies (anti-ids) were generated against three bank vole and one human monoclonal antibody (MAb) specific for the two envelope glycoproteins of Puumala virus (G1 and G2). The anti-ids were purified by sequential immunoaffinity chromatography. Each anti-id inhibited the antigen binding of its ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309537
更新日期:1993-01-01 00:00:00
abstract::Severe fever with thrombocytopenia syndrome (SFTS) is caused by SFTS virus (SFTSV). Although SFTS originated in China, it is an emerging infectious disease with prevalence confirmed in Japan, Korea, and Vietnam. The full-length genomes of 51 Korean SFTSV isolates from 2013 to 2016 were sequenced, and the sequences wer...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04733-0
更新日期:2020-11-01 00:00:00
abstract::We constructed a recombinant baculovirus encoding a dengue (DEN)-2 virus envelope glycoprotein truncated of 102 amino acids (aa) at its C-terminus (D2E delta 102). The production, processing and transportation of the recombinant protein in baculovirus-infected Spodoptera frugiperda (Sf9) cells and its immunogenic prop...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309465
更新日期:1994-01-01 00:00:00
abstract::The complete nucleotide sequence of the viral protein 2 (VP2) ORF was determined for 26 Vietnamese infectious bursal disease isolates collected from clinical outbreaks in vaccinated flocks from 1987 to 2018 and two commercial vaccine specimens. These sequences were compared for molecular classification with 42 referen...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04287-w
更新日期:2019-08-01 00:00:00
abstract::The genomic sequence of an El Amar isolate of plum pox virus (PPV) from Egypt was determined by sequencing overlapping cDNA fragments. This is the first complete sequence of a member of the El Amar (EA) strain of PPV. The genome consists of 9791 nt, excluding a poly(A) tail at the 3' terminus. The complete nt sequence...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-005-0703-x
更新日期:2006-06-01 00:00:00
abstract::Begomovirus infection is becoming a threat in fresh-market tomato in Argentina, where mixed infections with begomoviruses are common. The complete sequence of a begomovirus isolate infecting tomato sampled in Salta was molecularly characterized. Phylogenetic analysis showed that this virus isolate is closely related t...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1355-2
更新日期:2012-10-01 00:00:00
abstract::Northern pike from several locations in central Canada were observed to have two different types of hyperplastic epidermal lesions on the body and fins. One type of epidermal hyperplasia consisted of flat lesions, bluish-white in color with a granular or "gritty" appearance. Histological examination of this granular l...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310815
更新日期:1984-01-01 00:00:00
abstract::A rice protein homologous to eukaryotic translation elongation factor 1A (eEF-1A) was found to interact with the Pns6 of rice ragged stunt virus (RRSV), the type member of the genus Oryzavirus, family Reoviridae, in yeast two-hybrid screening. The interaction between the rice protein, designated OseEF-1A, and RRSV Pns...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1703-x
更新日期:2013-10-01 00:00:00
abstract::The complete genomes of tobamovirus isolates from Plantago major L. from New Zealand (NZ-439), Plantago sp. from Germany (Kons 1105), Actinidia chinensis (Actinidia-AC) and A. deliciosa (Actinidia-AD) from China were sequenced and compared to previously published tobamovirus genomes. Their genome organization and phyl...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1292-0
更新日期:2012-07-01 00:00:00
abstract::Whilst bovine leukemia virus (BLV) causes considerable economic losses to the dairy industry worldwide, information on its molecular epidemiology and economic impact in beef cattle is limited. Here, blood from 880 animals from Zambia's major cattle-rearing provinces was screened for BLV by nested PCR. Positive pools w...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04350-6
更新日期:2019-10-01 00:00:00
abstract::A total of 34 highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) strains isolated from Vietnam during 2013-2014 were sequenced and analyzed. A partial sequence of ORF1a corresponding to the nonstructural protein 2 (Nsp2) coding region and the full sequence of open reading frame 5 (ORF5) g...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2699-1
更新日期:2016-03-01 00:00:00
abstract::Usurpation of the host's signalling pathways is a common strategy employed by viruses to promote their successful replication. Here we show that infection with the orthopoxvirus vaccinia virus (VACV) leads to sustained stimulation of c-Jun activity during the entire infective cycle. This stimulation is temporally regu...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3446-6
更新日期:2017-10-01 00:00:00
abstract::We have studied the biological and molecular characteristics of a MNSV isolate collected in Spain (MNSV-Malpha5) and generated a full-length cDNA clone from which infectious RNA transcripts can be produced. The host range of MNSV-Malpha5 appeared to be limited to cucurbits and did not differ from that of MNSV-Dutch [4...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-002-0927-y
更新日期:2003-03-01 00:00:00
abstract::The base sequences of the coding region of the nucleoprotein (NP) genes of two H 10 influenza A viruses, one avian (virus N) and one mink virus, have been determined by primer extension. When the NP genes and the NP sequences derived from the only open reading frame of the two H 10 viruses were compared with those of ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01319816
更新日期:1988-01-01 00:00:00
abstract::Some of the properties of three continuous cell lines derived from BK virus-induced hamster tumors were examined. The cell lines had in vitro growth characteristics of transformed cells. Morphologically most of the cells were fibroblastic, but multinucleated giant cells were also common. Ultrastructurally all three ce...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01318002
更新日期:1976-01-01 00:00:00
abstract::The genomic RNA of infectious flacherie virus (IFV), an insect picornavirus, was shown to contain a covalently attached substance which can be labeled with 125I-Bolton and Hunter reagent. This substance was found to be a protein covalently linked to the virus genome, because it cosedimented and was copurified with the...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317378
更新日期:1986-01-01 00:00:00
abstract::Rice black-streaked dwarf virus (RBSDV) is an economically important virus that causes maize rough dwarf disease and rice black-streaked dwarf disease in East Asia. To study RBSDV variation and recombination, we examined the segment 9 (S9) sequences of 49 RBSDV isolates from maize and rice in China. Three S9 recombina...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2291-0
更新日期:2015-04-01 00:00:00
abstract::Reassortants between a low-pathogenic avian influenza virus strain A/Duck/Primorie/2621/2001 (H5N2) and a high-yield human influenza virus strain A/Puerto Rico/8/34 (H1N1) were generated, genotyped and analyzed with respect to their yield in embryonated chicken eggs, pathogenicity for mice, and immunogenicity. A reass...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-006-0931-8
更新日期:2007-01-01 00:00:00
abstract::Several species in the genus Datura (family Solanaceae) are hosts for begomoviruses (family Geminiviridae), both in the New World and the Old World. Here, we report the genome characterization of two isolates of a novel Old World monopartite begomovirus infecting Datura innoxia in Sudan, which we propose to be named "...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3574-z
更新日期:2018-01-01 00:00:00
abstract::The bisegmented genome of a putative double-stranded (ds) RNA virus from Ustilaginoidea virens was sequenced and analyzed. The larger genomic segment of 2112 bp encodes a putative RNA-dependent RNA polymerase (RdRp, 628 aa), and the smaller one of 2082 bp encodes a putative coat protein (CP) of 539 aa. The 5' untransl...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-1991-9
更新日期:2014-07-01 00:00:00
abstract::The characterization of the aetiological agent of viral erythrocytic infection (VEI) of sea bass suggests a retroviral origin of the disease. RNA from viral erythrocytic infection virus (VEIV) and DNA from blood and organs of VEI-affected fish hybridized to a specific retrovirus cDNA probe. Sequences homologous to ret...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309960
更新日期:1995-01-01 00:00:00