Abstract：
:The Sindbis replicon expression system was used to express La Crosse (LAC) virus envelope glycoprotein genes in both mammalian and mosquito cell culture. Replicon expressed LAC proteins had correct molecular mass (Mr) and were antigenically similar to wild type LAC envelope proteins. In addition, LAC G1 and G2 proteins colocalized when expressed from separate constructs in both mammalian and mosquito cells suggesting that they were trafficked through the cell similarly to wild type LAC proteins. A truncated form of the G1 protein was secreted from mosquito cells when expressed alone. The truncated G1 protein was also secreted from mosquito cells when expressed with the G2 protein, but to a lesser extent than when expressed alone, suggesting that the G2 protein sequestered G1 protein intracellularly. The Sindbis replicon system is a powerful tool for the study of LAC virus protein maturation within mosquito cells and mosquitoes.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Kamrud KI,Olson KE,Higgs S,Carlson JO,Beaty BJdoi
10.1007/s007050050381subject
Has Abstractpub_date
1998-01-01 00:00:00pages
1365-77issue
7eissn
0304-8608issn
1432-8798journal_volume
143pub_type
杂志文章abstract：:Eleven cloned North American pseudorabies virus (PRV) strains and the European vaccine strains K and BUK were characterized by their thermal sensitivity and rabbit virulence markers. Heat sensitivity of the strains and isolates ranged from the highly heat resistant strain K, to the extremely heat labile strain BUK and...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01318093
更新日期：1979-01-01 00:00:00
abstract：:Nanoviruses are multi-component plant-infecting single-stranded DNA viruses. Using a viral metagenomics-informed approach, a new nanovirus and two associated alphasatellite molecules have been identified in an uncultivated asymptomatic Vicia cracca plant in the Rhône region of France. This novel nanovirus genome inclu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3634-4
更新日期：2018-03-01 00:00:00
abstract：:The complete genome of ϕ16, a temperate corynephage from Corynebacterium glutamicum ATCC 21792, was sequenced and annotated (GenBank: KY250482). The electron microscopy study of ϕ16 virion confirmed that it belongs to the family Siphoviridae. The ϕ16 genome consists of a linear double-stranded DNA molecule of 58,200 b...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3383-4
更新日期：2017-08-01 00:00:00
abstract：:Strains of an influenza H10N4 virus have been isolated during an outbreak of a respiratory disease in mink on the south-east coast of Sweden. This was the first example of a disease in mammals caused by the H10 subtype. We compared the A/mink/Sweden/84 strain with two recent avian H10N4 isolates, one from fowl and ano...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01318353
更新日期：1990-01-01 00:00:00
abstract：:Whilst bovine leukemia virus (BLV) causes considerable economic losses to the dairy industry worldwide, information on its molecular epidemiology and economic impact in beef cattle is limited. Here, blood from 880 animals from Zambia's major cattle-rearing provinces was screened for BLV by nested PCR. Positive pools w...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04350-6
更新日期：2019-10-01 00:00:00
abstract：:Despite reports that the PB1-F2 protein contributes to influenza virus pathogenicity in the mouse model, little is known about its significance in avian hosts. In our previous study, the A/Vietnam/1203/04 (H5N1) wild-type virus (wtVN1203) was more lethal to mallard ducks than a reverse genetics (rg)-derived VN1203. In...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0666-4
更新日期：2010-06-01 00:00:00
abstract：:The complete genome sequence of a Chinese very virulent infectious bursal disease virus (vvIBDV) strain, Harbin-1, was determined. Based on the sequence analysis, the molecular characteristics and potential virulence determinants and origin of vvIBDV strains were identified. Phylogenetic analysis indicated that a reas...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0140-8
更新日期：2008-01-01 00:00:00
abstract：:Bubaline alphaherpesvirus 1 (BuHV1) is a member of the family Herpesviridae, subfamily Alphaherpesvirinae, genus Varicellovirus. To date, no full genome sequence of BuHV has been published. Here, we report the complete genome sequence of bubaline alphaherpesvirus 1 (BuHV1) strain b6 (BuHV1-b6), isolated from a water b...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3218-8
更新日期：2017-05-01 00:00:00
abstract：:The biological function of entomopoxvirus (EPV) spindles (inclusion bodies that lack virions), has not been elucidated. We characterized the function of EPV spindles in the cupreous chafer, Anomala cuprea (Coleoptera: Scarabaeidae). Spheroids or spheroids mixed with spindles of Anomala cuprea EPV were administered per...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070103
更新日期：2000-01-01 00:00:00
abstract：:The yeast two-hybrid system has been used to identify domains of the Newcastle disease virus (NDV) phosphoprotein (P) involved in self-association and interaction with the nucleocapsid protein (NP). Deletion analysis was used to map the domain(s) of the P protein involved in P:P and P:NP interactions. The C-terminal 4...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0439-z
更新日期：2005-03-01 00:00:00
abstract：:This study examines viral protein and DNA synthesis in Spodoptera frugiperda multicapsid nuclear polyhedrosis virus (SfMNPV) infections of S. frugiperda and Trichoplusia ni cells. A total of 28 infected cell specific polypeptides (ICSPs) were detected in the productive S. frugiperda cells. Of these, 14 were identified...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310626
更新日期：1990-01-01 00:00:00
abstract：:Multidrug-resistant uropathogenic Escherichia coli (UPEC) is increasing gradually on a worldwide scale. We therefore examined the possibility of bacteriophage (phage) therapy for urinary tract infections (UTIs) caused by the UPEC strains as an alternative to chemotherapy. In addition to the well-known T4 phage, KEP10,...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0031-4
更新日期：2008-01-01 00:00:00
abstract：:Chilo iridescent virus (CIV; the type strain of the genus Iridovirus) replicates productively in larvae of the boll weevil, Anthonomus grandis. This study focuses on characterizing productive infections of a boll weevil cell line, BRL-AG-3A (AG3A), starting with CIV reared in the waxworm, Galleria mellonella. We show ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1417-5
更新日期：2012-11-01 00:00:00
abstract：:Hepatitis B virus (HBV) is one of the most prevalent and infectious agents that leads to liver disease in humans. Five clinical forms of HBV infection exist, including fulminant, acute, chronic, asymptomatic and occult. The chronic, asymptomatic and occult forms are long-term infections that can lead to hepatocellular...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-014-2002-x
更新日期：2014-08-01 00:00:00
abstract：:The present study on genetic diversity of human papillomaviruses in women infected by HIV in Brazil describes the frequency, the genotypes, and five new variants of HPV. One hundred fifty cervical smears of HIV-positive women were subjected to cytological examination, and the DNA samples obtained were assayed by MY09/...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0828-6
更新日期：2007-01-01 00:00:00
abstract：:Complete nucleotide (nt) and deduced amino acid sequences of two onion yellow dwarf virus (OYDV) isolates showing mild and severe symptoms in onion but being unable to infect garlic were determined. The genomes consisted of 10,459 and 10,461 nt (without the 3' poly(A) tail) and were 92.2 % identical. Comparison of the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1597-z
更新日期：2013-06-01 00:00:00
abstract：:Brefeldin A (BFA) induced a rapid redistribution of vesicular stomatitis virus G protein (VSV-G) to the endoplasmatic reticulum (ER) in interferon (IFN)-pretreated cells. This result is consistent with accumulation of VSV-G in the trans-Golgi (GC) complex in cells pretreated with IFN and implies that IFN does not inte...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314635
更新日期：1992-01-01 00:00:00
abstract：:This work describes the first molecular characterization of grapevine virus B (GVB) in Portuguese grapevine cultivars. During a routine screening of 44 accessions in the National Collection of Grapevine Varieties (CAN PRT051), 17 were found infected with GVB in DAS-ELISA assays with commercial antibodies. However, onl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3024-3
更新日期：2016-12-01 00:00:00
abstract：:Mouse hepatitis virus 3 (MHV-3) is highly hepatotropic in sensitive mice. Temperature-sensitive mutants (ts mutants) induced by N-methyl-N'-nitrosoguanidine and 5-fluorouracil were isolated. Twelve mutants which were able to induce the formation of syncytia at 33 degrees C but not at the restrictive temperature (39.5 ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01487679
更新日期：1988-01-01 00:00:00
abstract：:Infectivity of non-occluded particles of nuclear polyhedrosis virus infecting Galleria mellonella L. was determined with bioassay technique in vivo and with plaque assay method in vitro using SCLd 135 (Quiot) established cell culture. A plaque-forming virus unit corresponds to about 50 ID50 for Galleria larvae injecte...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315023
更新日期：1979-01-01 00:00:00
abstract：:Synthesis of murine gamma interferon (MuIFN gamma) by phytohemagglutinin (PHA)-stimulated spleen cells was inhibited in a dose-dependent manner by graded concentrations of tunicamycin or 2-deoxy-glucose, both of which inhibit glycosylation. The homologous (murine) and heterologous (rat) antiviral activities of the MuI...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310659
更新日期：1984-01-01 00:00:00
abstract：:During infection host histo-blood group antigens (HBGAs) act as attachment factors that interact with rabbit hemorrhagic disease virus (RHDV) and participate in the infectious process. In the present study, baculovirus expressing recombinant RHDV capsid protein (VP60r) as a vaccine immunogen was used to test its antig...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3509-8
更新日期：2017-11-01 00:00:00
abstract：:To evaluate the variation of Potato mop-top pomovirus from potato fields, 21 isolates were collected from different Danish locations. Reverse transcription-polymerase chain reaction-restriction fragment length polymorphism (RT-PCR-RFLP) of regions of RNA2 was performed for all 21 isolates resulting in the establishmen...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0926-z
更新日期：2003-02-01 00:00:00
abstract：:Sendai virus given intravenously into CFLP mice produced dose-related suppression of the acute inflammation produced by carrageenan and 5-HT. The inhibition correlated with interferon levels in the blood, suggesting that interferon may account for the virus-induced anti-inflammatory effect. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314606
更新日期：1981-01-01 00:00:00
abstract：:Amino acids Lys34, His36, and Phe37 were substituted by PCR-mediated, site-directed mutagenesis for three Trp's in the AcNPV polyhedrin sequence. Phase contrast microscopy revealed refringent, amorphous polyhedra in the nuclei of infected cells. Electron microscopy confirmed a great variation in form and size of the m...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050675
更新日期：2000-01-01 00:00:00
abstract：:Three sets of primers to detect foot-and-mouth disease virus (FMDV) using multiplex RT-PCR were designed based on several reference nucleotide sequences, and their reaction conditions were determined. By testing ten-fold serial dilutions of FMDV, the sensitivity of multiplex RT-PCR is 100 times higher than conventiona...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-1082-y
更新日期：2008-01-01 00:00:00
abstract：:The viral erythrocytic infection (VEI) of sea bass (Dicentrarchus labrax) is evidenced by the presence of inclusion bodies in the erythrocytes and erythroblasts. Virus-like particles (VEIV) about 135-150 nm in diameter were observed in erythroblasts of affected fish. No culturable virus could be recovered from the org...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310951
更新日期：1991-01-01 00:00:00
abstract：:Genomic information about Muscovy duck parvovirus is still limited. In this study, the genome of the pathogenic MDPV strain YY was sequenced. The full-length genome of YY is 5075 nucleotides (nt) long, 57 nt shorter than that of strain FM. Sequence alignment indicates that the 5' and 3' inverted terminal repeats (ITR)...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2946-0
更新日期：2016-09-01 00:00:00
abstract：:Konjak mosaic virus (KoMV) belongs to the genus Potyvirus, family Potyviridae. The complete nucleotide sequence of KoMV F isolate (KoMV F) was determined. The genome is 9,544 nucleotides long excluding the 3' terminal poly A tail and encodes a typical potyviral 350-kDa polyprotein of 3,087 amino acids. Phylogenetic an...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0735-x
更新日期：2006-08-01 00:00:00
abstract：:MicroRNAs (miRNAs) are a new class of well-conserved small noncoding RNAs that mediate posttranscriptional gene regulation. Hepatitis B virus (HBV) causes various liver diseases, including chronic hepatitis, liver cirrhosis and hepatocellular cancer. Recent data have indicated HBV alters miRNAs expression patterns, bu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2286-x
更新日期：2015-03-01 00:00:00
