Abstract：
:Amino acids Lys34, His36, and Phe37 were substituted by PCR-mediated, site-directed mutagenesis for three Trp's in the AcNPV polyhedrin sequence. Phase contrast microscopy revealed refringent, amorphous polyhedra in the nuclei of infected cells. Electron microscopy confirmed a great variation in form and size of the mutated polyhedra. Although crystallization of the mutated polyhedrin occurred, it was irregular within each polyhedron. Virion occlusion was also severely affected. Virions were partially occluded, or only one virion was occluded per polyhedron. Results suggest that the substitution of these three amino acids affected the morphology of polyhedra, the uniformity of crystallization within each polyhedron, and the virion occlusion.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Bravo-Patiño A,Ibarra JEdoi
10.1007/s007050050675keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
827-34issue
4eissn
0304-8608issn
1432-8798journal_volume
145pub_type
杂志文章abstract：:The complete nucleotide sequence of Pelargonium flower break virus (PFBV) has been determined. The genomic RNA is 3923 nucleotides (nt) long and contains five open reading frames (ORFs). The 5'-proximal ORF encodes a 27 kDa protein (p27) and terminates with an amber codon which may be read-through into an in-frame p56...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0231-5
更新日期：2004-03-01 00:00:00
abstract：:Triple gene block 1 (TGB1) and coat protein (CP) sequences of 30 hosta virus X (HVX) isolates from Tennessee (TN), USA, were determined and compared with available sequences in GenBank. The CPs of all known HVX isolates, including those from TN, shared 98.3-100% and 98.2-100% nucleotide and amino acid sequence identit...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0522-6
更新日期：2009-01-01 00:00:00
abstract：:Mentha x gracilis 'Variegata', an ornamental mint clone first described about 200 years ago, exhibits virus-like vein banding symptoms. Double-stranded RNA and virion isolations revealed the presence of three viruses in a 'Variegata' plant. Cloning and sequencing disclosed that one of the viruses was a previously unid...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0586-x
更新日期：2006-01-01 00:00:00
abstract：:A novel iflavirus, tentatively named "Sogatella furcifera honeydew virus 1" (SFHV1), discovered through transcriptome sequencing analysis of white-backed planthoppers (Sogatella furcifera) collected in southern China, is described here. The full genome of SFHV1 is 10,837 nucleotides (nt) long, including the polyA tail...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3817-7
更新日期：2018-07-01 00:00:00
abstract：:A method for purification of Hog Cholera Virus (HCV) is presented. Cell-associated virus was extracted from PK15 cells 17 hours p.i. by fluorocarbon treatment. The virus was concentrated by polyethylene glycol precipitation and partially purified by pelleting on a fluorocarbon cushion. Final purification was achieved ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314377
更新日期：1977-01-01 00:00:00
abstract：:The cytomorphologic method previously reported for titration of adenoviruses has been employed for estimating the infectious titer of simian virus 40 (SV 40). Infected cells forming intranuclear inclusions were determined. The method examined possesses a number of advantages over virus titration by plaque assay and cy...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01341731
更新日期：1982-01-01 00:00:00
abstract：:The thymidine kinase (TK) of herpesviruses, in contrast to cellular TKs, phosphorylates a variety of substrates including antiherpetic nucleoside analogues. This study reports the identification and DNA sequence of the simian varicella virus (SVV) TK gene. A 32P-labeled varicella zoster virus (VZV) TK DNA probe hybrid...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718587
更新日期：1996-01-01 00:00:00
abstract：:Codiaeum variegatum (common name, garden croton) is an ornamental plant grown for its bright yellow variegated leaf morphology. Two C. variegatum plants with upward leaf curling and vein swelling symptoms were collected in Faisalabad, Pakistan. Sequencing of clones obtained by PCR amplification with specific primers s...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04633-3
更新日期：2020-08-01 00:00:00
abstract：:Localization of neutralizing, serotype specific epitopes of infectious bronchitis virus has been difficult because these epitopes are conformationally dependent. We identified amino acids involved in a serotype specific, conformationally dependent epitope by analysis of the S1 gene of 13 monoclonal antibody-neutraliza...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050239
更新日期：1997-01-01 00:00:00
abstract：:The envelope protein of human immunodeficiency virus type 1 (HIV-1) comprises the outer gp 120 SU domain and the anchoring gp41 TM domain, and the conventional view is that it has a single transmembrane region with the following C-terminal sequence situated entirely within the virion. However, we have recently propose...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170199
更新日期：2001-01-01 00:00:00
abstract：:Poultry production is an important sector of agriculture in Sri Lanka; however, there is a lack of information regarding circulation of infectious bronchitis virus (IBV). RNA was extracted from chicken tissues, subjected to IBV S1 RT-PCR, and sequenced. Overall, 19 out of 34 (55.88 %) samples were IBV positive and con...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2831-x
更新日期：2016-06-01 00:00:00
abstract：:Brugmansia suaveolens mottle virus (BsMoV) was the first potyvirus isolated from "angel trumpet" (Brugmansia suaveolens), described in Brazil. In this study, the complete nucleotide (nt) genome sequence of BsMoV was determined, and the deduced amino acid (aa) sequence was analyzed. The BsMoV RNA genome consists of 987...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0798-6
更新日期：2010-10-01 00:00:00
abstract：:In cultured chicken embryo fibroblasts treated with copper-(copper cation 10-minus 3.5-10-minus 5 M), nickel and cobalt salts an enhancement of the plating efficiency of Semliki Forest virus could be observed. This augmented plaque formation was most probably due to a higher adsorption rate of virions to the cell surf...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315593
更新日期：1975-01-01 00:00:00
abstract：:Among the four flavonoids tested quercetin and morin proved to be significantly effective against lethal Mengo virus-induced encephalitis in mice when the drugs were adminsitered orally (p.o.). With subcutaneous (s.c.) administration all four drugs failed to prevent mortality in the infected mice. Quercetin produced m...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315089
更新日期：1978-01-01 00:00:00
abstract：:The in vitro cytopathology and the neurovirulence to inbred mice demonstrated by five variants originally derived from one laboratory strain (Miyama) of type 1 herpes simplex virus (HSV-1) were studied comparatively. Three of the variants are syncytial [+GC (LPV), +GC (SPV), +GC (81)] and two are non-syncytial [-GCr a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317369
更新日期：1986-01-01 00:00:00
abstract：:In order to improve the knowledge of the humoral immune response to CMV infection, we developed an immunoblotting technique which allowed a better analysis of the changes in the pattern of anti CMV-polypeptides IgM. We examined 234 sera belonging to 27 renal allograft recipients developing a primary or recurrent CMV i...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310938
更新日期：1989-01-01 00:00:00
abstract：:The EDS adenovirus produced either in the allantoic cavity of embryonated duck eggs (A0 virus) or in chicken embryo liver cell cultures (TC0 virus) were comparatively studied by gradient ultracentrifugation in CsCl. The A0 viral particles banded at densities of 1.36 and 1.31 g/ml, whereas, the TC0 viral particle were ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314574
更新日期：1981-01-01 00:00:00
abstract：:Bovine lactoferrin (LF) and ribavirin (Rbv) were tested as antiviral agents against Seoul type hantavirus (SR-11 strain) in vitro. Hantaviral foci number in Vero E6 cells infected with SR-11 was reduced with LF treatment by 5 days post infection to obtain a 50% effective dose (ED50) of 2500 microg/ml, while pretreatme...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070077
更新日期：2000-01-01 00:00:00
abstract：:Reverse transcription coupled with the polymerase chain reaction (RT-PCR) was used for the rapid laboratory diagnosis of pestivirus infections. A direct DNA sequencing method was developed for the analysis of the amplified cDNA from the 5' noncoding region of the viral genome. 70 pestivirus strains were compared in th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309467
更新日期：1994-01-01 00:00:00
abstract：:Recently we have described that the Herpes simplex virus (HSV)-induced thymidine kinase (TK) induces AMP- and ADP-dThd-5'-phosphotransferase activities. We now demonstrate the heterogeneity of the described activities in isoelectric focusing experiments and polyacrylamide gel electrophoresis. A TK--mutant of HSV type ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314875
更新日期：1982-01-01 00:00:00
abstract：:Had-2, a mouse mutant cell line derived from FM3A, constitutively releases interferon-alpha and beta and acquires resistance to Newcastle disease virus (NDV) and other viruses. However, Had-2 was found as susceptible to Sendai virus (HVJ) as FM3A. Even when Had-2 cells were infected simultaneously with NDV and HVJ, on...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718199
更新日期：1996-01-01 00:00:00
abstract：:We detected a novel feline stool-associated circular DNA virus (FeSCV) in fecal samples from cats with diarrhea using consensus primers matching those of circovirus and cyclovirus. FeSCV is a circular DNA virus containing a genome with a total length of 2,046 nt encoding 2 open reading frames. Phylogenetic analyses in...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-4020-6
更新日期：2018-12-01 00:00:00
abstract：:Serological detection of isolated anti-hepatitis B core antibody (anti-HBc) can occur in various scenarios, but the most clinically relevant situation is occult hepatitis B virus (HBV) infection (OBI). The purpose of this study was to evaluate the prevalence and clinical relevance of isolated anti-HBc and of OBI with ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1810-8
更新日期：2014-02-01 00:00:00
abstract：:Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. In 2013, a large outbreak was reported on the archipelago of French Polynesia. In this study, we report the detection and molecular characterization of Zika virus for the first time in Chile from an outbreak among the inhabitant...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2695-5
更新日期：2016-03-01 00:00:00
abstract：:Synthetic peptides comprising the predicted principal neutralizing determinant (PND) in new African and North American HIV-1 clones were tested in ELISA for reactivity with ninety six serum samples from asymptomatic donors in six selected localities in Uganda. Irrespective of the geographical origin of the samples, th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322666
更新日期：1995-01-01 00:00:00
abstract：:To elucidate changes associated with the attenuated virulence in a modified live porcine reproductive and respiratory syndrome (PRRS) vaccine (Boehringer Ingelheim Animal Health, St. Joseph, MO), derived from an American prototype ATCC virus VR-2332, nucleotide sequence of 3' genome covering open reading frames (ORFs)...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050316
更新日期：1998-01-01 00:00:00
abstract：:Since the appearance of resistance to antiretroviral treatment is unavoidable, the host cell's transcription factor NF-kappaB is a novel HIV target. The goal of this study was to characterize the effect of two immunomodulators, curcumin (Cur) and sulfasalazine (Sul), with a protease inhibitor, indinavir (IDV), on HIV-...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0023-4
更新日期：2008-01-01 00:00:00
abstract：:Cross hemagglutination inhibition (HI) and neuraminidase inhibition (NI) tests demonstrated strong antigenic relationship between A/Swine/Wisconsin/1/73 (SW/73) and A/Swine/Shope/15/31 (SW/31) influenza viruses. An eigthyone fold purification of virus was achieved by adsorption and elution followed by differential ult...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317967
更新日期：1975-01-01 00:00:00
abstract：:Hepatitis B virus (HBV) is one of the most prevalent and infectious agents that leads to liver disease in humans. Five clinical forms of HBV infection exist, including fulminant, acute, chronic, asymptomatic and occult. The chronic, asymptomatic and occult forms are long-term infections that can lead to hepatocellular...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-014-2002-x
更新日期：2014-08-01 00:00:00
abstract：:During the epidemic outbreak in the region of Greifswald in the winter 1974/75, we found influenza virus variants which showed differences in the electrophoretic mobility of HA. Among the 25 isolates 13 were of slower and 12 of higher mobility. HA1 of 6 isolates was studied by determining the number of the carbohydrat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310484
更新日期：1991-01-01 00:00:00
