Abstract:
:Bovine lactoferrin (LF) and ribavirin (Rbv) were tested as antiviral agents against Seoul type hantavirus (SR-11 strain) in vitro. Hantaviral foci number in Vero E6 cells infected with SR-11 was reduced with LF treatment by 5 days post infection to obtain a 50% effective dose (ED50) of 2500 microg/ml, while pretreatment with LF was highly efficacious having an ED50 of 39 microg/ml. Conversely, 1 h pretreatment with Rbv revealed no inhibition of viral focus formation but could significantly reduce the number of viral foci (ED50: 10 microg/ml) when used from the time of viral infection. One hour pre-treatment of the cell monolayer with LF and subsequent addition of Rbv revealed a synergistic anti-hantaviral effect against SR-11, <20 FFU/ml as compared to 10(5) foci/ml in the control. One hour treatment of SR-11 with LF prior to cell inoculation gave an ED50 of 312.5 microg/ml. Whereas, washing the LF-pretreated cell monolayer with PBS demonstrated minimal focus reduction, suggesting LF lightly adheres to cells. These results indicate that LF has anti-hantaviral activity in vitro and inhibition of virus adsorption to cells which play an important role in revealing the anti-hantaviral activity of LF. This paper reports for the first time the anti-hantaviral effect of LF.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Murphy ME,Kariwa H,Mizutani T,Yoshimatsu K,Arikawa J,Takashima Idoi
10.1007/s007050070077keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
1571-82issue
8eissn
0304-8608issn
1432-8798journal_volume
145pub_type
杂志文章abstract::To investigate the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) in Northern Ireland, the ORF5 gene from nine field isolates was sequenced and phylogenetically analysed. The results revealed relatively high diversity amongst isolates, with 87.6-92.2% identity between farms at the nuc...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3886-7
更新日期:2018-10-01 00:00:00
abstract::RNA extracted from 120 leaf specimens from 17 plant species was pooled, and polyadenylated RNA species were sequenced together without barcoding in one lane using massively parallel sequencing technology. After analysis, complete or partial genome sequences representing 20 virus isolates of 16 polyadenylated RNA speci...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-1166-x
更新日期:2012-02-01 00:00:00
abstract::The ability of human fibroblast interferon to suppress colony formation and papovavirus T-antigen expression in six human tumor cell lines did not appear to correlate with their content of chromosomes 16 and 21. Additional factors or chromosomes may be involved in the manifestation of interferon action in aneuploid ce...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317505
更新日期:1979-01-01 00:00:00
abstract::We present here the results of the analysis of the complete genome sequence of a lytic bacteriophage, vB_ButM_GuL6, which is the first virus isolated from Buttiauxella. Electron microscopy revealed that vB_ButM_GuL6 belongs to the family Myoviridae, order Caudovirales. The genome of vB_ButM_GuL6 is a linear, circularl...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04780-7
更新日期:2020-11-01 00:00:00
abstract::Human metapneumovirus (hMPV) causes both upper and lower respiratory tract infection worldwide. Because little is known about the estimated level of exposure to hMPV in Taiwan, data for 1999 are presented. One thousand one hundred twenty sera from otherwise healthy community-based subjects aged 1 month to 99 years wer...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0596-1
更新日期:2010-03-01 00:00:00
abstract::The VP1 encoded by genomic segment B of birnaviruses is generally known to exist as a genome-linked protein (VPg) and as a "free" polypeptide of 90 kDa in virus particles. The guanylylation activity associated with infectious bursal disease virus (IBDV) was demonstrated by incubating purified virus in presence of [alp...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050154
更新日期:1997-01-01 00:00:00
abstract::We have determined the complete nucleotide sequence of three isolates (two severe and one attenuated) of the potyvirus Leek yellow stripe virus (LYSV), the main causal agent of the garlic mosaic disease that is prevalent in Aomori prefecture, northern Japan. They contained 10,296-10,297 nucleotides and encoded a deduc...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0482-9
更新日期:2005-06-01 00:00:00
abstract::The complete genome sequence of a Chinese very virulent infectious bursal disease virus (vvIBDV) strain, Harbin-1, was determined. Based on the sequence analysis, the molecular characteristics and potential virulence determinants and origin of vvIBDV strains were identified. Phylogenetic analysis indicated that a reas...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0140-8
更新日期:2008-01-01 00:00:00
abstract::Evidence is presented that the excess pool of penton complexes purified from adenovirus type 1-infected HEp-2 cells also carry an endonucleolytic activity. The effect was measured with both E. coli and adenovirus type 1 double-stranded DNA substrates. The use of agarose gels for assaying the endonuclease activity allo...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315620
更新日期:1976-01-01 00:00:00
abstract::Dengue and chikungunya viruses co-circulate and cause infections that start with similar symptoms but progress to radically different outcomes. Therefore, an early diagnostic test that can differentiate between the two is needed. A single-step multiplex real-time RT-PCR assay was developed that can simultaneously dete...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2217-x
更新日期:2015-01-01 00:00:00
abstract::Although cell-mediated cytotoxicity effectively kills target virus-infected cells, no careful consideration has been given to the fate of infectious progeny virus contained within target cells following the cytolytic event. To address this issue with respect to murine cytomegalovirus (MCMV) pathogenesis, we developed ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0459-8
更新日期:2005-04-01 00:00:00
abstract::We examined the pattern of the N gene-mediated systemic hypersensitive response (HR), which was induced by tobacco mosaic virus upon temperature shift, and analyzed the distribution of the coat protein and the virus-encoded 126 kDa replicase protein (126 K protein) by immunoblot analysis. In the middle- and lower-posi...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0373-0
更新日期:2004-11-01 00:00:00
abstract::Synthesis of the Epstein-Barr virus (EBV) associated early antigen (EA) can be induced by a variety of agents in Raji cells, a latently EBV-infected Burkitt lymphoma line. We investigated the role of lipoproteins in this EA induction system. Cell growth was not affected by lipoprotein-deficiency, but EA induction by m...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01313877
更新日期:1989-01-01 00:00:00
abstract::A wheat-infecting furovirus found in Tompkins County, New York, U.S.A. was identified as a strain of Soil-borne wheat mosaic virus (SBWMV) by means of sequence analyses of portions of its RNA 1 and 2. The nucleotide sequences of several of its genes differed by c. 9 to 12% from those of the corresponding genome region...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050200011
更新日期:2002-03-01 00:00:00
abstract::We have recently demonstrated the geographic isolation of rice tungro bacilliform virus (RTBV) populations in the tungro-endemic provinces of Isabela and North Cotabato, Philippines. In this study, we examined the genetic structure of the virus populations at the tungro-outbreak sites of Lanao del Norte, a province ad...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050070013
更新日期:2000-01-01 00:00:00
abstract::Rabies is one of the most important zoonotic diseases and is caused by several rabies virus (RABV) variants. These variants can exhibit differences in neurovirulence, and few studies have attempted to evaluate the neuroinvasiveness of variants derived from vampire bats and wild carnivores. The aim of this study was to...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3530-y
更新日期:2017-12-01 00:00:00
abstract::Genomic characterization of Norwalk-like human caliciviruses (NLVs) originating from outbreaks and sporadic cases of acute gastroenteritis has revealed surprisingly high levels of diversity, even in the RNA polymerase gene, which is anticipated to be highly conserved. Since information on antigenic relationship is lim...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050020
更新日期:2000-01-01 00:00:00
abstract::We detected a novel feline stool-associated circular DNA virus (FeSCV) in fecal samples from cats with diarrhea using consensus primers matching those of circovirus and cyclovirus. FeSCV is a circular DNA virus containing a genome with a total length of 2,046 nt encoding 2 open reading frames. Phylogenetic analyses in...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-4020-6
更新日期:2018-12-01 00:00:00
abstract::A putative chrysovirus recovered from Brassica campestris var. purpurea and provisionally named "Brassica campestris chrysovirus 1" (BrcCV1) was sequenced. The genome of the putative BrcCV1 consists of three double-stranded RNAs (dsRNAs) comprising 3,639 (dsRNA 1), 3,567 (dsRNA 2) and 3,337 (dsRNA 3) base pairs, respe...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-3196-x
更新日期:2017-04-01 00:00:00
abstract::The complete nucleotide sequence of the viral protein 2 (VP2) ORF was determined for 26 Vietnamese infectious bursal disease isolates collected from clinical outbreaks in vaccinated flocks from 1987 to 2018 and two commercial vaccine specimens. These sequences were compared for molecular classification with 42 referen...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04287-w
更新日期:2019-08-01 00:00:00
abstract::NDV variants released from the early passaged BHK21/WI-2 cells persistently infected with NDV carry the structural antigen of a latent virus of BHK21/WI-2 cells as a result of phenotypic mixing between NDV and the latent virus. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317290
更新日期:1978-01-01 00:00:00
abstract::Chilo iridescent virus (CIV) belongs to the family Iridoviridae, which are icosahedral cytoplasmic DNA viruses with large, linear, and circularly permuted genomes. Previous studies on infected-cell-specific polypeptides suggested temporal regulation of CIV gene expression. Recently, we demonstrated three temporal clas...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0232-4
更新日期:2004-04-01 00:00:00
abstract::Electropherotypes (ET), serotypes, and subgroups of equine rotaviruses isolated from foals in Japan were determined. The ETs of 136 isolates from 1981 through to 1991 were divided into six groups: ET-A-ET-F. The ET-A, -B, -C, -D, -E, and -F were present in 3, 1, 121, 9, 1, and 1 strains, respectively. Representative v...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01379088
更新日期:1993-01-01 00:00:00
abstract::The genetic and antigenic diversity of group A rotaviruses recovered from Israeli infants has been expanded recently by the inclusion of three unusual human rotavirus strains. Two rotavirus strains (Ro-5829 and Ro-5960) were shown to be the first viruses outside Japan that resembled the AU-1 genogroup of feline like h...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01379138
更新日期:1994-01-01 00:00:00
abstract::Degenerate primers were used to detect and amplify 3'-terminal genome fragments of potyviruses from medicinal aroid plants growing at 16 sites in China. Virus was detected in 7 samples of which six, all of Pinellia ternata, contained a strain of soybean mosaic virus (SMV) similar to that previously reported from this ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0390-z
更新日期:2005-01-01 00:00:00
abstract::A new virus resembling members in the genus Carlavirus was identified in an Out of Yesteryear rose (Rosa sp.) by high-throughput sequencing. The virus was discovered during the screening of a rose virus collection belonging to Foundation Plant Services (UC-Davis). The full genome of the virus is 8825 nt long, excludin...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04864-4
更新日期:2021-01-01 00:00:00
abstract::Different patterns of disease were observed among 11 chimpanzees who were inoculated intravenously with hepatitis E virus (HEV) positive fecal specimens from four different outbreaks (Nepal 1981, Uzbekistan 1981, Pakistan 1985, and Mexico 1986). Five chimpanzees had marginal or no liver enzyme elevations within 70 day...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050070065
更新日期:2000-01-01 00:00:00
abstract::Dengue virus (DENV) causes a life-threatening illness, with a wide range of symptoms from mild febrile illness, dengue fever (DF), to life-threatening illness, dengue hemorrhagic fever (DHF). Antibody-dependent enhancement (ADE) is considered to be a risk factor for DHF. In the present study, we determined the paramet...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1787-3
更新日期:2014-01-01 00:00:00
abstract::Few reports are available on HCV molecular epidemiology among IDUs in Eastern Europe, and none in Montenegro. The aim of this study was to investigate the HCV genotype distribution in Montenegro among IDUs and to perform Bayesian and evolutionary analysis of the most prevalent HCV genotype circulating in this populati...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3224-5
更新日期:2017-06-01 00:00:00
abstract::Genetic relationships among echovirus type 25 (ECV 25) isolates associated with aseptic meningitis in Germany 1997/98 and a 40-year-old ECV 25 prototype strain were investigated using RT-PCR and direct sequencing the VP1/2A gene junction region. Sequences were compared to each other and to non-polio enterovirus repres...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050640
更新日期:1999-01-01 00:00:00