Abstract：
:Dengue and chikungunya viruses co-circulate and cause infections that start with similar symptoms but progress to radically different outcomes. Therefore, an early diagnostic test that can differentiate between the two is needed. A single-step multiplex real-time RT-PCR assay was developed that can simultaneously detect and quantitate RNA of all dengue virus (DENV) serotypes and chikungunya virus (CHIKV). The sensitivity was 100 % for DENV and 95.8 % for CHIKV, whilst the specificity was 100 % for both viruses when compared with conventional RT-PCR. The detection limit ranged from 1 to 50 plaque-forming units. The assay was successfully used for differential diagnosis of dengue and chikungunya in Pune, where the viruses co-circulate.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Cecilia D,Kakade M,Alagarasu K,Patil J,Salunke A,Parashar D,Shah PSdoi
10.1007/s00705-014-2217-xsubject
Has Abstractpub_date
2015-01-01 00:00:00pages
323-7issue
1eissn
0304-8608issn
1432-8798journal_volume
160pub_type
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