Abstract:
:Analysis of the preS1 gene of hepatitis B virus was used to define two nosocomial outbreaks of HBV infection. In an outbreak in an oncology unit we had previously shown by single stranded conformational polymorphism (SSCP) analysis of a 189 bp fragment of the preS1 gene, that 52 children were infected with HBV strains that displayed only 5 different SSCP profiles. Sequencing of a 383 bp fragment encompassing the entire preS1 gene, revealed that isolates with same SSCP profile were identical in sequence across the entire preS1 gene, confirming that those patients with the same SSCP pattern had epidemiologically linked infections. A second outbreak involved 8 liver transplant patients from two different hospitals, 5 of whom were from the same hospital at which the oncology outbreak had occurred. Two of these 5 patients had HBV strains that were identical to strains from the oncology unit and nosocomial transmission probably accounted for the infections in these two, while diversity of both SSCP profiles and sequence data of remaining 6 patients supported the conclusion that they had not been infected from a common source. The donor liver is believed to be the most likely source of infection in these patients. HBV isolates from patients infected in the community were used as a standard for the general degree of preS1 sequence variation of local HBV strains. Phylogenetic analysis and comparison with reference HBV clones revealed that of 27 local HBV strains, genotypes A and D occurred most frequently and were identified in 14 and 12 patients respectively, while genotype C was detected in one patient.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Hardie DR,Williamson Cdoi
10.1007/s007050050200subject
Has Abstractpub_date
1997-01-01 00:00:00pages
1829-41issue
9eissn
0304-8608issn
1432-8798journal_volume
142pub_type
杂志文章abstract::The DNA of mammalian cells treated with 4,5',8-trimethylpsoralen (Trioxsalen) is crosslinked (preventing transcription) only after photoactivation with near ultraviolet light. Suppression of gene action in cocultivated or fused cultures, including both induction and antiviral action of fibroblast interferon, can conse...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01320762
更新日期:1980-01-01 00:00:00
abstract::An assay to characterize plasma human immunodeficiency virus 1 (HIV-1) sequences for patients with low viral loads was developed by combining the selective binding of anti-CD44 MicroBeads with a nested RT-PCR targeting the env C2V4 region. Sequences were obtained from 10 of 20 HIV+ patients who had viral loads below 4...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0659-3
更新日期:2010-06-01 00:00:00
abstract::In the Mt. Kosciusko alpine area of Australia there are three well-separated populations of Cardamine lilacina, an endemic sward-forming perennial brassica, and these are infected with turnip yellow mosaic tymovirus. The genetic variation in these viral populations has been assessed by an RNA hybrid mismatch polymorph...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309845
更新日期:1993-01-01 00:00:00
abstract::We investigated the frequency and the clinical relevance of hepatitis B virus (HBV) pre-S mutations in Qidong, China. The results showed HBV pre-S mutants were detected in 48.4% (47/97) of patients with HBV infection. Both pre-S deletion and pre-S2 start codon mutations were more frequently found in HCC than in CH pat...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0176-9
更新日期:2008-01-01 00:00:00
abstract::The nonstructural rotavirus protein NSP1 binds specifically to viral mRNAs and to interferon regulatory factor 3 (IRF3), inducing IRF3 degradation through a proteasome-dependent pathway. By using a vaccinia virus expression system in mammalian cells, we found that the yield of NSP1 was 8- and 13-fold lower than the vi...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-006-0850-8
更新日期:2007-02-01 00:00:00
abstract::Because of an inherent dependence on host cell second and third messenger signaling pathways for activation of HIV-1 expression, a potential exists for a relationship between the induction of latent HIV-1 and cell-cycle-related events. To investigate this potential relationship, cellular models of latent HIV-1 infecti...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050144
更新日期:1997-01-01 00:00:00
abstract::The porcine paramyxovirus LPM recognizes alpha, but not beta, anomers of sialic acid containing structures, specifically sialyl (alpha 2,3) lactose. The virus specificity is directed to the sialyl residue and to the C'4 axial OH and the C'6 CH2OH of the galactose present in this structure. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309755
更新日期:1993-01-01 00:00:00
abstract::Function of rotavirus NSP1 was analyzed by using single-NSP1 gene-substitution reassortants, SKF, SDF, and SNF which have the NSP1 gene derived from human rotaviruses KU, DS-1, and canine rotavirus K9, respectively, in the genetic background of simian rotavirus SA11. The NSP1 genes from KU, DS-1, K9, and SA11 exhibite...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050600
更新日期:1999-01-01 00:00:00
abstract::Interleukin-2 dependent feline T-lymphoblastoid cells designated as MYA-1 cells were established. The cells were free from exogenous retroviruses and sensitive for replication of feline immunodeficiency virus (FIV). FIV can grow more efficiently in MYA-1 cells than in feline primary peripheral blood mononuclear cells....
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01313750
更新日期:1989-01-01 00:00:00
abstract::A new human endogenous retroviral family (HERV-F) has recently been identified from human chromosome 7q31.1-q31.3 that was identical to the XA34 cDNA clone isolated from a human glioma cDNA library with an ERV-9 env probe. We investigated pol fragments of the HERV-F family from the Old World monkeys (crab-eating monke...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s705-002-8327-7
更新日期:2002-01-01 00:00:00
abstract::The Chinese hog cholera lapinized virus (HCLV), also called the "Chinese strain" or "C-strain" of classical swine fever virus (CSFV), was developed in China in the 1950s. There are uncertainties about the genetic heterogeneity and origin of this vaccine virus. The objectives of this study were to investigate the genet...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-0948-5
更新日期:2011-06-01 00:00:00
abstract::Human adenovirus Ad36 is causally and correlatively associated in animals and humans, respectively, with increased adiposity and altered metabolic profile. We inoculated rats with Ad36 or UV-inactivated Ad36, or mock-infected them. Four days later, Ad36-infected rats showed 23% greater epididymal fat pad weight and vi...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0219-2
更新日期:2008-01-01 00:00:00
abstract::To characterize the role of the humoral immune response on HIV-1 infection of monocytes and macrophages (M phi s) we examined the susceptibility of in vitro cultured monocyte/M phi s to various HIV-1 isolates and the influence of heterologous and particularly autologous anti HIV-1 sera on this infection. Depending on ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01538815
更新日期:1994-01-01 00:00:00
abstract::The complete genome sequence of grapevine Bulgarian latent virus (GBLV) has been determined. RNA-1 (7,452 nt in length) contains a single ORF of 6,285 nt, encoding a polyprotein with conserved motifs characteristic of the viral protease cofactor (Prot-cofact), the NTP-binding protein (NTP), the cysteine-like protease ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0908-5
更新日期:2011-05-01 00:00:00
abstract::Changes in the NP gene of H5N1 highly pathogenic avian influenza (HPAI) viruses have previously been shown to affect viral replication, alter host gene expression levels and affect mean death times in infected chickens. Five amino acids at positions 22, 184, 400, 406, and 423 were different between the two recombinant...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0399-4
更新日期:2009-01-01 00:00:00
abstract::vp1, a gene encoding one of the capsid proteins of Taura syndrome virus, was cloned into the pGEX-6P-1 expression vector, and the resulting construct was then used to transform E. coli strain BL21. After induction, an N-terminally glutathione-S-transferase-tagged VP1 (GST-VP1) protein with a molecular mass of 80 kDa w...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1460-2
更新日期:2013-01-01 00:00:00
abstract::The chemical nature of the binding sites for vesicular stomatitis virus (VSV) was studied by measuring the ability of solubilized rat brain cell membranes (SRBM) to compete with cultured cells for viral binding. SRBM significantly reduced both binding and infectivity of VSV. After separation of protein and lipid compo...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01311075
更新日期:1988-01-01 00:00:00
abstract::A factor secreted from avian cells infected non productively with a non cytopathogenic mutant of vesicular stomatitis virus (VSV ts 1026) interferes with HIV replication in CEM cells and peripheral blood monocytes (PBL). Production of infectious particles is decreased and many virions lack cores and/or spikes. In CEM ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317266
更新日期:1992-01-01 00:00:00
abstract::The leafhopper Euscelidius variegatus is a natural vector of chrysanthemum yellows phytoplasma (CY) and an efficient vector of flavescence dorée phytoplasma (FD) under laboratory conditions. During a transcriptome sequencing (RNA-seq) project aimed at investigating the interactions between the insect and the two phyto...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-3158-3
更新日期:2017-03-01 00:00:00
abstract::Usurpation of the host's signalling pathways is a common strategy employed by viruses to promote their successful replication. Here we show that infection with the orthopoxvirus vaccinia virus (VACV) leads to sustained stimulation of c-Jun activity during the entire infective cycle. This stimulation is temporally regu...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3446-6
更新日期:2017-10-01 00:00:00
abstract::Each of 20 gilts (principals) from a commercial swine herd free of antibody for porcine parvovirus (PPV) was exposed intranasally and orally to PPV at the onset of gestation. The gilts were killed and necropsied 22 +/- 1 days later to determine the effect of the virus on their embryos. An equal number of gilts (contro...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01340540
更新日期:1980-01-01 00:00:00
abstract::Porcine circovirus type 2 (PCV2) is a member of the genus Circovirus in the family Circoviridae. Most subgenomic molecules of PCV2 have been mapped. Here, the first full-length sequence of a subviral molecule of PCV2 (CH-IVT12) containing a reverse complement sequence of the PCV2 genome was determined by sequencing DN...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2859-y
更新日期:2016-07-01 00:00:00
abstract::Complete nucleotide sequences of three strains (I, III, and IV) of Barley yellow mosaic virus (BaYMV) isolated in Japan were determined. The length of the genome was the same among the three strains; RNA1 was 7,642 nt and RNA2 was 3,585 nt. The molecular phylogenetic analysis showed that strain I was most closely rela...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0163-1
更新日期:2008-01-01 00:00:00
abstract::To elucidate changes associated with the attenuated virulence in a modified live porcine reproductive and respiratory syndrome (PRRS) vaccine (Boehringer Ingelheim Animal Health, St. Joseph, MO), derived from an American prototype ATCC virus VR-2332, nucleotide sequence of 3' genome covering open reading frames (ORFs)...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050316
更新日期:1998-01-01 00:00:00
abstract::Equine herpesvirus 1 (EHV-1) is one of the most significant pathogens that affects equine species worldwide, causing sporadic abortion, neonatal deaths, chorioretinopathy, as well as neurological and upper respiratory tract diseases. Currently, conventional PCR targeting different genes is used widely for the molecula...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3379-0
更新日期:2017-08-01 00:00:00
abstract::A novel strain of Japanese yam mosaic virus (JYMV-CN) was identified in a yam plant with foliar mottle symptoms in China. The complete genomic sequence of JYMV-CN was determined. Its genomic sequence of 9701 nucleotides encodes a polyprotein of 3247 amino acids. Its organization is virtually identical to that of two J...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2231-z
更新日期:2015-02-01 00:00:00
abstract::Several species in the genus Datura (family Solanaceae) are hosts for begomoviruses (family Geminiviridae), both in the New World and the Old World. Here, we report the genome characterization of two isolates of a novel Old World monopartite begomovirus infecting Datura innoxia in Sudan, which we propose to be named "...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3574-z
更新日期:2018-01-01 00:00:00
abstract::Five avian paramyxoviruses of serotype 8 (APMV-8) were isolated during a study monitoring wild birds in Kazakhstan in 2013 and each was further characterized. The viruses were isolated from three White-fronted geese (Anser albifrons), one Whooper swan (Cygnus cygnus), and one Little stint (Calidris minuta). Before our...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3593-9
更新日期:2018-02-01 00:00:00
abstract::The oncogene E6 of human papillomavirus 8, which is associated with skin cancers in epidermodysplasia verruciformis, was transcribed and translated in vitro. The resulting 17 kDa protein did not bind to the cellular p53 in contrast to E6 of HPV16. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309654
更新日期:1992-01-01 00:00:00
abstract::Porcine circovirus type 2 (PCV2) is the essential infectious agent causing porcine circovirus-associated disease (PCVD) in pigs and one of the important viruses that severely jeopardize the swine husbandry industry. PCV2 elicits the unfolded protein response (UPR) via activation of the PERK pathway, and its capsid pro...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04185-1
更新日期:2019-05-01 00:00:00