Inhibition of phorbol ester-induced neutrophil chemiluminescence by FeLV.

Abstract:

:Receptor-mediated activation is accompanied by phospholipid metabolism and by calcium fluctuation resulting in a chemiluminescence (CL) response in the neutrophil. This pathway involves activation of protein kinase C (PKC) and the NADPH oxidase. Artificial stimulants such as phorbol esters, specifically 12-O-tetradecanylphorbol-13-acetate (TPA), circumvent the receptor-mediated pathway and activate PKC resulting in a measurable CL response. Neutrophils from feline leukemia virus (FeLV) exposed cats were tested for their ability to generate a TPA-induced CL response. As compared to the non-FeLV-exposed specific-pathogen-free (SPF) control cat neutrophil CL responses, both viremic and nonviremic FeLV-exposed cats showed significant decreases in their CL responsiveness. Neither ultraviolet light-inactivated FeLV (UV-FeLV) nor protein components (FeLV-p15E and FeLV-p27) caused a significant decrease in the CL responses of the SPF cat neutrophils. The suppressed TPA-induced CL response from FeLV-infected cats may involve an intracellular mechanism not affected in vitro by exposure of the neutrophil to the virus or viral components.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Dezzutti CS,Lafrado LJ,Lewis MG,Olsen RG

doi

10.1007/BF01310506

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

75-85

issue

1-2

eissn

0304-8608

issn

1432-8798

journal_volume

111

pub_type

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