Abstract:
:Receptor-mediated activation is accompanied by phospholipid metabolism and by calcium fluctuation resulting in a chemiluminescence (CL) response in the neutrophil. This pathway involves activation of protein kinase C (PKC) and the NADPH oxidase. Artificial stimulants such as phorbol esters, specifically 12-O-tetradecanylphorbol-13-acetate (TPA), circumvent the receptor-mediated pathway and activate PKC resulting in a measurable CL response. Neutrophils from feline leukemia virus (FeLV) exposed cats were tested for their ability to generate a TPA-induced CL response. As compared to the non-FeLV-exposed specific-pathogen-free (SPF) control cat neutrophil CL responses, both viremic and nonviremic FeLV-exposed cats showed significant decreases in their CL responsiveness. Neither ultraviolet light-inactivated FeLV (UV-FeLV) nor protein components (FeLV-p15E and FeLV-p27) caused a significant decrease in the CL responses of the SPF cat neutrophils. The suppressed TPA-induced CL response from FeLV-infected cats may involve an intracellular mechanism not affected in vitro by exposure of the neutrophil to the virus or viral components.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Dezzutti CS,Lafrado LJ,Lewis MG,Olsen RGdoi
10.1007/BF01310506subject
Has Abstractpub_date
1990-01-01 00:00:00pages
75-85issue
1-2eissn
0304-8608issn
1432-8798journal_volume
111pub_type
杂志文章abstract::Using gag protein of feline immunodeficiency virus (FIV) expressed in Escherichia coli, an enzyme-linked immunosorbent assay (ELISA) system was developed for detection of antibodies to FIV gag protein in cat sera. With serum samples from cats experimentally infected with several strains and an infectious molecular clo...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309815
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abstract::"Phages" include viruses of eubacteria and archaea. At least 5568 phages have been examined in the electron microscope since the introduction of negative staining in 1959. Most virions (96%) are tailed. Only 208 phages (3.7%) are polyhedral, filamentous, or pleomorphic. Phages belong to one order, 17 families, and thr...
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pub_type: 杂志文章
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更新日期:1997-01-01 00:00:00
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doi:10.1007/BF01314489
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更新日期:2001-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317902
更新日期:1979-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317531
更新日期:1975-01-01 00:00:00
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更新日期:2010-07-01 00:00:00
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更新日期:2019-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/BF01313885
更新日期:1989-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00705-007-1005-2
更新日期:2007-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/BF01315291
更新日期:1984-01-01 00:00:00
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更新日期:1996-01-01 00:00:00
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更新日期:1994-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-04-01 00:00:00
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更新日期:1988-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Archives of virology
pub_type: 杂志文章
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更新日期:1978-01-01 00:00:00
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更新日期:1997-01-01 00:00:00