Rapid visual detection of lily mottle virus using a loop-mediated isothermal amplification method.

Abstract:

:Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae) infects plants of the genus Lilium, causing a reduction in flower and bulb quality. A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed to detect the coat protein gene of LMoV. This LAMP method was highly specific for LMoV, with no cross-reaction with other lily viruses. The sensitivity of LMoV using the LAMP assay was 100 times more sensitive than that using conventional polymerase chain reaction. A reverse transcription LAMP (RT-LAMP) was then successfully applied to detect LMoV RNA. The newly established LAMP and one-step RT-LAMP provide an alternative method for detecting LMoV in lily plants.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Zhao B,Yang D,Zhang Y,Xu Y,Zhao X,Liang J,Fan X,Du Y,Zhu Z,Shi B,Zhang Q,Zhang X,Cai Y,Zhao K

doi

10.1007/s00705-017-3618-4

subject

Has Abstract

pub_date

2018-02-01 00:00:00

pages

545-548

issue

2

eissn

0304-8608

issn

1432-8798

pii

10.1007/s00705-017-3618-4

journal_volume

163

pub_type

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