Abstract:
:Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae) infects plants of the genus Lilium, causing a reduction in flower and bulb quality. A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed to detect the coat protein gene of LMoV. This LAMP method was highly specific for LMoV, with no cross-reaction with other lily viruses. The sensitivity of LMoV using the LAMP assay was 100 times more sensitive than that using conventional polymerase chain reaction. A reverse transcription LAMP (RT-LAMP) was then successfully applied to detect LMoV RNA. The newly established LAMP and one-step RT-LAMP provide an alternative method for detecting LMoV in lily plants.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Zhao B,Yang D,Zhang Y,Xu Y,Zhao X,Liang J,Fan X,Du Y,Zhu Z,Shi B,Zhang Q,Zhang X,Cai Y,Zhao Kdoi
10.1007/s00705-017-3618-4subject
Has Abstractpub_date
2018-02-01 00:00:00pages
545-548issue
2eissn
0304-8608issn
1432-8798pii
10.1007/s00705-017-3618-4journal_volume
163pub_type
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