Mapping of the functional domains of the alpha 4 protein of herpes simplex virus 1.

Abstract:

:Truncated alpha 4 genes were introduced into BHK tk- cells along with the neomycin phosphotransferase gene, that confers resistance to the eukaryotic antibiotic G418, driven by the HSV-1 beta tk promoter (beta tk- neor). Stably transformed cell lines were obtained and studied for the ability of the resident truncated alpha 4 genes to regulate the expression of the beta tk- neor, and for the ability of the truncated alpha 4 polypeptides to localize to the nuclei of transformed cells. The results indicated that the domain(s) for beta gene induction and for nuclear localization of the alpha 4 protein are located within the N-terminal 288 amino acids of the protein.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Sivropoulou A,Arsenakis M

doi

10.1007/BF01316907

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

317-25

issue

1-4

eissn

0304-8608

issn

1432-8798

journal_volume

129

pub_type

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