Abstract：
:A simple radial method based on the formerly described paper disc technique was developed for demonstration of neutralizing antibodies to enteroviruses with plaque forming ability. Sera administered by 5 microliter wire-loops to wells cut into the agar overlay of virus infected cell monolayers produced radial neutralization zones with a size directly related to their specific antibody contents. Results of poliovirus neutralizing antibody tests performed by the radial plaque neutralization method were found to be in a good agreement with those obtained in antibody titrations either in monolayer tube cultures or in microtitre system. The relatively simple technique makes possible to investigate several hundreds of serum samples in a single experiment, so it is especially suitable for use in expanded serological surveys. It proved to be useful also for identification of enteroviruses and for testing the laboratory strains for identity.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Nagy G,Takátsy Sdoi
10.1007/BF01317320subject
Has Abstractpub_date
1980-01-01 00:00:00pages
89-98issue
2eissn
0304-8608issn
1432-8798journal_volume
65pub_type
杂志文章abstract：:Meningitis is a serious condition that affects the central nervous system. It is an inflammation of the meninges, which is the membrane that surrounds both the brain and the spinal cord. Meningitis can be caused by bacterial, viral, or fungal infections. Many viruses, such as enteroviruses, herpesviruses, and influenz...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-020-04891-1
更新日期：2021-01-03 00:00:00
abstract：:Six synthetic peptides corresponding to regions of bovine enterovirus (BEV), strain VG-5-27, elicited antibodies in mice which reacted with the virus in various assays. These antibodies have been characterised on the basis of their ability to (1) neutralize the virus, (2) bind to the intact virus particle in an immuno...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309681
更新日期：1992-01-01 00:00:00
abstract：:MicroRNAs (miRNAs) are a new class of well-conserved small noncoding RNAs that mediate posttranscriptional gene regulation. Hepatitis B virus (HBV) causes various liver diseases, including chronic hepatitis, liver cirrhosis and hepatocellular cancer. Recent data have indicated HBV alters miRNAs expression patterns, bu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2286-x
更新日期：2015-03-01 00:00:00
abstract：:The '6K1' protein of the Pinellia isolate of Soybean mosaic virus was cloned into a prokaryotic expression vector and a polyclonal antiserum raised to the expressed fusion protein. In immunogold labeling of thin sections of infected leaves of Pinellia ternata, specific labeling occurred at the cell periphery. This mig...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0972-7
更新日期：2007-01-01 00:00:00
abstract：:The base sequences of the coding region of the nucleoprotein (NP) genes of two H 10 influenza A viruses, one avian (virus N) and one mink virus, have been determined by primer extension. When the NP genes and the NP sequences derived from the only open reading frame of the two H 10 viruses were compared with those of ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01319816
更新日期：1988-01-01 00:00:00
abstract：:Six isolates of Cassava brown streak virus (CBSV, genus Ipomovirus; Potyviridae) from the Lake Victoria basin in Uganda and Tanzania were characterized. Virus particles were 650 nm long. The complete coat protein (CP)-encoding sequences (1,101 nucleotides, nt) were 90.7-99.5 and 93.7-99.5% identical at the nt and amin...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0301-9
更新日期：2009-01-01 00:00:00
abstract：:Bovine herpesvirus 1 (BHV-1) is a major pathogen of cattle, causing significant disease including immunosuppression in infected animals. In vitro, the surface expression of major histocompatibility complex (MHC) class I molecules, crucial for an appropriate anti-viral immune response of the host, is down-regulated by ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070038
更新日期：2000-01-01 00:00:00
abstract：:Equine herpesvirus 1 glycoprotein D (EHV-1 gD) expressed constitutively in mammalian cell lines had similar electrophoretic mobility to gD produced in EHV-1 infected cells but lacked a possibly complexed higher molecular weight form seen in the latter. Recombinant gD was N-terminally cleaved at the same site as gD in ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718301
更新日期：1996-01-01 00:00:00
abstract：:Out of 136 new phages, 80 (59%) are classified into 23 species according to morphology and physicochemical properties. Six new species are described and species beta 4, from a previous classification scheme, is renamed T1. The morphology of 36 phage species is schematically represented. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:Brefeldin A (BFA) induced a rapid redistribution of vesicular stomatitis virus G protein (VSV-G) to the endoplasmatic reticulum (ER) in interferon (IFN)-pretreated cells. This result is consistent with accumulation of VSV-G in the trans-Golgi (GC) complex in cells pretreated with IFN and implies that IFN does not inte...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314635
更新日期：1992-01-01 00:00:00
abstract：:A reversed passive hemagglutination (RPHA) method was developed for the detection of bovine coronavirus in fecal specimens. Sheep erythrocytes fixed with glutaraldehyde, and then treated with tannic acid were coated with anti-bovine coronavirus rabbit antibodies purified by affinity chromatography using bovine coronav...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315291
更新日期：1984-01-01 00:00:00
abstract：:Since serological donor-screening tests for HIV were introduced in 1985, the safety of donated blood components has improved dramatically. However, these tests do not completely prevent the risk of transfusion-associated HIV infection related to the use of blood donated during the pre-seroconversion window period. Tes...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1085-x
更新日期：2011-11-01 00:00:00
abstract：:Rabbit anti-idiotypic antibodies (anti-ids) were generated against three bank vole and one human monoclonal antibody (MAb) specific for the two envelope glycoproteins of Puumala virus (G1 and G2). The anti-ids were purified by sequential immunoaffinity chromatography. Each anti-id inhibited the antigen binding of its ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309537
更新日期：1993-01-01 00:00:00
abstract：:Heterogeneity of 9 feline herpesvirus type 1 (FHV-1) strains consisting of the prototype C27 strain, one French isolate, six Japanese isolates, and the attenuated vaccine F2 strain was examined by biological, immunological, and molecular biological methods. No significant difference was observed in virus growth and an...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309701
更新日期：1992-01-01 00:00:00
abstract：:The acid-extractable leaf proteins of potato spindle tuber viroid (PSTV) infected tomato plants were analysed electrophoretically on polyacrylamide gels. The most prominent alteration found during disease development was the appearance of a "pathogenesis-related" protein with an apparent molecular weight of 14,000 (ca...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309998
更新日期：1984-01-01 00:00:00
abstract：:Mouse embryo fibroblasts and mouse adrenal tumor cells support the replication of encephalomyocarditis (EMC) virus, whereas rat glial and rat hepatoma cells are non-permissive. These differences in susceptibility were not due to the lack of virus attachment to rat cells. The findings that rat cells could be transfecte...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310884
更新日期：1986-01-01 00:00:00
abstract：:As a prerequisite for development of improved vaccines and diagnostic tools for control of the fish pathogen koi herpesvirus, or cyprinid herpesvirus 3 (CyHV-3), we have started to identify putative viral envelope and capsid proteins. The complete or partial CyHV-3 open reading frames ORF25, ORF65, ORF92, ORF99, ORF13...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2190-4
更新日期：2014-12-01 00:00:00
abstract：:Morphological, biological, serological, and molecular tests underpin the description of costus stripe mosaic virus (CoSMV) as a new member of the genus Potyvirus, family Potyviridae. Found affecting the native ornamental Costus spiralis in Brazil, the pathogen showed a severely restricted natural and experimental host...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04788-z
更新日期：2020-11-01 00:00:00
abstract：:Astroviruses are associated with acute gastroenteritis of human and many animal species. Recently, two studies have reported that novel astroviruses were found in bats. In order to extensively understand the genetic and phylogenetic characterization of bat astroviruses, we tested fecal samples of nine bat species that...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1011-2
更新日期：2011-08-01 00:00:00
abstract：:One host polypeptide (40,000 daltons) synthesized prior to infection is associated with the 250S RNA polymerase structure partially purified by a combination of velocity sedimentation and isopycnic separation. A series of pulse-chase experiments have shown that a 56,000 dalton polypeptide made during the eclipse phase...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317808
更新日期：1975-01-01 00:00:00
abstract：:In our attempt to isolate mutant cell lines resistant to Newcastle disease virus (NDV) we developed an improved procedure for enrichment of NDV-resistant cells from mouse FM3A cells and isolated a novel NDV-resistant mutant cell line, Had-2, with characteristics different from Had-1, a previously reported NDV-receptor...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310796
更新日期：1994-01-01 00:00:00
abstract：:Canine astrovirus (CAstV) is the causative agent of gastroenteritis in dogs. We collected rectal swabs from dogs with or without diarrhea symptoms in Japan and examined the feces for the presence of CAstV by RT-PCR with primers based on a conserved region of the ORF1b gene. The ORF1b gene of CAstV was not detected in ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2405-3
更新日期：2015-06-01 00:00:00
abstract：:An outbreak of a canine influenza virus (CIV) H3N2 reassortant derived from pandemic (pdm) H1N1 and CIV H3N2 in companion animals has underscored the urgent need to monitor CIV infections for potential zoonotic transmission of influenza viruses to humans. In this study, we assessed the virulence of a novel CIV H3N2 re...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2868-x
更新日期：2016-07-01 00:00:00
abstract：:In order to differentiate recent isolates of avian infectious bronchitis virus (IBV) in Taiwan, polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and direct sequencing methods were used to type 25 IBV Taiwan isolates. Two conserved sequences that flank the hypervariable region I (HVR I)...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718291
更新日期：1996-01-01 00:00:00
abstract：:Fowl adenoviruses (FAdVs) are widely distributed in chickens in Poland and throughout the world. FAdV infections have been reported in the United States, Australia, Europe, and the Mediterranean basin. Detection of FAdVs strains is very important from the epidemiological point of view and for monitoring disease outbre...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2635-4
更新日期：2016-01-01 00:00:00
abstract：:Non-structural as well as VP1 recombinant proteins of foot-and-mouth disease virus (FMDV) produced in E. coli, have been used to study the specific antibody response of infected or vaccinated swine. An analysis of sera from infected pigs, using a direct ELISA, showed that polypeptide 3ABC (spanning non-structural prot...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01538822
更新日期：1994-01-01 00:00:00
abstract：:Intravenous polyoma virus inoculation into pregnant Wistar rats resulted in transplacental infection of the foetus, causing tumours and hydronephroses. Cyclosporin A reduced these effects significantly. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313783
更新日期：1993-01-01 00:00:00
abstract：:The complete genome sequence of a novel duck orthoreovirus, designated DRV strain TH11(DRV-TH11), was determined and characterized. The DRV-TH11 genome is comprised of 23,417 bp and its genome organization is more similar to that of avian orthoreoviruses (ARVs) of chicken origin than other reoviruses. The results of c...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2241-x
更新日期：2015-01-01 00:00:00
abstract：:The complete genome of a novel mycovirus, Puccinia striiformis mitovirus 1 (PsMV1), derived from the wheat stripe rust fungus Puccinia striiformis strain SCSN-10, was sequenced and analyzed. The full-length cDNA sequence is 2496 bp in length with a predicted AU content of 57.65% in the genomic RNA. Sequence analysis i...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-04134-4
更新日期：2019-03-01 00:00:00
abstract：:Multidrug-resistant uropathogenic Escherichia coli (UPEC) is increasing gradually on a worldwide scale. We therefore examined the possibility of bacteriophage (phage) therapy for urinary tract infections (UTIs) caused by the UPEC strains as an alternative to chemotherapy. In addition to the well-known T4 phage, KEP10,...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0031-4
更新日期：2008-01-01 00:00:00
