Abstract：
:Four distinct double-stranded (ds) RNA bands were extracted from leaves of Raphanus sativus-root cv. Yidianhong [corrected] with yellowing at the leaf edge in China. Purified viral particles of 28-30 nm in diameter contained dsRNA segments with the same number and mobility as these extracted directly from radish leaves. The two major dsRNA segments, namely RasR 1 and RasR 2, were 1866 and 1791 bp in length, respectively. Computer analysis predicted that they both contained a single open reading frame (ORF) on their plus-stranded RNA, putatively encoding a RNA dependent RNA polymerase and a capsid protein similar to that encoded by members of the family Partitiviridae. In addition, both RasR 1 and RasR 2 were highly conserved at the 5' untranslated regions (UTR) and had an adenosine-uracil rich stretch at the 3' UTR, with an identical terminal motif (5'-AAAAUAAAACC-3'). Taken together, these results suggest that the two major dsRNA segments constitute the genome of a partitivirus infecting radish.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Chen L,Chen JS,Liu L,Yu X,Yu S,Fu TZ,Liu WHdoi
10.1007/s00705-005-0685-8keywords:
subject
Has Abstractpub_date
2006-05-01 00:00:00pages
849-59issue
5eissn
0304-8608issn
1432-8798journal_volume
151pub_type
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