Identification and characterization of an isolate of apple green crinkle associated virus involved in a severe disease of quince (Cydonia oblonga, Mill.).

Abstract:

:A quince tree showing severe symptoms of a previously undescribed viral disease occurring in northern Apulia (Italy) was analysed using high-throughput sequencing of small RNA libraries, leading to the identification of a new strain of apple green crinkle associated virus (isolate AGCaV-CYD) showing peculiar traits. RT-PCR with specific primers detected AGCaV-CYD in consistent association with symptoms in the surveyed orchards. Molecular characterization of the reconstructed genome, together with phylogenetic analysis, showed it to be closely related to an AGCaV strain causing green crinkle disease in apple (AGCaV-AUR) and divergent from the type strain of apple stem pitting virus (ASPV-PA66).

journal_name

Arch Virol

journal_title

Archives of virology

authors

Morelli M,Giampetruzzi A,Laghezza L,Catalano L,Savino VN,Saldarelli P

doi

10.1007/s00705-016-3074-6

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

299-306

issue

1

eissn

0304-8608

issn

1432-8798

pii

10.1007/s00705-016-3074-6

journal_volume

162

pub_type

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