Abstract:
:A quince tree showing severe symptoms of a previously undescribed viral disease occurring in northern Apulia (Italy) was analysed using high-throughput sequencing of small RNA libraries, leading to the identification of a new strain of apple green crinkle associated virus (isolate AGCaV-CYD) showing peculiar traits. RT-PCR with specific primers detected AGCaV-CYD in consistent association with symptoms in the surveyed orchards. Molecular characterization of the reconstructed genome, together with phylogenetic analysis, showed it to be closely related to an AGCaV strain causing green crinkle disease in apple (AGCaV-AUR) and divergent from the type strain of apple stem pitting virus (ASPV-PA66).
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Morelli M,Giampetruzzi A,Laghezza L,Catalano L,Savino VN,Saldarelli Pdoi
10.1007/s00705-016-3074-6subject
Has Abstractpub_date
2017-01-01 00:00:00pages
299-306issue
1eissn
0304-8608issn
1432-8798pii
10.1007/s00705-016-3074-6journal_volume
162pub_type
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