Abstract：
:The polypeptides associated with fowlpox virus (FPV) infection of chicken embryo skin (CES) cells were examined by metabolic labelling with [35S]-methionine and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Polypeptide synthesis was followed over the first 48 hours post infection, as this was shown to be the period of viable virus production in CES cells. In contrast to infection with vaccinia virus (VV), which leads to a rapid total inhibition of host polypeptide synthesis in a number of cell lines, FPV infection of CES cells failed to cause a complete shut down of host polypeptide synthesis, with only a small number of host polypeptides being inhibited. A total of 21 FPV coded or induced polypeptides were resolved by metabolic labelling. As with VV, these polypeptides can be divided into two groups, the pre-replicative polypeptides containing a single member of 70,000 daltons, synthesised before viral DNA replication, and the post-replicative polypeptides, synthesised only after viral DNA replication has commenced. FPV DNA replication was shown to commence between 12 and 16 hours post-infection and to continue up to 48 hours post-infection. As also observed with VV, two temporally distinct classes of post-replicative polypeptides were identified based on their time of synthesis post-infection. The examination of purified FPV and VV by SDS-PAGE and coomassie blue staining allowed the resolution of 57 FPV particle associated polypeptides and 27 VV associated polypeptides.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Prideaux CT,Boyle DBdoi
10.1007/BF01320959subject
Has Abstractpub_date
1987-01-01 00:00:00pages
185-99issue
3-4eissn
0304-8608issn
1432-8798journal_volume
96pub_type
杂志文章abstract：:Transgenic mice were produced by microinjection of a partial hepatitis C virus (HCV) genome sequence including the structural protein region, under the control of the albumin promoter and enhancer into fertilized eggs of C57BL/6 and BDF1 mice. Three founders carrying at least five copies of the transgene but not expre...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718169
更新日期：1996-01-01 00:00:00
abstract：:Amino acid positions recognized by monoclonal antibodies (MAbs) in the influenza A virus nucleoprotein (NP) have been reported. As these residues were scattered in the three-dimensional (3D) structure of NP, no patterns of the architecture of antibody-binding sites could be inferred. Here, we used site-specific mutage...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1952-8
更新日期：2014-06-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-010-0600-9
更新日期：2010-04-01 00:00:00
abstract：:A method for purification of Hog Cholera Virus (HCV) is presented. Cell-associated virus was extracted from PK15 cells 17 hours p.i. by fluorocarbon treatment. The virus was concentrated by polyethylene glycol precipitation and partially purified by pelleting on a fluorocarbon cushion. Final purification was achieved ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314377
更新日期：1977-01-01 00:00:00
abstract：:Molecular epidemiology can be used to identify human immunodeficiency virus (HIV) transmission clusters, usually using pol sequence for analysis. In the present study, we explored appropriate parameters to construct a simple network using HIV env and gag sequences instead of pol sequences for constructing a phylogenet...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3485-z
更新日期：2017-11-01 00:00:00
abstract：:In order to investigate the genetic diversity of porcine circovirus type 2 (PCV2), 284 clinical tissue samples were collected from different pig farms in central China from 2015 to 2017. A total of 162 tissue samples (162/284, 57.04%) were positive for PCV2 by PCR, and subsequently, the complete genome of 36 of these ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04205-0
更新日期：2019-05-01 00:00:00
abstract：:We studied 67 hepatitis C virus (HCV) isolates from 64 hospitalized patients in Shanghai, China. Genotype 1 was prevalent, and genotypes 2, 3, 6 were found for the first time in Shanghai. A rare mixed infection with three subtypes (1a, 1b, 2a) was found. The complete genome sequence of a subtype 3b isolate was determi...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1457-x
更新日期：2013-01-01 00:00:00
abstract：:Allexiviruses are economically important garlic viruses that are involved in garlic mosaic diseases. In this study, we characterized the allexivirus cysteine-rich protein (CRP) gene located just downstream of the coat protein (CP) gene in the viral genome. We determined the nucleotide sequences of the CP and CRP genes...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3749-2
更新日期：2018-06-01 00:00:00
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journal_title：Archives of virology
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doi：10.1007/s00705-014-2291-0
更新日期：2015-04-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317928
更新日期：1989-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0844-0
更新日期：2002-09-01 00:00:00
abstract：:We have developed an in vitro transcriptional assay using Escherichia coli RNA polymerase to initiate the replication of peach latent mosaic viroid (PLMVd). Regardless of the polarity of the PLMVd strand used as template, initiation in vitro occurred at the same hairpin structure. These initiation sites correspond to ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170061
更新日期：2001-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0178-7
更新日期：2008-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309892
更新日期：1986-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3509-8
更新日期：2017-11-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0533-3
更新日期：2009-01-01 00:00:00
abstract：:Genetic relationships between the genome segments of six cypovirus (CPV) isolates were analysed by RNA cross-hybridisation. These included three type 1 viruses and single isolates of types 2, 5 and 12, which collectively are identical to those previously compared by serology and electrophoresis [Mertens et al. (1989),...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050525
更新日期：1999-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050141
更新日期：1997-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0222-7
更新日期：2008-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2211-3
更新日期：2014-12-01 00:00:00
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doi：10.1007/s00705-003-0112-y
更新日期：2003-07-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311358
更新日期：1989-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00705-020-04763-8
更新日期：2020-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00705-013-1939-5
更新日期：2014-06-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2526-8
更新日期：2015-10-01 00:00:00
abstract：:Cross hemagglutination inhibition (HI) and neuraminidase inhibition (NI) tests demonstrated strong antigenic relationship between A/Swine/Wisconsin/1/73 (SW/73) and A/Swine/Shope/15/31 (SW/31) influenza viruses. An eigthyone fold purification of virus was achieved by adsorption and elution followed by differential ult...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317967
更新日期：1975-01-01 00:00:00
abstract：:At necropsy, several dogs which died showing symptoms of hemorrhagic diarrhea, had significant lesions of the mucosa that were found especially in the duodenum and upper part of the small bowel. Study of ultrathin sections from the diseased mucosa revealed particles resembling parvoviruses in altered nuclei of cells o...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320586
更新日期：1979-01-01 00:00:00
abstract：:Mentha x gracilis 'Variegata', an ornamental mint clone first described about 200 years ago, exhibits virus-like vein banding symptoms. Double-stranded RNA and virion isolations revealed the presence of three viruses in a 'Variegata' plant. Cloning and sequencing disclosed that one of the viruses was a previously unid...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0586-x
更新日期：2006-01-01 00:00:00
abstract：:Sixteen patients with symptoms typical for Ockelbo disease (rash, arthralgia, fever) were enrolled in a 2 1/2 year study, during which clinical symptoms were recorded and ELISA was employed to study specific IgM, IgG and IgG subclass development. Initially, all patients presented with rash and arthralgia, and five pat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01379107
更新日期：1994-01-01 00:00:00
abstract：:Konjak mosaic virus (KoMV) belongs to the genus Potyvirus, family Potyviridae. The complete nucleotide sequence of KoMV F isolate (KoMV F) was determined. The genome is 9,544 nucleotides long excluding the 3' terminal poly A tail and encodes a typical potyviral 350-kDa polyprotein of 3,087 amino acids. Phylogenetic an...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0735-x
更新日期：2006-08-01 00:00:00
