Abstract:
:Use of Urografin and Conray for the equilibrium centrifugation of viruses is described. These pharmaceuticals, which consist of iodinated arylic compounds, reach densities of 1.6 g/cm3 and have low intrinsic viscosities. Poliovirus, Newcastle disease virus, and lymphocytic choriomeningitis virus were centrifuged to equilibrium in gradients made of these substances. Viral infectivities were not measurably affected, which is especially noteworthy in the case of the very labile lymphocytic choriomeningitis virus. Buoyant densities were found to be significantly lower than densities obtained with gradients made of CsCl and sucrose.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Gschwender HH,Rutter G,Popescu Mdoi
10.1007/BF01318245subject
Has Abstractpub_date
1975-01-01 00:00:00pages
359-64issue
4eissn
0304-8608issn
1432-8798journal_volume
49pub_type
杂志文章abstract::Hepatitis E virus (HEV) genotype 3 is an emerging pathogen in the developed world. As the clinical manifestations and routine laboratory parameters are often nonspecific, accurate diagnostic tests are crucial. In the current study, the performance of six serological assays and three PCR assays for the detection of HEV...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3395-0
更新日期:2017-09-01 00:00:00
abstract::An activator protein, Alt, synthesized during the early state of lytic infection is required for transcription of the late operon in the lactococcal phage TP901-1. In order to identify amino acid residues in the Alt protein required for activation of the TP901-1 late promoter, P(late), hydroxylamine mutagenesis was pe...
journal_title:Archives of virology
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doi:10.1007/s00705-006-0851-7
更新日期:2007-02-01 00:00:00
abstract::Scrapie and Creutzfeldt-Jakob disease (CJD) are caused by infectious agents that are defined phenomenologically. No agent-specific molecules or particles have been identified. Biological properties, such as exponential agent replication and strain variation, as well as physical characteristics of infectivity indicate ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309662
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0419-4
更新日期:2009-01-01 00:00:00
abstract::Mitochondrial movement and distribution throughout the cytoplasm is crucial for maintaining cell homeostasis. Mitochondria are dynamic organelles but can be functionally disrupted during infection. Here, we show that the ubiquitous human pathogens HHV-1 and HHV-2 induce changes in the mitochondrial morphology and dist...
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pub_type: 杂志文章
doi:10.1007/s00705-018-3890-y
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abstract::The interaction between the nucleocapsid (N) protein of mouse hepatitis virus (MHV) and RNA was studied in an effort to define portions of the N molecule that participate in binding to RNA. N mRNAs transcribed from SP6 and T7 vectors were translated in a rabbit reticulocyte lysate. Analysis of synthesized N protein in...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309634
更新日期:1992-01-01 00:00:00
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journal_title:Archives of virology
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更新日期:2013-10-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0143-4
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050131
更新日期:1997-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0330-y
更新日期:2004-10-01 00:00:00
abstract::In the present study, the detection of a pantropic canine coronavirus (CCoV) strain in a dog with lethal diarrhoea is reported. RT-PCR and real-time RT-PCR assays were used for the detection, characterization and quantitation of CCoV. Sequence and phylogenetic analysis of the CCoV NA/09 revealed a high degree of seque...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-1141-6
更新日期:2012-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2999-0
更新日期:2016-11-01 00:00:00
abstract::Feline calicivirus (FCV) is a highly contagious pathogen that causes oral and upper respiratory tract disease in cats. Despite widespread vaccination, the prevalence of FCV remains high. Furthermore, a high gene mutation rate has led to the emergence of variants, and some infections are lethal. To date, there is no ef...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2534-8
更新日期:2015-12-01 00:00:00
abstract::Infection with West Nile virus and dengue virus, two mosquito-borne flaviviruses, is enhanced by two calcium-dependent lectins: dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN), and its related molecule (DC-SIGNR). The present study examined the relationship between Japanese enc...
journal_title:Archives of virology
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doi:10.1007/s00705-014-2042-2
更新日期:2014-08-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0777-y
更新日期:2010-11-01 00:00:00
abstract::A quasi-spherical virus was isolated from a cultivated Amazon lily plant (Eucharis grandiflora) that could be mechanically transmitted to healthy E. grandiflora plants, subsequently producing mild mosaic or mottle symptoms on the leaves. The purified virus consisted of three quasi-spherical particles about 20 nm wide ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1467-8
更新日期:2013-01-01 00:00:00
abstract::Little is known about the bacteriophage proteins expressed immediately after infection of the host cell. Most of these early proteins are probably involved in bacteriophage-host interactions redirecting the bacterial metabolism to phage production. Interaction analysis of the first 16 phiKMV gene products (gp) identif...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-007-0967-4
更新日期:2007-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718322
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abstract::Infectivity of non-occluded particles of nuclear polyhedrosis virus infecting Galleria mellonella L. was determined with bioassay technique in vivo and with plaque assay method in vitro using SCLd 135 (Quiot) established cell culture. A plaque-forming virus unit corresponds to about 50 ID50 for Galleria larvae injecte...
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pub_type: 杂志文章
doi:10.1007/BF01315023
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050044
更新日期:2000-01-01 00:00:00
abstract::A novel iflavirus, tentatively named "Sogatella furcifera honeydew virus 1" (SFHV1), discovered through transcriptome sequencing analysis of white-backed planthoppers (Sogatella furcifera) collected in southern China, is described here. The full genome of SFHV1 is 10,837 nucleotides (nt) long, including the polyA tail...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3817-7
更新日期:2018-07-01 00:00:00
abstract::Out of 136 new phages, 80 (59%) are classified into 23 species according to morphology and physicochemical properties. Six new species are described and species beta 4, from a previous classification scheme, is renamed T1. The morphology of 36 phage species is schematically represented. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050170095
更新日期:2001-07-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01311183
更新日期:1994-01-01 00:00:00
abstract::Retrovirus infectivity is the result of a cooperative interaction of three structural genes, gag, pol, and env. Since the L-cell endogenous retrovirus (LCV) lacks the env gene translation product, our aim was to study the biological and molecular basis of its non-infectiousness. Fusion experiments between LCV and homo...
journal_title:Archives of virology
pub_type: 杂志文章
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-005-0637-3
更新日期:2006-01-01 00:00:00
abstract::A viroid was isolated from symptomless Coleus blumei cultivar (cv) 'Fairway Ruby' plants using the bidirectional PAGE method for analysis of small circular RNA molecules. The viroid was transmitted to viroid-free plants of Coleus blumei cv. 'Scarlet Dragonfly' by mechanical inoculation. Cloning and sequencing revealed...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718241
更新日期:1996-01-01 00:00:00
abstract::The stools of slaughtered pigs were screened for hepatitis E virus (HEV). HEV RNA was detected in 7.3% of the samples. HEV strains were characterized as genotype 3 subtype c, a cluster previously not described in Italy. These findings provide evidence that slaughterhouse workers may be exposed to HEV infection. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0544-0
更新日期:2010-01-01 00:00:00