Use of iodinated organic compounds for the density gradient centrifugation of viruses.

Abstract:

:Use of Urografin and Conray for the equilibrium centrifugation of viruses is described. These pharmaceuticals, which consist of iodinated arylic compounds, reach densities of 1.6 g/cm3 and have low intrinsic viscosities. Poliovirus, Newcastle disease virus, and lymphocytic choriomeningitis virus were centrifuged to equilibrium in gradients made of these substances. Viral infectivities were not measurably affected, which is especially noteworthy in the case of the very labile lymphocytic choriomeningitis virus. Buoyant densities were found to be significantly lower than densities obtained with gradients made of CsCl and sucrose.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Gschwender HH,Rutter G,Popescu M

doi

10.1007/BF01318245

subject

Has Abstract

pub_date

1975-01-01 00:00:00

pages

359-64

issue

4

eissn

0304-8608

issn

1432-8798

journal_volume

49

pub_type

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