Abstract：
:An activator protein, Alt, synthesized during the early state of lytic infection is required for transcription of the late operon in the lactococcal phage TP901-1. In order to identify amino acid residues in the Alt protein required for activation of the TP901-1 late promoter, P(late), hydroxylamine mutagenesis was performed, resulting in almost saturating mutagenesis of alt. Twenty-three different non-functional alt alleles containing one, and in one case two amino acid exchanges were isolated and analyzed. Eight of the twenty-three mutant proteins were still able to activate the P(late) promoter to some extent. Our results show that alt encodes a protein of 16.7 kDa and that the last fourteen amino acids in the C-terminal part of the protein are required for activation of the P(late) promoter. By combining sequence analysis with experimental data we suggest that the C-terminal half of the Alt protein contains a helix-turn-helix-like motif involved in DNA binding. We also propose that the C-terminal half of the Alt protein may be involved in interactions with the bacterial RNA polymerase, whereas the N-terminal half of the protein is proposed to be important for the overall protein structure.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Pedersen M,Hammer Kdoi
10.1007/s00705-006-0851-7subject
Has Abstractpub_date
2007-02-01 00:00:00pages
305-20issue
2eissn
0304-8608issn
1432-8798journal_volume
152pub_type
杂志文章abstract：:A factor secreted from avian cells infected non productively with a non cytopathogenic mutant of vesicular stomatitis virus (VSV ts 1026) interferes with HIV replication in CEM cells and peripheral blood monocytes (PBL). Production of infectious particles is decreased and many virions lack cores and/or spikes. In CEM ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317266
更新日期：1992-01-01 00:00:00
abstract：:Semliki Forst Virus (SFV) infected chick embroyo cells (CEC) incubated in the presence of Tunicamyein, a drug which specifically inhibits glycosylation, were studied under the electron microscope. No extracellular virions were produced although intracellular viral nucleocapsids in paracrystalline form were detected. U...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314489
更新日期：1977-01-01 00:00:00
abstract：:Blue-eye disease is an emergent viral swine infection caused by porcine rubulavirus (PoRV). We have developed a qRT-PCR method to detect and quantify expression of the nucleoprotein gene for different PoRV strains. The limit of detection for this assay was 10(2) copies of synthetic RNA. Viral RNA from PoRV was detecta...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1672-0
更新日期：2013-09-01 00:00:00
abstract：:Changes in the NP gene of H5N1 highly pathogenic avian influenza (HPAI) viruses have previously been shown to affect viral replication, alter host gene expression levels and affect mean death times in infected chickens. Five amino acids at positions 22, 184, 400, 406, and 423 were different between the two recombinant...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0399-4
更新日期：2009-01-01 00:00:00
abstract：:Ultraviolet (UV) inactivation and photoreactivation of Anacystis nidulans and cyanophage AS-1 was studied at different wavelengths. UV inactivation of free phage particles and one and two hour host-phage complexes (intracellular phages) were exponential. UV resistance of plaque forming units was attained at the latter...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317413
更新日期：1979-01-01 00:00:00
abstract：:Rice transitory yellowing virus (RTYV), a member of the genus Nucleorhabdovirus, is closely related to or synonymous with rice yellow stunt virus (RYSV). To clarify the relationship between RTYV and RYSV, we determined the nucleotide sequence of the RTYV genome. The RTYV genome consists of 14,029 nucleotides. The over...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0557-8
更新日期：2010-02-01 00:00:00
abstract：:Two and a half hours after infection with a high dose of different strains of HSV-1 which induce rounding of cells, breakdown of actin containing microfilaments can be observed. At the periphery of the cell, actin containing knob-like protuberances were visible. Later on, actin seems to be located exclusively on the s...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314285
更新日期：1986-01-01 00:00:00
abstract：:We have investigated whether poly(I:C) Toll-like receptor 3 (TLR3) and resiquimod Toll-like receptor 7 (TLR7) agonists can serve as vaccine adjuvants and promote the efficiency of therapeutic DNA vaccination against tumors expressing the human papilloma virus 16 (HPV-16) E7 protein. For this purpose, C57BL/6 mice were...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2024-4
更新日期：2014-08-01 00:00:00
abstract：:Adenoviruses are common pathogens that are responsible for a wide variety of infectious syndromes. The objectives of this study were to identify and characterize members of different adenovirus species at the molecular level and to describe the correlation between viruses and clinical syndromes during a period of 4 ye...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0338-4
更新日期：2009-01-01 00:00:00
abstract：:Two double-stranded RNA (dsRNA) mycoviruses were found in isolate QSP5 of the rice blast fungus Magnaporthe oryzae. Sequence analysis of the two dsRNA mycoviruses revealed that one is closely related to Magnaporthe oryzae virus 2 (MoV2), and the other one is related to Magnaporthe oryzae chrysovirus 1-A (MoCV1-A). The...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2562-4
更新日期：2015-11-01 00:00:00
abstract：:In this study, we isolated a mycobacteriophage infecting Mycobacterium smegmatis mc2155 from a soil sample collected in Shandong Province in China. This phage was named Shandong1. It is a member of the family Siphoviridae with an isometric head and a long tail. Its genome was found to be 60,618 bp long with 67.46% G +...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3534-7
更新日期：2017-12-01 00:00:00
abstract：:In this study, a novel mycovirus designed Colletotrichum gloeosporioides ourmia-like virus 1 (CgOLV1) was isolated from a filamentous phytopathogenic fungus, Colletotrichum gloeosporioides. The virus has a genome of 2,516 nucleotides and contains a large open reading frame (ORF) encoding an RNA-dependent RNA polymeras...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04346-2
更新日期：2019-10-01 00:00:00
abstract：:Establishment of a persistent infection of Spodoptera frugiperda nuclear polyhedrosis virus (NPV) in Spodoptera frugiperda (S.f.) cells occurred in three phases: the first phase was characterised by high levels of cell infection and death, the second phase by decreasing cell infection levels leading to the final phase...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310859
更新日期：1983-01-01 00:00:00
abstract：:Nanoviruses are multi-component plant-infecting single-stranded DNA viruses. Using a viral metagenomics-informed approach, a new nanovirus and two associated alphasatellite molecules have been identified in an uncultivated asymptomatic Vicia cracca plant in the Rhône region of France. This novel nanovirus genome inclu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3634-4
更新日期：2018-03-01 00:00:00
abstract：:A collection of rotavirus samples collected over a 26-year period was examined to study the dynamics of change in RV strains of genotype P[8] in a geographically defined population (Nizhny Novgorod, Russia; children under 6 years) with no vaccine pressure. Phylogenetic analysis of gene VP4 (subunit VP8*) showed the pr...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1426-4
更新日期：2012-12-01 00:00:00
abstract：:Glycoprotein D (gD) of bovine herpesvirus 1 (BHV-1), a homolog of herpes simplex virus gD, represents a major component of the viral envelope and is a dominant immunogen. To study the antigenic properties of the different regions of gD, we have expressed the full-length gD encoding gene and overlapping fragments spann...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050450
更新日期：1998-01-01 00:00:00
abstract：:Porcine respiratory coronavirus (PRCV), a spike (S) gene deletion mutant of Transmissible gastroenteritis virus (TGEV), causes mild or subclinical respiratory infections in pigs. The shedding of PRCV/TGEV was studied at different days post-arrival in fecal and nasal swabs from PRCV/TGEV seronegative sentinel pigs intr...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0245-z
更新日期：2004-05-01 00:00:00
abstract：:Optimal conditions of radial haemolysis in gel (HIG) with Chikungunya (Chik) and Japanese encephalitis (JE) viruses were developed and compared with those of rubella virus. Sheep erythrocytes were found preferable for HIG with Chik and JE viruses, unlike rubella virus, where goose erythrocytes gave best results. Roost...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320619
更新日期：1980-01-01 00:00:00
abstract：:Both H9N2 subtype avian influenza and 2009 pandemic H1N1 viruses (pH1N1) can infect humans and pigs, which provides the opportunity for virus reassortment, leading to the genesis of new strains with potential pandemic risk. In this study, we generated six reassortant H9 viruses in the background of three pH1N1 strains...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2009-3
更新日期：2014-07-01 00:00:00
abstract：:Since the first rescue of a recombinant Newcastle disease virus (rNDV) in the late 1990s, many more rNDVs have been rescued by researchers around the world. Regardless of methodology, the main principle behind rescue of the virus has remained the same, i.e., the formation of a functional replication complex by simulta...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-016-3065-7
更新日期：2017-01-01 00:00:00
abstract：:A recombinant encephalomyocarditis virus (rEMCV2887A-egfp) expressing the enhanced green fluorescent protein (EGFP) was produced. The EGFP gene was inserted in frame within the leader protein coding sequence of a full-length cDNA clone of EMCV. RNA transcripts derived from the recombinant full-length cDNA were synthes...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0746-7
更新日期：2006-09-01 00:00:00
abstract：:Bovine leukemia virus (BLV) is a member of the genus Deltaretrovirus of the family Retroviridae and cause a chronic lymphosarcoma, which is extensive in cattle. In yaks (Bos grunniens), the distribution, strains and genetic characteristics of BLV have rarely been studied. The aim of our study was to investigate BLV in...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3658-9
更新日期：2018-03-01 00:00:00
abstract：:Human respiratory syncytial virus (RSV) is recognized as a leading pathogen responsible for severe respiratory infections in the pediatric population, particularly in infants and young children. A previous study by the same study group revealed that the RSV BA strain was prevalent in southwestern China in epidemic sea...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1552-z
更新日期：2013-07-01 00:00:00
abstract：:Concentrations of DEAE-dextran which are currently used to stimulate the interaction between animal cells and natural or synthetic polynucleotides, inhibit protein synthesis in HeLa cells. This inhibition results from the blocking of more than one of the steps in protein synthesis, including transport of amino acids. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF02175599
更新日期：1975-01-01 00:00:00
abstract：:A new virus belonging to the family Dicistroviridae was identified in the hibiscus-infesting cotton mealybug Phenacoccus solenopsis. Using high-throughput sequencing (HTS) on an Illumina HiSeq platform, a single contig of the complete genome sequence was assembled. The authenticity of the sequence obtained by HTS was ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04702-7
更新日期：2020-09-01 00:00:00
abstract：:Outbreaks of swine influenza were first observed in Japan in 1978. A number of influenza viruses were isolated from diseased swine. Almost all viruses isolated were swine influenza virus (Hsw 1 N1) but two viruses isolated from the nasal swabs of swine showing clinical signs of influenza in the Kanagawa prefecture wer...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314741
更新日期：1980-01-01 00:00:00
abstract：:A novel Vibrio alginolyticus phage, VAP7, was isolated from seawater collected from Sanya, Hainan province, China. Whole-genome sequencing analysis revealed that phage VAP7 has a linear, double-stranded DNA genome of 144,685 bp with an average G+C content of 41.9% and a high degree of sequence similarity to Vibrio pha...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04535-4
更新日期：2020-04-01 00:00:00
abstract：:Amino acids Lys34, His36, and Phe37 were substituted by PCR-mediated, site-directed mutagenesis for three Trp's in the AcNPV polyhedrin sequence. Phase contrast microscopy revealed refringent, amorphous polyhedra in the nuclei of infected cells. Electron microscopy confirmed a great variation in form and size of the m...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050675
更新日期：2000-01-01 00:00:00
abstract：:A novel iflavirus, tentatively named "Sogatella furcifera honeydew virus 1" (SFHV1), discovered through transcriptome sequencing analysis of white-backed planthoppers (Sogatella furcifera) collected in southern China, is described here. The full genome of SFHV1 is 10,837 nucleotides (nt) long, including the polyA tail...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3817-7
更新日期：2018-07-01 00:00:00
abstract：:Newborn WKA rats given a single intraperitoneal injection of MMC treated TARS-1, a rat T cell line producing HTLV-I, were shown to accumulate high titred antibodies specific for HTLV-I. Adult WKA rats rejected TARS-1 transplant with transient appearance of anti-HTLV-I antibodies. However, rats maintained under daily a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314420
更新日期：1987-01-01 00:00:00
