Characteristics and complete genome sequence of the virulent Vibrio alginolyticus phage VAP7, isolated in Hainan, China.

Abstract:

:A novel Vibrio alginolyticus phage, VAP7, was isolated from seawater collected from Sanya, Hainan province, China. Whole-genome sequencing analysis revealed that phage VAP7 has a linear, double-stranded DNA genome of 144,685 bp with an average G+C content of 41.9% and a high degree of sequence similarity to Vibrio phage VP-1. Annotation results identified 193 open reading frames and one transfer RNA-encoding gene in the phage genome. The morphology and the results of phylogenetic analysis suggest that VAP7 should be classified as a new member of the family Ackermannviridae. Moreover, phage VAP7 grew over a wide pH (5.0-10.0) and temperature (4-40 °C) range. Host-range experiments revealed that VAP7 could infect 31 Vibrio alginolyticus strains. Thus, VAP7 infecting Vibrio alginolyticus strains represents a potential new candidate for use in phage therapy.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Gao M,Qin Y,Fan H,Zhang X,Li P,Liu H,Pei G,Mi Z,Xu X,Tong Y,Bai C

doi

10.1007/s00705-020-04535-4

subject

Has Abstract

pub_date

2020-04-01 00:00:00

pages

947-953

issue

4

eissn

0304-8608

issn

1432-8798

pii

10.1007/s00705-020-04535-4

journal_volume

165

pub_type

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