Abstract：
:Two and a half hours after infection with a high dose of different strains of HSV-1 which induce rounding of cells, breakdown of actin containing microfilaments can be observed. At the periphery of the cell, actin containing knob-like protuberances were visible. Later on, actin seems to be located exclusively on the surface of cells. Observations were done by immunofluorescence microscopy, scanning electron-microscopy and immunoperoxidase staining of ultrathin sections. The envelope of HSV appears to be stained by anti-actin. Strain IES produces rounding of cells at a high dose of infection before fusion proceeds at 37 degrees C. Similar alterations were not observed with the fusing strains MP and HFEM. Incubation of infected cells at 39 degrees C revealed strain dependent differences of the fusion activity. At 41 degrees C no "fusion from within" of cells but only rounding was detectable. Application of tunicamycin resulted in complete inhibition of fusion by all strains. The fusion activity of some strains of HSV-1 (ANG, HFEM, and MP) was not inhibited by addition of 2-deoxy-D-glucose and 2-fluoro-deoxy-D-glucose. A variant from strain MP could be isolated, which is sensitive to the effects of 2-deoxy-D-glucose. Inhibitors of processing of glycoproteins did not affect fusion of cells.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Heeg U,Dienes HP,Müller S,Falke Ddoi
10.1007/BF01314285subject
Has Abstractpub_date
1986-01-01 00:00:00pages
257-70issue
3-4eissn
0304-8608issn
1432-8798journal_volume
91pub_type
杂志文章abstract：:Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) are two closely related alphaherpesviruses. BoHV-1 causes several syndromes in cattle, including respiratory disease and sporadic cases of encephalitis, whereas BoHV-5 is responsible for meningoencephalitis in calves. Although both viruses are neurotropic, they differ...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-019-04494-5
更新日期：2020-02-01 00:00:00
abstract：:We constructed a recombinant baculovirus encoding a dengue (DEN)-2 virus envelope glycoprotein truncated of 102 amino acids (aa) at its C-terminus (D2E delta 102). The production, processing and transportation of the recombinant protein in baculovirus-infected Spodoptera frugiperda (Sf9) cells and its immunogenic prop...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309465
更新日期：1994-01-01 00:00:00
abstract：:In previous work, we demonstrated that the arenavirus Junín virus (JUNV) is able to activate Akt by means of the phosphatidylinositol-3-kinase (PI3K) survival pathway during virus entry. This work extends our study, emphasizing the relevance of this pathway in the establishment and maintenance of persistent infection ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2298-6
更新日期：2015-02-01 00:00:00
abstract：:Cocksfoot mottle sobemovirus (CfMV) has a monopartite single-stranded positive-sense RNA genome. In wheat germ extract, in vitro translation of CfMV virion RNA resulted in the production of four major proteins of 100, 71, 34 and 12 kDa. In this paper we show the individual genes from which these polypeptides are synth...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050610
更新日期：1999-01-01 00:00:00
abstract：:The isolation of ortho- and paramyxovirus glycoproteins using a new nonionic detergent (MESK) is reported. MESK was shown to solubilize most of the viral envelope glycoproteins without decreasing their biologic activity. Solubilized glycoproteins are not contaminated by any internal viral proteins or by appreciable qu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314237
更新日期：1985-01-01 00:00:00
abstract：:The present study on genetic diversity of human papillomaviruses in women infected by HIV in Brazil describes the frequency, the genotypes, and five new variants of HPV. One hundred fifty cervical smears of HIV-positive women were subjected to cytological examination, and the DNA samples obtained were assayed by MY09/...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0828-6
更新日期：2007-01-01 00:00:00
abstract：:We report the complete genome sequencing of two Escherichia coli T5-related bacteriophages, DT57C and DT571/2, isolated from the same specimen of horse feces. These two isolates share 96% nucleotide sequence identity and can thus be considered representatives of the same novel species within the genus T5likevirus. The...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2582-0
更新日期：2015-12-01 00:00:00
abstract：:The present study confirms that herpesviruses (LF) previously isolated from testes and buffy coat cells of male turkeys with semen abnormalities establish a latent infection in testicular cells. These experiments also present the first evidence that these herpesviruses are harbored latently in cells of spinal ganglia....
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311163
更新日期：1984-01-01 00:00:00
abstract：:The sequence variability of Barley yellow dwarf virus-PAV (PAV) and Barley yellow dwarf virus-MAV (MAV) was studied by comparing 502 nucleotides from the coat protein-encoding region of six isolates from Latvia and four from Sweden. The diversity within MAV was low (>97% sequence identity), also when compared to isola...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0242-2
更新日期：2004-04-01 00:00:00
abstract：:Monitoring of influenza A virus (IAV) was conducted in wild bird species in central Thailand. Four IAV subtype H12N1 strains were isolated from a watercock (order Gruiformes, family Rallidae) (n = 1) and lesser whistling ducks (order Anseriformes, family Anatidae) (n = 3). All H12N1 viruses were characterized by whole...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1260-8
更新日期：2012-06-01 00:00:00
abstract：:The gene encoding the capsid protein of a genogroup I Norwalk-like virus (NLV) (Hu/NLV/Stav/95/Nor) was cloned and expressed in insect cells using a baculovirus vector. The His-tagged recombinant capsid protein (rStav) was antigenic and immunogenic, showed an apparent molecular weight of approximately 68 kD in protein...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050665
更新日期：2000-01-01 00:00:00
abstract：:Gp 85 the major envelope glycoprotein of Rous sarcoma virus can be released from intact by virus treatment with 2-mercaptoethanol. Attempts were made to reassociate released gp 85 to 2-mercaptoethanol treated virus. In order to study the biological activity and to analyse the reaction product with biochemical methods ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314883
更新日期：1982-01-01 00:00:00
abstract：:A cross-sectional study was undertaken during 2012-2013 to determine the prevalence, strains and factors associated with rotavirus infection among under-5-year-old children hospitalized with acute diarrhea in Uganda. Rotaviruses were detected in 37 % (263/712) of the children. The most prevalent strains were G9P[8] (2...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2742-2
更新日期：2016-04-01 00:00:00
abstract：:The nucleotide sequences of the entire F genes of two isolates of the pigeon PMV-1 (PPMV-1) variant of Newcastle disease virus (NDV) were determined using RTPCR. The deduced amino acid sequences of the F0 protein showed four differences between isolate 760/83 which had been passaged 4 times in chickens and gave an int...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718322
更新日期：1996-01-01 00:00:00
abstract：:Amino acid positions recognized by monoclonal antibodies (MAbs) in the influenza A virus nucleoprotein (NP) have been reported. As these residues were scattered in the three-dimensional (3D) structure of NP, no patterns of the architecture of antibody-binding sites could be inferred. Here, we used site-specific mutage...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1952-8
更新日期：2014-06-01 00:00:00
abstract：:Molecular variability was assessed for 18 isolates of cowpea mild mottle virus (CPMMV, genus Carlavirus, family Betaflexiviridae) found infecting soybean in various Brazilian states (Bahia, Goiás, Maranhão, Mato Grosso, Minas Gerais, Pará) in 2001 and 2010. A variety of symptoms was expressed in soybean cv. CD206, ran...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1879-0
更新日期：2014-04-01 00:00:00
abstract：:The ability of human fibroblast interferon to suppress colony formation and papovavirus T-antigen expression in six human tumor cell lines did not appear to correlate with their content of chromosomes 16 and 21. Additional factors or chromosomes may be involved in the manifestation of interferon action in aneuploid ce...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317505
更新日期：1979-01-01 00:00:00
abstract：:The effects of relative humidity and temperature on the survival of human rotavirus in a thin layer of faeces on an impervious surface and on absorbent material was investigated using the indirect immunofluorescence technique in LLC-MK2 cells to titrate infectivity. Rotavirus was found to be very stable at low and hig...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01348963
更新日期：1982-01-01 00:00:00
abstract：:The sequence of the gene encoding the membrane protein of human coronavirus 229 E (HCV 229 E) has been determined. The primary translation product, deduced from the DNA sequence, is a polypeptide of 225 amino acids with a predicted molecular weight of 26,000. The polypeptide has 3 potential N-glycosylation sites. Many...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317928
更新日期：1989-01-01 00:00:00
abstract：:The VP1 encoded by genomic segment B of birnaviruses is generally known to exist as a genome-linked protein (VPg) and as a "free" polypeptide of 90 kDa in virus particles. The guanylylation activity associated with infectious bursal disease virus (IBDV) was demonstrated by incubating purified virus in presence of [alp...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050154
更新日期：1997-01-01 00:00:00
abstract：:Gnotobiotic lambs were protected against rotavirus infection by the presence in the gut at the time of infection of colostrum or serum containing antibodies to rotavirus. This protection was observed even when passively-acquired antibody was not present in the serum of the infected lamb. Infection under these conditio...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01348017
更新日期：1976-01-01 00:00:00
abstract：:The effect of temperature on the response of tomato (Lycopersicon esculentum Mill. cv. Rutgers) to infections with the necrogenic cucumber mosaic virus (CMV) satellite D-CARNA 5 was investigated with each of four CMV strains D, 1, Y and S functioning as helper virus. At 24 degrees C lethal necrosis was observed in all...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309724
更新日期：1995-01-01 00:00:00
abstract：:Bubaline alphaherpesvirus 1 (BuHV1) is a member of the family Herpesviridae, subfamily Alphaherpesvirinae, genus Varicellovirus. To date, no full genome sequence of BuHV has been published. Here, we report the complete genome sequence of bubaline alphaherpesvirus 1 (BuHV1) strain b6 (BuHV1-b6), isolated from a water b...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3218-8
更新日期：2017-05-01 00:00:00
abstract：:Intravenous polyoma virus inoculation into pregnant Wistar rats resulted in transplacental infection of the foetus, causing tumours and hydronephroses. Cyclosporin A reduced these effects significantly. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313783
更新日期：1993-01-01 00:00:00
abstract：:Astroviruses are associated with acute gastroenteritis of human and many animal species. Recently, two studies have reported that novel astroviruses were found in bats. In order to extensively understand the genetic and phylogenetic characterization of bat astroviruses, we tested fecal samples of nine bat species that...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1011-2
更新日期：2011-08-01 00:00:00
abstract：:Human cytomegalovirus (HCMV) has evolved several immune-evasion strategies. One strategy involves encoding Fcgamma receptor (FcγR)-like proteins that thwart the Fcγ-mediated effector functions. Our aim was to determine whether GM allotypes-immunoglobulin γ chain determinants expressed primarily on the Fc segment-modul...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-0937-8
更新日期：2011-05-01 00:00:00
abstract：:Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), a disease that has worldwide distribution. Whilst it has been eradicated in most of Western Europe and Scandinavia, it remains a problem in other regions, particularly Eastern Europe and South America. For this study, in 2013, 24 cat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3241-4
更新日期：2017-06-01 00:00:00
abstract：:Unfortunately, the original article was published with incorrect accession number and the correct version is updated here. ...
journal_title：Archives of virology
pub_type： 杂志文章,已发布勘误
doi：10.1007/s00705-019-04326-6
更新日期：2019-09-01 00:00:00
abstract：:To evaluate the variation of Potato mop-top pomovirus from potato fields, 21 isolates were collected from different Danish locations. Reverse transcription-polymerase chain reaction-restriction fragment length polymorphism (RT-PCR-RFLP) of regions of RNA2 was performed for all 21 isolates resulting in the establishmen...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0926-z
更新日期：2003-02-01 00:00:00
abstract：:Infectious cDNA clones of the Shanghai isolate of Ribgrass mosaic virus (RMV) were produced by joining four overlapping cDNA fragments and also in a single step by long template PCR. After inoculation of Nicotiana glutinosa with either RNA transcripts or the cDNA under the control of the CaMV 35S promoter, plants deve...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0740-0
更新日期：2006-09-01 00:00:00
