Abstract:
:The gene encoding the capsid protein of a genogroup I Norwalk-like virus (NLV) (Hu/NLV/Stav/95/Nor) was cloned and expressed in insect cells using a baculovirus vector. The His-tagged recombinant capsid protein (rStav) was antigenic and immunogenic, showed an apparent molecular weight of approximately 68 kD in protein gels, and was only soluble under denaturing conditions. The amino acid sequence of the rStav protein showed 65-88% similarity to capsid protein sequences from other genogroup I NLV and was most closely related to Desert Shield virus. Norwegian recruit sera were tested for antibodies against rStav by Western blotting (rStav WB). The sera had previously been tested for antibodies against a recombinant Norwalk virus capsid protein in an ELISA (rNV ELISA). Several rNV ELISA-negative sera showed a positive response in the rStav WB, indicating that the use of antigens representing different stains may be necessary when screening sera for antibodies against genogroup I NLV.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Myrmel M,Rimstad Edoi
10.1007/s007050050665keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
711-23issue
4eissn
0304-8608issn
1432-8798journal_volume
145pub_type
杂志文章abstract::We describe an unexpected feature observed for the heterologous expression of the Thyrinteina arnobia cypovirus polyhedrin from a recombinant baculovirus infection in different insect cell lines. The in cellulo-formed crystals varied in size and shape depending on the cell line. Crystals formed in Trichoplusia ni-deri...
journal_title:Archives of virology
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更新日期:2003-12-01 00:00:00
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更新日期:2020-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s007050050044
更新日期:2000-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050170048
更新日期:2001-10-01 00:00:00
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abstract::A 945 nucleotide region (bases 76-1020) of the HA1 part of the HA gene was obtained for 31 influenza viruses of H7 subtype isolated primarily from Europe, Asia and Australia over the last 20 years. These were analysed phylogenetically and compared with sequences of the same region from 23 H7 subtype viruses available ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050695
更新日期:2000-01-01 00:00:00
abstract::Group C rotavirus (GCRV) infection has been described in several parts of the world, predominantly as sporadic cases of acute gastroenteritis. Little is known about the yearly changes in the GCRV strains from diarrheal outbreaks. Stool samples collected from outbreaks of acute gastroenteritis in Yokohama, Japan, betwe...
journal_title:Archives of virology
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更新日期:2009-01-01 00:00:00
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更新日期:2002-04-01 00:00:00
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abstract::Beak and feather disease virus (BFDV) is an avian circovirus, and it has a single-stranded DNA genome. It causes a fatal disease in parrots called psittacine beak and feather disease (PBFD). After screening of samples collected from Taiwan using PCR, complete genome sequences of isolates from 21 samples from various s...
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abstract::Hepatitis C virus (HCV) infection causes acute and chronic liver disease and may lead to cirrhosis, liver failure or hepatocellular carcinoma. The behavior of illicit drug users (DUs) typically exposes them to risks of viral infection. In the Brazilian Amazon region, a number of studies have identified high rates of d...
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更新日期:1982-01-01 00:00:00
abstract::Since the appearance of resistance to antiretroviral treatment is unavoidable, the host cell's transcription factor NF-kappaB is a novel HIV target. The goal of this study was to characterize the effect of two immunomodulators, curcumin (Cur) and sulfasalazine (Sul), with a protease inhibitor, indinavir (IDV), on HIV-...
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