Abstract：
:The E5 protein of papillomaviruses is a strongly hydrophobic membrane protein that can associate with the 16 kDa protein subunit of the vacuolar proton ATPase in endosomes and the Golgi apparatus resulting in raise of intraorganelle pH. We demonstrate that E5 of human papillomavirus type 16 (HPV16) when transfected into human keratinocytes localizes to the Golgi. Using FACS analysis and western blotting with a variety of lectins as well as analysing the sialylation status of a specific cell surface glycoprotein CD95 (APO-1/Fas), we show that HPV16 E5 does not grossly affect cellular glycosylation, a main Golgi function.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Oetke C,Auvinen E,Pawlita M,Alonso Adoi
10.1007/s007050070048keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
2183-91issue
10eissn
0304-8608issn
1432-8798journal_volume
145pub_type
杂志文章abstract：:A previously unidentified chicken parvovirus (ChPV) strain, associated with runting-stunting syndrome (RSS), is now endemic among chickens in China. To explore the genetic diversity of ChPV strains, we determined the first complete genome sequence of a novel ChPV isolate (GX-CH-PV-7) identified in chickens in Guang Xi...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2999-0
更新日期：2016-11-01 00:00:00
abstract：:Agarose in situ digestion was used to prepare intact Amsacta moorei entomopoxvirus (AmEPV) DNA from embedded occlusion bodies (OBs). Direct dissolution of OBs in agarose eliminated the necessity for separate purification of virions. A physical map of AmEPV DNA was constructed for five restriction enzymes (BamHI, EcoRI...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310704
更新日期：1990-01-01 00:00:00
abstract：:At present, there are no effective antiviral treatments available for contagious ecthyma, and rapid diagnosis is therefore critical for effective control of the disease. Recently, the invention of a novel LAMP technique that can rapidly amplify nucleic acids with high specificity and sensitivity under isothermal condi...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1526-1
更新日期：2013-04-01 00:00:00
abstract：:Amino acids Lys34, His36, and Phe37 were substituted by PCR-mediated, site-directed mutagenesis for three Trp's in the AcNPV polyhedrin sequence. Phase contrast microscopy revealed refringent, amorphous polyhedra in the nuclei of infected cells. Electron microscopy confirmed a great variation in form and size of the m...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050675
更新日期：2000-01-01 00:00:00
abstract：:Nanoviruses are multi-component plant-infecting single-stranded DNA viruses. Using a viral metagenomics-informed approach, a new nanovirus and two associated alphasatellite molecules have been identified in an uncultivated asymptomatic Vicia cracca plant in the Rhône region of France. This novel nanovirus genome inclu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3634-4
更新日期：2018-03-01 00:00:00
abstract：:Molecular variability was assessed for 18 isolates of cowpea mild mottle virus (CPMMV, genus Carlavirus, family Betaflexiviridae) found infecting soybean in various Brazilian states (Bahia, Goiás, Maranhão, Mato Grosso, Minas Gerais, Pará) in 2001 and 2010. A variety of symptoms was expressed in soybean cv. CD206, ran...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1879-0
更新日期：2014-04-01 00:00:00
abstract：:The E1 nucleic acid sequence of rubella virus strain Judith (RJ) has been cloned into an E. coli expression vector LB03. The reactivity of the expressed unglycosylated antigen (E1J) was compared with its glycosylated counterpart in native virus (RJ) using rabbit and human sera. Rabbit antisera raised against RJ and E1...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317201
更新日期：1992-01-01 00:00:00
abstract：:Human enterovirus 71 (EV71) causes hand, foot and mouth disease in children under 6 years of age, and the neurological complications of this virus can lead to death. Until now, no vaccines or drugs have been available for the clinical control of this epidemic. Macrophages can engulf pathogens and mediate a series of h...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1495-4
更新日期：2013-02-01 00:00:00
abstract：:We observed expression of envelope gp46 protein on an HTLV-II-producing T-cell line (Mo) cells by an immunoelectron microscopical method using a monoclonal antibody against HTLV-II gp46. gp46 reactivity was observed on virus-like particles, extracellular vesicles, cell membrane, and partially in nuclear membrane and e...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322755
更新日期：1995-01-01 00:00:00
abstract：:Papillomavirus capsid proteins L1 and L2 mediate virion attachment, internalization and trafficking. In our studies of the capsid proteins, we identified an interaction of L2 with the E3 ligase Smad ubiquitin regulatory factor 2 (Smurf2). Smurf2 expression alters BPV1 virion trafficking and L2 protein levels. Using BP...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-0924-0
更新日期：2011-05-01 00:00:00
abstract：:Since late 2018, foot-and-mouth disease (FMD) has reemerged and rapidly swept through pig farms in North and Central Vietnam, despite widespread use of commercial FMD vaccines. To investigate the FMD virus (FMDV) strains responsible for the current epidemics, 40 FMDV samples were collected from 17 provinces during Nov...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04763-8
更新日期：2020-11-01 00:00:00
abstract：:Eleven groups of 8 pigs were vaccinated with different vaccinal strains of pseudorabies virus deleted or not for non-essential glycoproteins (gI, gX, gp 63), then were challenged 8-9 weeks later with a virulent strain. Antibodies against the major viral glycoproteins (gII, gIII, gp 50) were titrated at the day of chal...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317144
更新日期：1992-01-01 00:00:00
abstract：:Loop-mediated isothermal amplification (LAMP) of DNA is a novel technique for the amplification of DNA under isothermal conditions. For the first time, we applied this method to develop a simple and sensitive tool for the detection of Pepino mosaic virus (PepMV). PepMV is an emerging pathogen that causes yield and qua...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1706-7
更新日期：2013-10-01 00:00:00
abstract：:In our attempt to isolate mutant cell lines resistant to Newcastle disease virus (NDV) we developed an improved procedure for enrichment of NDV-resistant cells from mouse FM3A cells and isolated a novel NDV-resistant mutant cell line, Had-2, with characteristics different from Had-1, a previously reported NDV-receptor...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310796
更新日期：1994-01-01 00:00:00
abstract：:Murine flank scarification with HSV-1 and -2 results in primary lesions at the site of inoculation within three days and lesions at secondary sites within four days. The severity of the infection can be given a numerical value or "score" which is derived from the number and size of these lesions. Using this model, we ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050030
更新日期：2000-01-01 00:00:00
abstract：:Classic neutralization studies by Fazekas de St. Groth and Webster (8) on mixtures of influenza viruses and mixtures of rabbit antisera are reinterpreted in terms of a percentage contaminant in the stock used for the dilution series. A very small amount of a different virus changes the shape of quantal assay curves co...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317497
更新日期：1979-01-01 00:00:00
abstract：:Wild plants of the family Convolvulaceae are hosts for a few New World begomoviruses (genus Begomovirus, family Geminiviridae). In this work, we report the complete genome sequence of a new begomovirus infecting the wild convolvulaceous plant Jacquemontia tamnifolia in Venezuela. The cloned bipartite genome showed the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3372-7
更新日期：2017-08-01 00:00:00
abstract：:Semliki Forst Virus (SFV) infected chick embroyo cells (CEC) incubated in the presence of Tunicamyein, a drug which specifically inhibits glycosylation, were studied under the electron microscope. No extracellular virions were produced although intracellular viral nucleocapsids in paracrystalline form were detected. U...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314489
更新日期：1977-01-01 00:00:00
abstract：:The amount of viroid RNA required for sequence analysis can be reduced by five to six orders of magnitude when a modified "polymerase chain reaction" (PCR) is used for the amplification of the reversely transcribed, overlapping viroid cDNAs. By applying this procedure it is possible to establish the molecular structur...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313962
更新日期：1989-01-01 00:00:00
abstract：:The Chinese hog cholera lapinized virus (HCLV), also called the "Chinese strain" or "C-strain" of classical swine fever virus (CSFV), was developed in China in the 1950s. There are uncertainties about the genetic heterogeneity and origin of this vaccine virus. The objectives of this study were to investigate the genet...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-0948-5
更新日期：2011-06-01 00:00:00
abstract：:Infectious laryngotracheitis (ILT), caused by infectious laryngotracheitis virus (ILTV), is an Office International des Epizooties (OIE) notifiable disease. However, we have not clearly understood the dynamic distribution, tissue tropism, pathogenesis, and replication of ILTV in chickens. In this report, we investigat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1414-8
更新日期：2013-03-01 00:00:00
abstract：:In 2010, yam beans in a field trial in Peru showed viral disease symptoms. Graft-transmission and positive ELISA results using potyvirus-specific antibodies suggested that the symptoms could be the result of a potyviral infection. Small interfering RNA (siRNA) were extracted from one of the samples and sent for high-t...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1214-6
更新日期：2012-04-01 00:00:00
abstract：:The complete genome sequences of two grapevine virus L (GVL) isolates collected from the wine grape cultivar Blanc du Bois (Vitis spp.: 'Florida D 6-148'×'Cardinal') in Texas were determined. The two genome sequences (excluding the polyA tail) were each 7594 nucleotide long and 99.7% identical to each other, but they ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04682-8
更新日期：2020-08-01 00:00:00
abstract：:Virus isolate Y3V, obtained from tobacco showing leaf curl symptoms in Yunnan, China, had particles with the size and morphology typical of geminiviruses. In reactions with a set of monoclonal antibodies raised against begomoviruses, Y3V was readily differentiated from two previously studied Chinese Begomovirus isolat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170081
更新日期：2001-08-01 00:00:00
abstract：:Porcine epidemic diarrhea virus (PEDV) causes significant loss to the swine industry. The emergence of novel PEDV strains in recent years has decreased the effectiveness of PEDV vaccines. We have developed a live recombinant vaccine, a swinepox virus vector that expresses a truncated S protein (rSPV-St) from a recent ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3548-1
更新日期：2017-12-01 00:00:00
abstract：:During the peak outbreak (July-September 2009), a total 1886 patients were screened in eastern India, of which 139 (7.37%) and 52 (2.76%) were positive for pH1N1 and seasonal H1N1, respectively. Full-length HA1, NA, NS1 and PB1-F2 genes of representative strains were sequenced. Phylogenetic analysis of deduced amino a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0842-6
更新日期：2011-02-01 00:00:00
abstract：:Synthetic peptides comprising the predicted principal neutralizing determinant (PND) in new African and North American HIV-1 clones were tested in ELISA for reactivity with ninety six serum samples from asymptomatic donors in six selected localities in Uganda. Irrespective of the geographical origin of the samples, th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322666
更新日期：1995-01-01 00:00:00
abstract：:An immune adherence hemagglutination test was established for detection of antibodies to respiratory syncytial virus. The test was shown to be as specific as and much more sensitive than the neutralization and complement fixation tests. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309371
更新日期：1984-01-01 00:00:00
abstract：:Purified DNA from 45 isolates of suis herpes virus 1 (SHV1) collected between 1980 and 1987 from clinical outbreaks of Aujeszky's disease on French farms was compared by restriction fragment pattern (RFP) analysis. The BamHI generated RFPs were found to be distinguishable, confirming RFP analysis as a potential epidem...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01323161
更新日期：1990-01-01 00:00:00
abstract：:Genomic DNA of blueberry red ringspot virus (genus Soymovirus, family Caulimoviridae) from highbush blueberry plants growing for years in the Czech Republic and Slovenia was sequenced. The circular dsDNA genomes consist of 8,303 and 8,299 nt, respectively, and contain eight open reading frames (ORFs) longer than 100 a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1077-x
更新日期：2011-10-01 00:00:00
