Sequence analysis of minute amounts of viroid RNA using the polymerase chain reaction (PCR). Rapid communication.

Abstract:

:The amount of viroid RNA required for sequence analysis can be reduced by five to six orders of magnitude when a modified "polymerase chain reaction" (PCR) is used for the amplification of the reversely transcribed, overlapping viroid cDNAs. By applying this procedure it is possible to establish the molecular structure also of those viroids which are present only in extremely low amounts in various crop plants and ornamentals.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Puchta H,Sänger HL

doi

10.1007/BF01313962

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

335-40

issue

3-4

eissn

0304-8608

issn

1432-8798

journal_volume

106

pub_type

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