Establishment of an agroinoculation system for broad bean wilt virus 2.

Abstract:

:We determined the complete nucleotide sequence of a broad bean wilt virus 2 (BBWV-2) isolate from gentian in Japan. The full-length RNA1 and RNA2 sequences, excluding poly(A) tails, were 5955 and 3600 nucleotides long, respectively. Analysis indicated that, in contrast to other BBWV-2 isolates, the 5' end of both RNA1 and RNA2 starts with a GUU sequence. We successfully inoculated Nicotiana benthamiana with BBWV-2 by infiltrating a mixed suspension of two Agrobacterium tumefaciens clones carrying binary vectors with the full-length RNA1 and RNA2 sequences. This is the first report on the efficient, easy and high-throughput use of agroinoculation for generating BBWV-2 infections.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Atsumi G,Tomita R,Kobayashi K,Sekine KT

doi

10.1007/s00705-013-1625-7

subject

Has Abstract

pub_date

2013-07-01 00:00:00

pages

1549-54

issue

7

eissn

0304-8608

issn

1432-8798

journal_volume

158

pub_type

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