Abstract：
:At present, there are no effective antiviral treatments available for contagious ecthyma, and rapid diagnosis is therefore critical for effective control of the disease. Recently, the invention of a novel LAMP technique that can rapidly amplify nucleic acids with high specificity and sensitivity under isothermal conditions has overcome some of the deficiencies of nucleic acid-based diagnostic tests and has made on-site diagnosis possible. To establish a flexible loop-mediated isothermal amplification (LAMP) assay for the rapid detection of orf virus, two pairs of primers, including outer primers F3/B3 and inner primers FIP/BIP, were designed to amplify the DNA polymerase gene. Optimal time and temperature conditions for LAMP were found to be 45 min and 62 °C, respectively. The LAMP assay was shown to be specific, with no cross-reactivity with sheeppox virus, goatpox virus, avian molluscum roup virus or vesicular stomatitis virus. Additionally, the sensitivity of the LAMP method was similar to that of real-time PCR and demonstrated greater sensitivity than a conventional polymerase chain reaction (PCR) assay. To assess the utility of LAMP in the detection of orf virus in clinical samples, a total of 35 samples collected from orf virus-infected sheep and goats were tested using the optimized LAMP assay, real-time PCR, and conventional PCR. Of the samples, 26 were found to be positive by LAMP, and 25 (74.3 %) were positive by real-time PCR, whereas only 18 (51.4 %) were positive by conventional PCR. Our results have shown that the LAMP assay developed in this study can be used for the rapid detection of orf virus.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Li J,Song D,He W,Bao Y,Lu R,Su G,Wang G,Lu H,Zhao K,Gao Fdoi
10.1007/s00705-012-1526-1subject
Has Abstractpub_date
2013-04-01 00:00:00pages
793-8issue
4eissn
0304-8608issn
1432-8798journal_volume
158pub_type
杂志文章abstract：:A viroid was isolated from symptomless Coleus blumei cultivar (cv.) 'Rainbow Gold' plants using the bidirectional PAGE method for analysis of small circular RNA molecules. The viriod was transmitted to viroid-free plants of Coleus blumei cv. 'Scarlet Dragonfly' by mechanical inoculation. Cloning and sequencing reveale...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718222
更新日期：1996-01-01 00:00:00
abstract：:Different patterns of disease were observed among 11 chimpanzees who were inoculated intravenously with hepatitis E virus (HEV) positive fecal specimens from four different outbreaks (Nepal 1981, Uzbekistan 1981, Pakistan 1985, and Mexico 1986). Five chimpanzees had marginal or no liver enzyme elevations within 70 day...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070065
更新日期：2000-01-01 00:00:00
abstract：:Atazanavir (ATZ) is a well-tolerated protease inhibitor that can be boosted with ritonavir (r) to treat infection with resistant strains of human immunodeficiency virus 1 (HIV-1). The aim of this meta-analysis was to compare the efficacy, safety, and metabolic effects of ATZ/r regimen versus commonly used antiretrovir...
journal_title：Archives of virology
pub_type： 杂志文章,meta分析,评审
doi：10.1007/s00705-017-3346-9
更新日期：2017-08-01 00:00:00
abstract：:A small unconventional, viroid-like aetiological agent might initiate Crohn's disease (CD). Electrophoresis of radiolabelled low molecular weight nucleic acids from mesenteric lymph nodes and leucocytes did not distinguish CD-specific sequences compatible with a viroid-like agent. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310895
更新日期：1986-01-01 00:00:00
abstract：:In attempts to further develop murine leukemia virus (MLV) based retroviral vectors for gene therapy, we investigated vector production and antisense expression from retroviral constructs with U3 deletions or insertions. Promoter elements in the U3 region of the 3' LTR of the vector pLXSN were deleted and replaced wit...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170029
更新日期：2001-01-01 00:00:00
abstract：:Intravenous polyoma virus inoculation into pregnant Wistar rats resulted in transplacental infection of the foetus, causing tumours and hydronephroses. Cyclosporin A reduced these effects significantly. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313783
更新日期：1993-01-01 00:00:00
abstract：:The oncogene E6 of human papillomavirus 8, which is associated with skin cancers in epidermodysplasia verruciformis, was transcribed and translated in vitro. The resulting 17 kDa protein did not bind to the cellular p53 in contrast to E6 of HPV16. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309654
更新日期：1992-01-01 00:00:00
abstract：:Peach asteroid spot (PAS) and peach sooty ringspot (PSRS) are two diseases of stone fruit trees of unknown aetiology. The use of a cRNA probe of the newly described Apricot latent virus (ApLV), a tentative member of the Foveavirus genus, indicated the presence of cross-hybridizing agents in PAS isolate LA2 and in PSRS...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170071
更新日期：2001-08-01 00:00:00
abstract：:Noroviruses belong to a genus of genetically diverse viruses within the family Caliciviridae and cause acute gastroenteritis in humans and animals. They are subdivided into genogroups, each of which further segregates into genotypes. Until recently, a new genotype was based on a defined pairwise distance cutoff of com...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1708-5
更新日期：2013-10-01 00:00:00
abstract：:The E1 nucleic acid sequence of rubella virus strain Judith (RJ) has been cloned into an E. coli expression vector LB03. The reactivity of the expressed unglycosylated antigen (E1J) was compared with its glycosylated counterpart in native virus (RJ) using rabbit and human sera. Rabbit antisera raised against RJ and E1...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317201
更新日期：1992-01-01 00:00:00
abstract：:Few reports are available on HCV molecular epidemiology among IDUs in Eastern Europe, and none in Montenegro. The aim of this study was to investigate the HCV genotype distribution in Montenegro among IDUs and to perform Bayesian and evolutionary analysis of the most prevalent HCV genotype circulating in this populati...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3224-5
更新日期：2017-06-01 00:00:00
abstract：:Rabies is one of the most important zoonotic diseases and is caused by several rabies virus (RABV) variants. These variants can exhibit differences in neurovirulence, and few studies have attempted to evaluate the neuroinvasiveness of variants derived from vampire bats and wild carnivores. The aim of this study was to...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3530-y
更新日期：2017-12-01 00:00:00
abstract：:A competitive-type solid phase radioimmunoassay (RIA) was developed for the detection of antibody to bovine polyomavirus. Comparison of RIA and counter-immunoelectrophoresis (CIE) results on 273 cattle sera indicated that both techniques were detecting antibody of like specificity. Human sera from 256 blood donors, 21...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315306
更新日期：1986-01-01 00:00:00
abstract：:A method for purification of Hog Cholera Virus (HCV) is presented. Cell-associated virus was extracted from PK15 cells 17 hours p.i. by fluorocarbon treatment. The virus was concentrated by polyethylene glycol precipitation and partially purified by pelleting on a fluorocarbon cushion. Final purification was achieved ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314377
更新日期：1977-01-01 00:00:00
abstract：:Immunofluorescence assay using monospecific and monoclonal antibodies to the 65 K major protein of human cytomegalovirus (HCMV) was carried out to monitor the expression of this protein in infected cells. Regardless of differences in the reactivity of the monoclonal antibodies, as determined by immunoblotting and immu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314653
更新日期：1988-01-01 00:00:00
abstract：:Molecular studies have been carried out on Tomato mild mottle virus (TMMV) and 2322 nucleotides of the aphid-transmitted virus have been sequenced. This translates to a 2187 nucleotide open reading frame and a 135 nucleotide untranslated region (UTR) followed by a poly A tail. The sequence is consistent with the NIb a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170013
更新日期：2001-12-01 00:00:00
abstract：:Between 2005 and 2011, 23 lineages of circulating vaccine-derived polioviruses (cVDPVs) were detected in Nigeria with nonstructural region (NSR) of non-polio enterovirus C (NPEV-C) origin. However, no information exists on NPEV-C strains recombining with oral poliovirus type 2 vaccine strains (OPV2) to make type 2 cVD...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2322-x
更新日期：2015-03-01 00:00:00
abstract：:Persistent infections with high-risk human papillomaviruses (HR-HPVs) are linked to the development of cervical cancer due to a deregulation of the productive viral cycle in the host cell, leading to cell transformation. The E2 viral protein is expressed early during an HPV infection and regulates viral replication an...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-04124-6
更新日期：2019-07-01 00:00:00
abstract：:In this cross-sectional study, we evaluated the efficiency of the plasma of 38 antiretroviral-naïve HIV-1-infected children from northern India against a standard panel of pseudoviruses (3 clade C and 3 clade B) by TZM-bl assay. Neutralization potential was observed to a variable extent, with a potency ranging up to r...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1357-0
更新日期：2012-09-01 00:00:00
abstract：:Four epidemic seasons of rotaviruses were studied in Helsinki during 1986-1990. This is the first Scandinavian study, where both electropherotypes and serotypes are determined. Out of 5316 fecal specimens 769 (14.5%) rotavirus positive samples were detected by electron microscopy. Of these, 645 isolates (83.9%) gave a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314964
更新日期：1995-01-01 00:00:00
abstract：:Macrophage (mac)-tropic human immnunodeficiency virus type 1 (HIV-1) and simian immnunodeficiency virus (SIV) in brain are associated with neurological disease. Mac-tropic HIV-1 evolves enhanced CD4 interactions that enable macrophage infection via CD4, which is in low abundance. In contrast, mac-tropic SIV is associa...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-4094-1
更新日期：2019-02-01 00:00:00
abstract：:African swine fever virus (ASFV) was detected in wild boar in eastern Poland in early 2014. So far, 65 cases of ASFV infection in wild boar have been recognised. The methods used for ASFV detection included highly specific real-time PCR with a universal probe library (UPL), enzyme-linked immunosorbent assay (ELISA), a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2650-5
更新日期：2016-01-01 00:00:00
abstract：:Loop-mediated isothermal amplification (LAMP) of DNA is a novel technique for the amplification of DNA under isothermal conditions. For the first time, we applied this method to develop a simple and sensitive tool for the detection of Pepino mosaic virus (PepMV). PepMV is an emerging pathogen that causes yield and qua...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1706-7
更新日期：2013-10-01 00:00:00
abstract：:To determine the prevalence of enteroviruses in aquatic environments in Shenzhen, water samples were collected monthly from April 2010 to December 2011. After concentration by the filter adsorption-beef extract elution method, the enteroviruses were propagated in RD cells. Thirty-five of 105 water samples (33.33 %) we...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1611-0
更新日期：2013-06-01 00:00:00
abstract：:A novel potyvirus was discovered in Angelica lucida L. (wild celery) and A. genuflexa Nutt. (kneeling Angelica) (family Apiaceae) in the Matanuska- Susitna Valley, Alaska. The experimental plant host range of the virus included species in three families: Chenopodiaceae (Chenopodium amaranticolor Coste et Reyn and Chen...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-1003-4
更新日期：2007-01-01 00:00:00
abstract：:Adult Balb/c mice were passively immunized with monoclonal antibodies (100 micrograms/mouse) raised against tick-borne encephalitis (TBE) virus then challenged 24 hours later s.c. with 10 LD50 of TBE virus (Nëudorfl isolate). None of the mice showed evidence of premature death although all except one of the monoclonal...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310983
更新日期：1987-01-01 00:00:00
abstract：:In order to differentiate recent isolates of avian infectious bronchitis virus (IBV) in Taiwan, polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and direct sequencing methods were used to type 25 IBV Taiwan isolates. Two conserved sequences that flank the hypervariable region I (HVR I)...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718291
更新日期：1996-01-01 00:00:00
abstract：:Seven putative mitoviral genomes, representing four species from three Sclerotinia sclerotiorum isolates, were fully sequenced. The genome lengths ranged from 2438 to 2815 nucleotides. The RNA-dependent RNA polymerase (RdRp) of one genome shared high amino acid (aa) sequence identity (98.5 %) with the previously descr...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2171-7
更新日期：2014-11-01 00:00:00
abstract：:Utilizing co-transfection of DNA from glycoprotein gB- strain of HSV1 and cloned fragments of several simian alpha-herpesviruses containing the UL26, UL27 (gB glycoprotein), and UL28 gene homologs, replication-competent recombinant viruses were produced. Genetic analysis of one HSV1/SA8 recombinant (HSV1/SgB) demonstr...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050114
更新日期：1997-01-01 00:00:00
abstract：:The in vitro cytopathology and the neurovirulence to inbred mice demonstrated by five variants originally derived from one laboratory strain (Miyama) of type 1 herpes simplex virus (HSV-1) were studied comparatively. Three of the variants are syncytial [+GC (LPV), +GC (SPV), +GC (81)] and two are non-syncytial [-GCr a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317369
更新日期：1986-01-01 00:00:00
