Detection of Pepino mosaic virus isolates from tomato by one-step reverse transcription loop-mediated isothermal amplification.

Abstract:

:Loop-mediated isothermal amplification (LAMP) of DNA is a novel technique for the amplification of DNA under isothermal conditions. For the first time, we applied this method to develop a simple and sensitive tool for the detection of Pepino mosaic virus (PepMV). PepMV is an emerging pathogen that causes yield and quality losses in tomato crops. Specific RT-LAMP primers for PepMV detection were designed based on triple gene block sequences. The reaction was performed in a single tube at 65 °C for 30 min. The RT-LAMP assay is easy to perform and inexpensive, and it may be applied in the rapid and specific diagnosis of PepMV.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Hasiów-Jaroszewska B,Borodynko N

doi

10.1007/s00705-013-1706-7

subject

Has Abstract

pub_date

2013-10-01 00:00:00

pages

2153-6

issue

10

eissn

0304-8608

issn

1432-8798

journal_volume

158

pub_type

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