Abstract：
:The structure of the scrapie agent remains unknown. However, scrapie infectivity tends to co-sediment with an infection specific fraction of the glycoprotein PrP (PrPSc) under conditions which solubilise the normal form of this protein (PrPc); accordingly, PrP has been proposed as a candidate component of the agent. To investigate this further we have been examining a new scrapie-related murine model in conjunction with established scrapie models. A bovine spongiform encephalopathy (BSE) derived murine model has short incubation periods, high infectivity titre and low amounts of PrP deposited in the brain. A membrane fraction from scrapie/BSE infected brain is solubilised with Sarkosyl at pH > or = 9.0. Most PrP is also solubilised. In models of the disease with little deposition of the PrP in the brain, this solubilisation step is particularly effective in reducing the amounts of PrP sedimented from brain extracts. Gradient centrifugation of the sedimented fraction shows further separation of infectivity and the residual PrP. It is concluded that at least some PrPSc in the brain need not be associated directly with infectious agents but is deposited in brain solely as a pathological product of infection. However, a residual sedimentable fraction contains PrP which may be a component of the agent.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Somerville RA,Dunn AJdoi
10.1007/BF01718399subject
Has Abstractpub_date
1996-01-01 00:00:00pages
275-89issue
2eissn
0304-8608issn
1432-8798journal_volume
141pub_type
杂志文章abstract：:Porcine circovirus type 2 (PCV2) is a member of the genus Circovirus in the family Circoviridae. Most subgenomic molecules of PCV2 have been mapped. Here, the first full-length sequence of a subviral molecule of PCV2 (CH-IVT12) containing a reverse complement sequence of the PCV2 genome was determined by sequencing DN...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2859-y
更新日期：2016-07-01 00:00:00
abstract：:The ability of human fibroblast interferon to suppress colony formation and papovavirus T-antigen expression in six human tumor cell lines did not appear to correlate with their content of chromosomes 16 and 21. Additional factors or chromosomes may be involved in the manifestation of interferon action in aneuploid ce...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317505
更新日期：1979-01-01 00:00:00
abstract：:Ten strains of avian infectious bronchitis virus (IBV) were titrated as plaque-forming units in primary chick embryo fibroblast cells. In the absence of trypsin, plaques were only formed by Beaudette-42 and Iowa-609 strains. When trypsin was incorporated in the overlay medium of cell monolayers, all the IBV strains te...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320246
更新日期：1981-01-01 00:00:00
abstract：:Bacteriophage JBP901, isolated from fermented food, is specific for Bacillus cereus group species and exhibits a broad host spectrum among a large number of B. cereus isolates. Genome sequence analysis revealed a linear 159,492-bp genome with overall G+C content of 39.7 mol%, and 201 ORFs. The presence of a putative m...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2485-0
更新日期：2015-09-01 00:00:00
abstract：:Work from a number of laboratories including our own has shown that foreign B-epitopes inserted into the c/e1-region of Hepatitis B core antigen (HBcAg) elicit powerful antibody responses when mice are immunised with the recombinant core particles. In the present study, we wished to take advantage of the immunodominan...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050590
更新日期：1999-01-01 00:00:00
abstract：:This report describes the first isolation and partial characterization of a herpesvirus from the harbor seal (Phoca vitulina). The virus was isolated during a disease outbreak in a group of young seals nursed in a seal orphanage in The Netherlands. Almost half of the seals died with symptoms of acute pneumonia and foc...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309828
更新日期：1985-01-01 00:00:00
abstract：:The amount of viroid RNA required for sequence analysis can be reduced by five to six orders of magnitude when a modified "polymerase chain reaction" (PCR) is used for the amplification of the reversely transcribed, overlapping viroid cDNAs. By applying this procedure it is possible to establish the molecular structur...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313962
更新日期：1989-01-01 00:00:00
abstract：:The polypeptides associated with fowlpox virus (FPV) infection of chicken embryo skin (CES) cells were examined by metabolic labelling with [35S]-methionine and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Polypeptide synthesis was followed over the first 48 hours post infection, as this was s...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320959
更新日期：1987-01-01 00:00:00
abstract：:Despite reports that the PB1-F2 protein contributes to influenza virus pathogenicity in the mouse model, little is known about its significance in avian hosts. In our previous study, the A/Vietnam/1203/04 (H5N1) wild-type virus (wtVN1203) was more lethal to mallard ducks than a reverse genetics (rg)-derived VN1203. In...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0666-4
更新日期：2010-06-01 00:00:00
abstract：:A method for purification of Hog Cholera Virus (HCV) is presented. Cell-associated virus was extracted from PK15 cells 17 hours p.i. by fluorocarbon treatment. The virus was concentrated by polyethylene glycol precipitation and partially purified by pelleting on a fluorocarbon cushion. Final purification was achieved ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314377
更新日期：1977-01-01 00:00:00
abstract：:The CPK cells derived from swine kidney were infected with the attenuated TO-163 strain of transmissible gastroenteritis (TGE) virus, and fused with uninfected Vero cells in the presence of polyethylene glycol. Repeated cocultivation of the fused cells with uninfected Vero cells rendered the virus to grow in Vero cell...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01321128
更新日期：1992-01-01 00:00:00
abstract：:Cotton leaf curl disease (CLCuD) is a major limitation to cotton production on the Indian subcontinent. A survey for viruses causing CLCuD was conducted during the 2009 and 2010 cropping seasons in the northwestern Indian cotton-growing belt in the states of Punjab, Haryana and Rajasthan. Partial sequences of 258 and ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1225-y
更新日期：2012-05-01 00:00:00
abstract：:An archival brain sample from an infected dog taken during the 1988/89 rabies outbreak in Finland was investigated to confirm that it was infected with classical rabies virus (genotype 1). The first 400 base pairs of the virus nucleoprotein gene were then sequenced and used to undertake a phylogenetic comparison betwe...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0523-z
更新日期：2005-07-01 00:00:00
abstract：:A putative chrysovirus recovered from Brassica campestris var. purpurea and provisionally named "Brassica campestris chrysovirus 1" (BrcCV1) was sequenced. The genome of the putative BrcCV1 consists of three double-stranded RNAs (dsRNAs) comprising 3,639 (dsRNA 1), 3,567 (dsRNA 2) and 3,337 (dsRNA 3) base pairs, respe...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3196-x
更新日期：2017-04-01 00:00:00
abstract：:H9N2 avian influenza virus has caused huge economic loss for the Chinese poultry industry since it was first identified. Vaccination is frequently used as a control method for the disease. Meanwhile suspension culture has become an important tool for the development of influenza vaccines. To optimize the suspension cu...
journal_title：Archives of virology
pub_type： 临床试验,杂志文章
doi：10.1007/s00705-017-3460-8
更新日期：2017-10-01 00:00:00
abstract：:Rabies is one of the most important zoonotic diseases and is caused by several rabies virus (RABV) variants. These variants can exhibit differences in neurovirulence, and few studies have attempted to evaluate the neuroinvasiveness of variants derived from vampire bats and wild carnivores. The aim of this study was to...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3530-y
更新日期：2017-12-01 00:00:00
abstract：:Simian varicella virus (SVV) infection of non-human primates is used as a model to study the pathogenesis and latency of varicella-zoster virus (VZV), the etiological agent of chickenpox and shingles. Uracil DNA glycosylase (UDG) is a DNA repair enzyme responsible for excision of uracil residues misincorporated into D...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050630
更新日期：1999-01-01 00:00:00
abstract：:Mouse embryo fibroblasts and mouse adrenal tumor cells support the replication of encephalomyocarditis (EMC) virus, whereas rat glial and rat hepatoma cells are non-permissive. These differences in susceptibility were not due to the lack of virus attachment to rat cells. The findings that rat cells could be transfecte...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310884
更新日期：1986-01-01 00:00:00
abstract：:We determined the complete genome sequence of a South Korean (SK) isolate of Brugmansia mosaic virus (BruMV), which has recently been proposed to be a member of a new species in the genus Potyvirus. The genomic RNA of BruMV isolate SK is 9781 nucleotides in length (excluding the 3'-terminal poly (A)) and shares comple...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1693-8
更新日期：2013-09-01 00:00:00
abstract：:In this study, RNA corresponding to bovine enterovirus (BEV) was detected in 24.6 % of faecal samples (17/69) from diarrheic and healthy cattle in six different areas in China by an RT-PCR screening method. Furthermore, two cytopathic agents, designated as BHM26 and BJ50, were isolated from the bovine diarrheic fecal ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1424-6
更新日期：2012-12-01 00:00:00
abstract：:RNase resistance analyses of native, heat-denatured and self-annealed L and S species of Machupo virion RNA revealed the presence of complementary sequences. Self-annealing was concentration-dependent. The data indicate, that complementary sequences were present in separate RNA strands. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310900
更新日期：1986-01-01 00:00:00
abstract：:During the course of hominoid evolution, a new transcript variant of the GSDML (gasdermin-like protein) gene was formed by the integration of the antisense-oriented HERV-H (human endogenous retrovirus) LTR (long terminal repeat) element. To investigate regions that are critical for transcriptional regulation of the GS...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0105-y
更新日期：2008-01-01 00:00:00
abstract：:The M protein, encoded by the porcine reproductive and respiratory syndrome virus (PRRSV) ORF6 gene, is considered to be one of the most conserved PRRSV proteins. In recent decades, highly specific monoclonal antibodies (Mabs) have been exploited to provide reliable diagnoses for many diseases. In this study, two diff...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2174-4
更新日期：2014-11-01 00:00:00
abstract：:Human polymorphonuclear leukocytes (PMNL) incubated with influenza virus, A/USSR/90/77 (H1N1) or its hemagglutinin produced a significant increase in their PKC activity when compared with untreated PMNL. The activated kinase translocated from cytosol to cellular membrane. The calcium-dependent enzyme activity was inhi...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050439
更新日期：1998-01-01 00:00:00
abstract：:Ljungan virus is a recently identified member of the family Picornaviridae that was isolated from bank voles in Sweden. LjV has been associated with [corrected] type 1 diabetes-like symptoms and myocarditis in bank voles (Myodes glareolus), and it has been suggested that it has zoonotic potential. Here, we show for th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1731-6
更新日期：2014-03-01 00:00:00
abstract：:The complete genome sequences of two grapevine virus L (GVL) isolates collected from the wine grape cultivar Blanc du Bois (Vitis spp.: 'Florida D 6-148'×'Cardinal') in Texas were determined. The two genome sequences (excluding the polyA tail) were each 7594 nucleotide long and 99.7% identical to each other, but they ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04682-8
更新日期：2020-08-01 00:00:00
abstract：:This study aimed at investigating the effect of progesterone on interferon signaling pathways in peripheral blood mononuclear cells (PBMCs) of patients infected with hepatitis C virus (HCV). PBMCs were isolated from peripheral blood of 38 treatment-naïve HCV-infected patients, pooled, and stimulated with progesterone ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1673-z
更新日期：2013-08-01 00:00:00
abstract：:The present study on genetic diversity of human papillomaviruses in women infected by HIV in Brazil describes the frequency, the genotypes, and five new variants of HPV. One hundred fifty cervical smears of HIV-positive women were subjected to cytological examination, and the DNA samples obtained were assayed by MY09/...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0828-6
更新日期：2007-01-01 00:00:00
abstract：:A plaque assay was developed for feline parvovirus (FPV; feline panleucopaenia virus) in a feline embryo (FEmb) cell line. Higher numbers and larger diameter plaques were obtained with a) seeding rates of 0.7 X 10(5) and 1.5 X 10(5) cells cf. 3 X 10(5) and 6 X 10(5) cells/well of 35 mm diameter, b) synchronised cells ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01378978
更新日期：1985-01-01 00:00:00
abstract：:We detected a novel bovine hepacivirus N (HNV) subtype, IME_BovHep_01, in the serum of cattle in Tengchong, Yunnan, China, by high-throughput sequencing. The complete genome of IME_BovHep_01, was sequenced using an Illumina MiSeq sequencer and found to be 8850 nt in length, encoding one hypothetical protein. BLASTn an...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04611-9
更新日期：2020-06-01 00:00:00
