Plaque formation by avian infectious bronchitis virus in primary chick embryo fibroblast cells in the presence of trypsin.

Abstract:

:Ten strains of avian infectious bronchitis virus (IBV) were titrated as plaque-forming units in primary chick embryo fibroblast cells. In the absence of trypsin, plaques were only formed by Beaudette-42 and Iowa-609 strains. When trypsin was incorporated in the overlay medium of cell monolayers, all the IBV strains tested produced plaques within 4 days after inoculation. Incorporation of 20-40 microgram of trypsin per ml of the overlay medium seemed to be suitable for plaque formation of IBV. A preliminary investigation was made of the mode of action of trypsin.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Otsuki K,Tsubokura M

doi

10.1007/BF01320246

subject

Has Abstract

pub_date

1981-01-01 00:00:00

pages

315-20

issue

4

eissn

0304-8608

issn

1432-8798

journal_volume

70

pub_type

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