Rapid characterization of new pestivirus strains by direct sequencing of PCR-amplified cDNA from the 5' noncoding region.

Abstract:

:Reverse transcription coupled with the polymerase chain reaction (RT-PCR) was used for the rapid laboratory diagnosis of pestivirus infections. A direct DNA sequencing method was developed for the analysis of the amplified cDNA from the 5' noncoding region of the viral genome. 70 pestivirus strains were compared in this study. Sequence analysis allowed the characterization of each isolate as either classical swine fever virus (CSFV), bovine viral diarrhea virus, or border disease virus, respectively. The 48 CSFV strains could be further classified into several subgroups, which correlated either with the geographical origin or the date of the first isolation of the respective isolate.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Hofmann MA,Brechtbühl K,Stäuber N

doi

10.1007/BF01309467

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

217-29

issue

1-2

eissn

0304-8608

issn

1432-8798

journal_volume

139

pub_type

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