Abstract：
:The Pasteur strain of fixed rabies virus was adapted to primary cell cultures of Japanese quail embryos and designated as MNIIVP-74. In the course of adaptation the virus pathogenicity for rabbits by the intracerebral route decreased considerably and the pathogenicity for rabbits and adult white mice by extraneural routes was completely lost. After inoculation of Japanese quail embryo cell cultures, a titer of the virus in the culture fluid at 4 days was 6.25--7.0 lg LD50/ml (by the intracerebral inoculation of adult white mice). Viral antigen could be detected by immunofluorescence in the cytoplasm of approximately 60 per cent of the cells. Virus multiplication was accompanied by intensive interferon production. In cultures of BHK-21/13S cells the titer of the virus reached was 5.75 lg LD50/ml at 24 hours and about 30 per cent of the cells were affected. The MNIIVP-74 virus showed a high immunogenic activity in rabbits, guinea pigs and mice.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Bektemirova MS,Osidze DF,Pille ER,Nadaichik LV,Matevosyan KS,Nagieva FG,Bogomolova NN,Boriskin YS,Yanova NNdoi
10.1007/BF01320592subject
Has Abstractpub_date
1979-01-01 00:00:00pages
61-8issue
1-2eissn
0304-8608issn
1432-8798journal_volume
61pub_type
杂志文章abstract：:The entry modes of Japanese encephalitis (JE) and dengue-2 (DEN-2) viruses into C6/36 mosquito cells and of DEN-2 virus into human peripheral blood monocytes in vitro were studied. Inoculation of either JE or DEN-2 virions into C6/36 cells resulted in direct penetration of the virions into the cytoplasm at the cell su...
journal_title：Archives of virology
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journal_title：Archives of virology
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doi：10.1007/BF01318009
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journal_title：Archives of virology
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doi：10.1007/BF01320246
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更新日期：2005-02-01 00:00:00
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更新日期：1998-01-01 00:00:00
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更新日期：1978-01-01 00:00:00
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更新日期：1987-01-01 00:00:00
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journal_title：Archives of virology
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doi：10.1007/BF01317266
更新日期：1992-01-01 00:00:00
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doi：10.1007/BF01310767
更新日期：1991-01-01 00:00:00
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更新日期：2015-06-01 00:00:00
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更新日期：1979-01-01 00:00:00
abstract：:A small unconventional, viroid-like aetiological agent might initiate Crohn's disease (CD). Electrophoresis of radiolabelled low molecular weight nucleic acids from mesenteric lymph nodes and leucocytes did not distinguish CD-specific sequences compatible with a viroid-like agent. ...
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更新日期：1986-01-01 00:00:00
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更新日期：2011-09-01 00:00:00
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更新日期：2020-11-01 00:00:00
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doi：10.1007/BF02175596
更新日期：1975-01-01 00:00:00
abstract：:Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. In 2013, a large outbreak was reported on the archipelago of French Polynesia. In this study, we report the detection and molecular characterization of Zika virus for the first time in Chile from an outbreak among the inhabitant...
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更新日期：2016-03-01 00:00:00
