Evidence for in vitro and in vivo autocatalytic processing of the primary translation product of beet necrotic yellow vein virus RNA 1 by a papain-like proteinase.

Abstract:

:Beet necrotic yellow vein virus RNA 1 contains a single long ORF corresponding to the theoretical translation product of 237 kDa which contains the information necessary for replication of the viral genome. This ORF contains a putative papain-like proteinase domain which has been localized, on the basis of sequence alignments, between the helicase and polymerase domains. Here we show that the RNA 1 primary translation product can be cleaved autocatalytically in vitro into two species of 150 kDa and 66 kDa, the latter of which probably contains the entire polymerase domain. A 66 kDa protein was detected immunologically in infected C. quinoa protoplasts using an antiserum specific for the C-terminal region of the RNA 1 primary translation product, confirming that processing also occurs in vivo.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Hehn A,Fritsch C,Richards KE,Guilley H,Jonard G

doi

10.1007/s007050050141

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

1051-8

issue

5

eissn

0304-8608

issn

1432-8798

journal_volume

142

pub_type

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