Abstract:
:Beet necrotic yellow vein virus RNA 1 contains a single long ORF corresponding to the theoretical translation product of 237 kDa which contains the information necessary for replication of the viral genome. This ORF contains a putative papain-like proteinase domain which has been localized, on the basis of sequence alignments, between the helicase and polymerase domains. Here we show that the RNA 1 primary translation product can be cleaved autocatalytically in vitro into two species of 150 kDa and 66 kDa, the latter of which probably contains the entire polymerase domain. A 66 kDa protein was detected immunologically in infected C. quinoa protoplasts using an antiserum specific for the C-terminal region of the RNA 1 primary translation product, confirming that processing also occurs in vivo.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Hehn A,Fritsch C,Richards KE,Guilley H,Jonard Gdoi
10.1007/s007050050141subject
Has Abstractpub_date
1997-01-01 00:00:00pages
1051-8issue
5eissn
0304-8608issn
1432-8798journal_volume
142pub_type
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