Abstract：
:Five cDNA probes prepared from molecular clones representing genomic RNA sequences of the virulent Miller strain of transmissible gastroenteritis virus (TGEV) were used in a dot blot hybridization assay to detect TGEV in cell culture and fecal specimens. Two clones (pA2 and pB4) represent nucleotide base pairs at the 3' terminus of the Miller TGEV genome. The other three clones represent various portions of the 5' end of the E2 gene, which codes for the major surface glycoprotein of TGEV. Each of the 32P-labeled cDNA probes hybridized to the virulent Miller, attenuated Purdue and four field strains of TGEV. The probes detected 200 to 2000 pg of TGEV RNA extracted from density gradient purified virions and did not hybridize RNA from mock-infected cell cultures, porcine rotavirus or antigenically unrelated coronaviruses. The pB4 and Hpa-1600 probes detected TGEV RNA sequences in 79 and 88%, respectively of 34 field samples identified as TGEV positive by the immunofluorescence assay and electron microscopy (EM). The pD24 clone, which is able to differentiate TGEV from the antigenically related coronaviruses, also compared favorably with conventional methods of EM and immunofluoresence for the detection of TGEV in fecal specimens.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Benfield DA,Jackwood DJ,Bac I,Saif LJ,Wesley RDdoi
10.1007/BF01319234subject
Has Abstractpub_date
1991-01-01 00:00:00pages
91-106issue
1-4eissn
0304-8608issn
1432-8798journal_volume
116pub_type
杂志文章abstract：:We investigated the transforming domain of a recently isolated feline sarcoma virus (TP1-FeSV) which encodes a fgr-related tyrosine kinase expressed as a gag-fgr fusion protein. The gag portion was removed and replication-competent expression vectors (RCAS) with inserted v-fgr sequences were established. Chicken embry...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01379113
更新日期：1994-01-01 00:00:00
abstract：:The genomes of a large number of highly diverse novel circular DNA viruses from a wide range of sources have been characterised in recent years, including circular single-stranded DNA (ssDNA) viruses that share similarities with plant-infecting ssDNA viruses of the family Geminiviridae. Here, we describe six novel cir...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2358-6
更新日期：2015-05-01 00:00:00
abstract：:An archival brain sample from an infected dog taken during the 1988/89 rabies outbreak in Finland was investigated to confirm that it was infected with classical rabies virus (genotype 1). The first 400 base pairs of the virus nucleoprotein gene were then sequenced and used to undertake a phylogenetic comparison betwe...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0523-z
更新日期：2005-07-01 00:00:00
abstract：:The effect of temperature on the response of tomato (Lycopersicon esculentum Mill. cv. Rutgers) to infections with the necrogenic cucumber mosaic virus (CMV) satellite D-CARNA 5 was investigated with each of four CMV strains D, 1, Y and S functioning as helper virus. At 24 degrees C lethal necrosis was observed in all...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309724
更新日期：1995-01-01 00:00:00
abstract：:Genetic relationships between the genome segments of six cypovirus (CPV) isolates were analysed by RNA cross-hybridisation. These included three type 1 viruses and single isolates of types 2, 5 and 12, which collectively are identical to those previously compared by serology and electrophoresis [Mertens et al. (1989),...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050525
更新日期：1999-01-01 00:00:00
abstract：:African swine fever (ASF) a lethal, viral hemorrhagic disease of domestic pigs, first reported from East Africa in 1921, is still widespread in this region. In order to assess field heterogeneity at the regional level, nucleotide sequences corresponding to the C-terminal end of the p72 gene were determined for 77 ASF ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0602-1
更新日期：2005-12-01 00:00:00
abstract：:The diagnosis of cytomegalovirus retinitis (CMV-R) is difficult and usually based on clinical criteria or invasive diagnostic procedures. The purpose of this study was to investigate a possible association between CMV-R and specific anti-CMV antibodies in tears. Paired tear and serum samples were obtained from 96 indi...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0813-0
更新日期：2006-12-01 00:00:00
abstract：:A potyvirus isolated from Pinellia ternata in China was characterised and shown to be related to Soybean mosaic virus (SMV). The virus was pathogenic on P. ternata and some soybean cultivars, whereas the local soybean SMV isolate HH5 did not infect P. ternata. Western blot experiments demonstrated a serological relati...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0184-8
更新日期：2004-02-01 00:00:00
abstract：:In cultured chicken embryo fibroblasts treated with copper-(copper cation 10-minus 3.5-10-minus 5 M), nickel and cobalt salts an enhancement of the plating efficiency of Semliki Forest virus could be observed. This augmented plaque formation was most probably due to a higher adsorption rate of virions to the cell surf...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315593
更新日期：1975-01-01 00:00:00
abstract：:Expression of the entire open reading frame of hepatitis A virus was achieved in a vaccinia virus/T7/EMCV hybrid system. The expressed antigens were characterized as particles with buoyant densities and sedimentation coefficients typical of empty capsids and pentamers of HAV. This was further confirmed by electron mic...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310029
更新日期：1994-01-01 00:00:00
abstract：:A papovavirus was isolated from the urine of a 24 year old female who underwent bone marrow transplantation after a relapse of acute myeloid leukemia. The virus (JL) resembles BK virus in its antigenic and growth properties, but has a different restriction endonuclease pattern after digestion with the restriction endo...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315635
更新日期：1978-01-01 00:00:00
abstract：:Seven isolates of West Nile virus and an additional isolate tentatively identified at the outset as West Nile virus, were compared by Casals' modified haemagglutination inhibition technique. Two of the isolates were nearly identical; a second set of five isolates cross-reacted among themselves within a wide range and ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315279
更新日期：1978-01-01 00:00:00
abstract：:The entry modes of Japanese encephalitis (JE) and dengue-2 (DEN-2) viruses into C6/36 mosquito cells and of DEN-2 virus into human peripheral blood monocytes in vitro were studied. Inoculation of either JE or DEN-2 virions into C6/36 cells resulted in direct penetration of the virions into the cytoplasm at the cell su...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313814
更新日期：1989-01-01 00:00:00
abstract：:At least 28 polypeptides have been identified in intracellular virus, with molecular weights ranging from 11,500 to 243,000 daltons. By treatment with Nonidet P-40 and 2-mercaptoethanol it is possible to obtain subviral particles that have lost some proteins and have a density in CsCl of 1.31 g/cm3 which is higher tha...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314979
更新日期：1980-01-01 00:00:00
abstract：:Using a one-step model for cell-to-cell transmission of HIV infection we have identified two distinct phases of HIV RNA synthesis. The first phase (4 h-12 h p.i.) was marked by an increase in only the full-length 9 kb RNA, while the second phase (24 h p.i. onwards) comprised a significant increase in the levels of all...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050429
更新日期：1998-01-01 00:00:00
abstract：:Using random amplification and high-throughput sequencing technology a novel picornavirus with dicistronic genome organization and genetically related to canine picodicistrovirus (genus Dicipivirus, family Picornaviridae) was identified and characterized in Northern white-breasted hedgehogs. Hedgehog dicipivirus (hedg...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3565-0
更新日期：2018-01-01 00:00:00
abstract：:A viroid was isolated from symptomless Coleus blumei cultivar (cv.) 'Rainbow Gold' plants using the bidirectional PAGE method for analysis of small circular RNA molecules. The viriod was transmitted to viroid-free plants of Coleus blumei cv. 'Scarlet Dragonfly' by mechanical inoculation. Cloning and sequencing reveale...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718222
更新日期：1996-01-01 00:00:00
abstract：:Codiaeum variegatum (common name, garden croton) is an ornamental plant grown for its bright yellow variegated leaf morphology. Two C. variegatum plants with upward leaf curling and vein swelling symptoms were collected in Faisalabad, Pakistan. Sequencing of clones obtained by PCR amplification with specific primers s...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04633-3
更新日期：2020-08-01 00:00:00
abstract：:Highly purified virulent poliovirus preparations harbour an endogenous protein kinase. Enzyme activity increases significantly upon purification of infectious virus particles from infected HeLa cells. Enzyme activity is stimulated by divalent cations. The substrate specificity and the degree of stimulation of the kina...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311330
更新日期：1987-01-01 00:00:00
abstract：:Rabies virus (RABV) isolates from two species of canids and three species of bats were analyzed by comparing the C-terminal region of the G gene and the G-L intergenic region of the virus genome. Intercluster identities for the genetic sequences of the isolates showed both regions to be poorly conserved. Phylogenetic ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0671-7
更新日期：2010-06-01 00:00:00
abstract：:The betaherpesvirus human herpesvirus 6 (HHV-6) has two variants. The U83 gene product of strain HST is a chemoattractant for monocytes. Here, we describe U83 gene variations that accumulated in variants A and B. A gene-variation hot spot was examined in 36 different strains and one donor DNA sample. U83 gene variatio...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0307-3
更新日期：2009-01-01 00:00:00
abstract：:Coxsackievirus A8 (CV-A8), a member of the genus Enterovirus of the family Picornaviridae, can cause a variety of infectious diseases, such as hand, foot and mouth disease (HFMD), herpangina (HA), encephalitis, paralysis, myelitis, and meningitis. This is a first report of complete genome sequences of CV-A8 strains as...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2646-1
更新日期：2016-01-01 00:00:00
abstract：:By reverse transcription (RT)-nested polymerase chain reaction (PCR), hepatitis E virus (HEV) RNA was simultaneously detected in the livers of aborted fetuses and in fecal and serum samples from their sows, obtained from two of 14 farms tested. Sequence and phylogenetic analyses showed that these HEVs belonged to geno...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0679-z
更新日期：2010-07-01 00:00:00
abstract：:The nucleotide sequence of the 3' terminal region of the Australian isolate of papaya ringspot virus type W [PRSV-W (Aust)] was determined. An open reading frame (864 bp), encoding the putative coat protein gene, occurs upstream of the putative 3' untranslated region (206 bp) and poly(A) tail. A 23 amino acid sequence...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317141
更新日期：1992-01-01 00:00:00
abstract：:A full length cDNA copy of the genomic RNA of lettuce mosaic virus (LMV) was constructed under the control of an enhanced CaMV 35S promoter and of the NOS terminator. This construct was found infectious when inoculated to lettuce plants. The intron II of the bean nitrite reductase gene was engineered into the LMV FL c...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050474
更新日期：1998-01-01 00:00:00
abstract：:A total of 472 samples from domestic pigs collected in China from 2015 to 2018 were tested for the presence of porcine circovirus types 2 and 3 (PCV2 and PCV3, respectively) by conventional polymerase chain reaction analysis. The prevalence of PCV2, PCV3, and PCV2/3 co-infection was 50.0%, 13.3%, and 6.78%, respective...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04336-4
更新日期：2019-10-01 00:00:00
abstract：:Non-fatal rabies was successfully reproduced in rabbits infected intracerebrally with a highly pathogenic strain of street virus isolated from a man who had died of hydrophobia abter a dog bite and in white rats infected intracerebrally with the CVS strain of fixed virus. All the animals were pretreated with a subleth...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01318240
更新日期：1975-01-01 00:00:00
abstract：:Salmonella phages SenALZ1 and SenASZ3, two novel phages infecting Salmonella enterica, were isolated and analyzed. The genomes of these two phages consist of 154,811 and 157,630 base pairs (bp), with G+C contents of 44.56% and 44.74%, respectively. Fifty-nine of 199 open reading frames (ORFs) in the SenALZ1 genome, an...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04183-3
更新日期：2019-05-01 00:00:00
abstract：:Here, we report the molecular characterization of a novel partitivirus from Phomopsis vexans strain PvHZ002, a plant-pathogenic fungus infecting eggplant. The virus was designated "Phomopsis vexans partitivirus 1" (PvPV1). PvPV1 contains two dsRNA segments, dsRNA1 and dsRNA2, which are 1,662 bp and 1,628 bp long, resp...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04835-9
更新日期：2021-01-01 00:00:00
abstract：:In previous work, we demonstrated that the arenavirus Junín virus (JUNV) is able to activate Akt by means of the phosphatidylinositol-3-kinase (PI3K) survival pathway during virus entry. This work extends our study, emphasizing the relevance of this pathway in the establishment and maintenance of persistent infection ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2298-6
更新日期：2015-02-01 00:00:00
