Establishment of transformed swine fibroblast cell lines using SV40 large T antigen.

Abstract:

:Swine testicle cell lines were established by transformation of primary swine testicle (PST) cells with an SV40 plasmid (pSV3-neo), which contains genes conferring resistance to neomycin and expressing SV40 large T antigen. Plasmid DNA was transfected into PST cells using a lipofection system. Two related plasmids, pSV2-neo and pSV5-neo, failed to induce transformed cells. Cells transformed with pSV3-neo formed single colonies that were resistant to the antibiotic, G418, and expressed large T antigen. Upon two cycles of cloning by endpoint dilution method, three transformed clones, designated transformed swine testicle (tST)-3, tST-14 and tST-18, were selected and characterized in regards to cell replication and susceptibility to swine viruses. The resultant clones were compared with a counterpart non-transformed ST cell line (ATCC-ST). The three tST cell lines showed longer or the same doubling times and higher saturation densities compared to ATCC-ST cells. These cells were free from a range of adventitious agents and supported the replication of porcine parvovirus (PPV), pseudorabies virus (PRV) and transmissible gastroenteritis virus (TGEV), comparable to ATCC-ST cells. All three cell lines have been maintained in continuous cultures for over 60 passages with no changes in growth characteristics. These findings indicate that lipofection with pSV3-neo is an efficient means for the introduction of exogenous DNA into porcine cells and for establishment of transformed immortalized cell lines.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Choi CS,Murtaugh MP,Molitor TW

doi

10.1007/BF01310532

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

227-37

issue

3-4

eissn

0304-8608

issn

1432-8798

journal_volume

115

pub_type

杂志文章
  • Protective efficacy in mice of a secreted form of recombinant dengue-2 virus envelope protein produced in baculovirus infected insect cells.

    abstract::We constructed a recombinant baculovirus encoding a dengue (DEN)-2 virus envelope glycoprotein truncated of 102 amino acids (aa) at its C-terminus (D2E delta 102). The production, processing and transportation of the recombinant protein in baculovirus-infected Spodoptera frugiperda (Sf9) cells and its immunogenic prop...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01309465

    authors: Delenda C,Frenkiel MP,Deubel V

    更新日期:1994-01-01 00:00:00

  • Analysis of vaccine-virus-associated rabies cases in red foxes (Vulpes vulpes) after oral rabies vaccination campaigns in Germany and Austria.

    abstract::To eradicate rabies in foxes, almost 97 million oral rabies vaccine baits have been distributed in Germany and Austria since 1983 and 1986, respectively. Since 2007, no terrestrial cases have been reported in either country. The most widely used oral rabies vaccine viruses in these countries were SAD (Street Alabama D...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-009-0408-7

    authors: Müller T,Bätza HJ,Beckert A,Bunzenthal C,Cox JH,Freuling CM,Fooks AR,Frost J,Geue L,Hoeflechner A,Marston D,Neubert A,Neubert L,Revilla-Fernández S,Vanek E,Vos A,Wodak E,Zimmer K,Mettenleiter TC

    更新日期:2009-01-01 00:00:00

  • Phylogenetic tracking of current porcine epidemic diarrhea virus (PEDV) strains in the Philippines.

    abstract::To trace the possible route of introduction of porcine epidemic diarrhea virus (PEDV), the phylogenetic relationships of PEDV strains in the regions of epidemicity in the Philippines to PEDV strains that are endemic in other countries were investigated. Partial nucleotide sequences of the S1 spike gene was determined ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-016-2938-0

    authors: Paraguison-Alili R,Domingo CY

    更新日期:2016-09-01 00:00:00

  • Hepatitis C virus infection among illicit drug users in an archipelago of the Amazon.

    abstract::Hepatitis C virus (HCV) infection causes acute and chronic liver disease and may lead to cirrhosis, liver failure or hepatocellular carcinoma. The behavior of illicit drug users (DUs) typically exposes them to risks of viral infection. In the Brazilian Amazon region, a number of studies have identified high rates of d...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-017-3656-y

    authors: Silva FQ,Santos FJA,Andrade AP,Pacheco SDB,Fischer B,Pinho JRR,Lemos JAR,Oliveira-Filho AB

    更新日期:2018-03-01 00:00:00

  • Antigenic comparison of swine influenza virus isolates.

    abstract::Cross hemagglutination inhibition (HI) and neuraminidase inhibition (NI) tests demonstrated strong antigenic relationship between A/Swine/Wisconsin/1/73 (SW/73) and A/Swine/Shope/15/31 (SW/31) influenza viruses. An eigthyone fold purification of virus was achieved by adsorption and elution followed by differential ult...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01317967

    authors: Nath DM,Rodkey LS,Minocha HC

    更新日期:1975-01-01 00:00:00

  • Characterisation of a new potyvirus species infecting meadow saffron Colchicum autumnale).

    abstract::The alkaloids contained in Colchicum autumnale seeds are used in numerous medicines. Good quality seeds are difficult to obtain from this undomesticated plant. Therefore, a research program was set up aiming to cultivate C. autumnale in order to improve alkaloid contents and seed yields. In this context, a collection ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-004-0307-x

    authors: Poutaraud A,Desbiez C,Lemaire O,Lecoq H,Herrbach E

    更新日期:2004-07-01 00:00:00

  • Molecular detection of genotype 3 porcine hepatitis E virus in aborted fetuses and their sows.

    abstract::By reverse transcription (RT)-nested polymerase chain reaction (PCR), hepatitis E virus (HEV) RNA was simultaneously detected in the livers of aborted fetuses and in fecal and serum samples from their sows, obtained from two of 14 farms tested. Sequence and phylogenetic analyses showed that these HEVs belonged to geno...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-010-0679-z

    authors: Hosmillo M,Jeong YJ,Kim HJ,Park JG,Nayak MK,Alfajaro MM,Collantes TM,Park SJ,Ikuta K,Yunoki M,Kang MI,Park SI,Cho KO

    更新日期:2010-07-01 00:00:00

  • Efficacy of quantitative RT-PCR for detection of the nucleoprotein gene from different porcine rubulavirus strains.

    abstract::Blue-eye disease is an emergent viral swine infection caused by porcine rubulavirus (PoRV). We have developed a qRT-PCR method to detect and quantify expression of the nucleoprotein gene for different PoRV strains. The limit of detection for this assay was 10(2) copies of synthetic RNA. Viral RNA from PoRV was detecta...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-013-1672-0

    authors: Rivera-Benitez JF,García-Contreras Adel C,Reyes-Leyva J,Hernández J,Sánchez-Betancourt JI,Ramírez-Mendoza H

    更新日期:2013-09-01 00:00:00

  • An outbreak of echovirus 33 in schools in China in 2013.

    abstract::Echovirus 33 (E33) has been infrequently detected and is less frequently associated with clinical diseases when compared with other types of enteroviruses (EVs) in China. An outbreak of E33 was identified in four schools in Hunan Province, China, in June 2013. For laboratory diagnosis, throat swabs and/or serum specim...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-014-2059-6

    authors: Tan X,Gao L,Ma X,Nie J,Zhan D,Zhang B,Liu Y,Liu F,Xu W

    更新日期:2014-09-01 00:00:00

  • Molecular characterization of the hippeastrum chlorotic ringspot virus L segment and its protein.

    abstract::Hippeastrum chlorotic ringspot virus (HCRV) is a novel tospovirus that was identified in Yunnan Province, China, in 2013. We have sequenced the HCRV L gene, which is 8909 nt long and encodes the RNA-dependent RNA polymerase (2873 amino acids, 330.8 kDa). The HCRV L protein shared highest similarity (89.4 %) with that ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-014-2100-9

    authors: Xu Y,Lou SG,Li QF,Hu Q,Sun LP,Li ZY,Li YZ,Liu YT

    更新日期:2014-10-01 00:00:00

  • Development of a microchip electrophoresis-based, high-throughput PCR-RFLP method to type Tax 233 variants of bovine leukemia virus in Japan.

    abstract::Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). We used microchip electrophoresis in combination with automatic image analysis to develop a novel high-throughput PCR-RFLP to type the gene sequences that encode BLV Tax 233. This method revealed that 233L-Tax is more prevalent than ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-020-04842-w

    authors: Ochiai C,Katagiri Y,Kobayashi S,Naitoh I,Yoneyama S,Tomita K,Dongze L,Hikono H,Murakami K

    更新日期:2020-12-01 00:00:00

  • Effect of gene constellation and postreassortment amino acid change on the phenotypic features of H5 influenza virus reassortants.

    abstract::Reassortants between a low-pathogenic avian influenza virus strain A/Duck/Primorie/2621/2001 (H5N2) and a high-yield human influenza virus strain A/Puerto Rico/8/34 (H1N1) were generated, genotyped and analyzed with respect to their yield in embryonated chicken eggs, pathogenicity for mice, and immunogenicity. A reass...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-006-0931-8

    authors: Rudneva IA,Timofeeva TA,Shilov AA,Kochergin-Nikitsky KS,Varich NL,Ilyushina NA,Gambaryan AS,Krylov PS,Kaverin NV

    更新日期:2007-01-01 00:00:00

  • Immunisation with DNA polynucleotides protects mice against lethal challenge with St. Louis encephalitis virus.

    abstract::In vivo transfection by intramuscular injection with plasmids expressing the immunogenic proteins of microbial pathogens has considerable potential as a vaccination strategy against many pathogens of both man and animals. Here we report that weanling mice given a single intramuscular injection of 50 micrograms of a pl...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01718332

    authors: Phillpotts RJ,Venugopal K,Brooks T

    更新日期:1996-01-01 00:00:00

  • Adoptive transfer of macrophages from adult mice reduces mortality in mice infected with human enterovirus 71.

    abstract::Human enterovirus 71 (EV71) causes hand, foot and mouth disease in children under 6 years of age, and the neurological complications of this virus can lead to death. Until now, no vaccines or drugs have been available for the clinical control of this epidemic. Macrophages can engulf pathogens and mediate a series of h...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-012-1495-4

    authors: Liu J,Li X,Fan X,Ma C,Qin C,Zhang L

    更新日期:2013-02-01 00:00:00

  • Sequence comparison and phylogenetic analysis of fish nodaviruses based on the coat protein gene.

    abstract::We have amplified by reverse transcription-polymerase chain reaction (RT-PCR) and sequenced a 605-bp fragment covering the variable region of the coat protein gene of fish nodaviruses infecting European sea bass, Dicentrarchus labrax (n = 24), and shi drum, Umbrina cirrosa (n = 2), in the Mediterranean basin. Nine new...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s007050170110

    authors: Dalla Valle L,Negrisolo E,Patarnello P,Zanella L,Maltese C,Bovo G,Colombo L

    更新日期:2001-01-01 00:00:00

  • Regulation of single-strand RNA synthesis of alfalfa mosaic virus in non-transgenic cowpea protoplasts by the viral coat protein.

    abstract::We have compared the RNA synthesis of alfalfa mosaic virus in complete (by RNAs 1, 2 and 3) and incomplete infections (by RNAs 1 and 2) of cowpea protoplasts. Both viral RNA polymerase activity and accumulation of viral RNA were measured. By annealing RNA in solution with 32P-labelled probes of plus and minus polarity...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s007050050305

    authors: Houwing CJ,van de Putte P,Jaspars EM

    更新日期:1998-01-01 00:00:00

  • Epidermal hyperplasias of northern pike (Esox lucius) associated with herpesvirus and C-type particles.

    abstract::Northern pike from several locations in central Canada were observed to have two different types of hyperplastic epidermal lesions on the body and fins. One type of epidermal hyperplasia consisted of flat lesions, bluish-white in color with a granular or "gritty" appearance. Histological examination of this granular l...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01310815

    authors: Yamamoto T,Kelly RK,Nielsen O

    更新日期:1984-01-01 00:00:00

  • Localization of an RNA-binding domain in the nucleocapsid protein of the coronavirus mouse hepatitis virus.

    abstract::The interaction between the nucleocapsid (N) protein of mouse hepatitis virus (MHV) and RNA was studied in an effort to define portions of the N molecule that participate in binding to RNA. N mRNAs transcribed from SP6 and T7 vectors were translated in a rabbit reticulocyte lysate. Analysis of synthesized N protein in...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01309634

    authors: Masters PS

    更新日期:1992-01-01 00:00:00

  • The complete sequence of a Spanish isolate of Broad bean wilt virus 1 (BBWV-1) reveals a high variability and conserved motifs in the genus Fabavirus.

    abstract::The genome of a Spanish isolate of Broad bean wilt virus-1 (BBWV-1) was completely sequenced and compared with available sequences of other isolates of the genus Fabavirus (BBWV-1 and BBWV-2). This consisted of two RNAs of 5814 and 3431 nucleotides, respectively, and their organization was similar to that of other mem...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-005-0582-1

    authors: Ferrer RM,Guerri J,Luis-Arteaga MS,Moreno P,Rubio L

    更新日期:2005-10-01 00:00:00

  • Molecular characterization of a novel double-stranded RNA mycovirus of Trichoderma asperellum strain JLM45-3.

    abstract::In this study, we describe a novel mycovirus isolated from Trichoderma asperellum, which was designated Trichoderma asperellum dsRNA Virus 1 (TaRV1). The sequence analysis revealed that TaRV1 has two discontinuous open reading frames (ORF), ORF1 and ORF2. A hypothetical protein and an RNA-dependent RNA polymerase are ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-018-3988-2

    authors: Zhang T,Zeng X,Cai X,Liu H,Zeng Z

    更新日期:2018-12-01 00:00:00

  • A multigene analysis of the phylogenetic relationships among the flaviviruses (Family: Flaviviridae) and the evolution of vector transmission.

    abstract::The genus Flavivirus (family Flaviviridae) presently comprises around 70 single-strand positive-sense RNA viruses. These replicate in a range of vertebrate and invertebrate cells and may be mosquito-borne, tick-borne or have no-known-vector. Since transmission mode correlates strongly with phylogeny, the flaviviruses ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-005-0626-6

    authors: Cook S,Holmes EC

    更新日期:2006-02-01 00:00:00

  • Molecular characterization of yam virus X, a new potexvirus infecting yams (Dioscorea spp) and evidence for the existence of at least three distinct potexviruses infecting yams.

    abstract::The genome of yam virus X (YVX), a new member of the genus Potexvirus from yam (Dioscorea trifida), was completely sequenced. Structural and phylogenetic analysis showed that the closest relative of YVX is nerine virus X. A prevalence study found YVX only in plants maintained in Guadeloupe and showed that it also infe...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-014-2211-3

    authors: Mambole IA,Bonheur L,Dumas LS,Filloux D,Gomez RM,Faure C,Lange D,Anzala F,Pavis C,Marais A,Roumagnac P,Candresse T,Teycheney PY

    更新日期:2014-12-01 00:00:00

  • Contact infection of mink with 5 subtypes of avian influenza virus. Brief report.

    abstract::Avian influenza viruses of H3N8, H11N4, H7N7, H8N4, and H5N3 infected mink by contact. ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01309274

    authors: Okazaki K,Yanagawa R,Kida H

    更新日期:1983-01-01 00:00:00

  • Engineering of alfalfa mosaic virus RNA 3 into an expression vector.

    abstract::RNA 3 of alfalfa mosaic virus (AMV) encodes the 5'-proximal movement protein (MP) gene and the 3'-proximal coat protein (CP) gene which is expressed from a subgenomic RNA. Several strategies were explored to use this RNA as a vector for expression of the green fluorescent protein (GFP) in Nicotiana tabaccum plants exp...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s007050170125

    authors: Sanchez-Navarro J,Miglino R,Ragozzino A,Bol JF

    更新日期:2001-01-01 00:00:00

  • Expression and characterization of HPV-16 L1 capsid protein in Pichia pastoris.

    abstract::Human papillomaviruses (HPVs) are responsible for the most common human sexually transmitted viral infections. Infection with high-risk HPVs, particularly HPV16, is associated with the development of cervical cancer. The papillomavirus L1 major capsid protein, the basis of the currently marketed vaccines, self-assembl...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-009-0484-8

    authors: Bazan SB,de Alencar Muniz Chaves A,Aires KA,Cianciarullo AM,Garcea RL,Ho PL

    更新日期:2009-01-01 00:00:00

  • Rotavirus serotypes and electropherotypes in Finland from 1986 to 1990.

    abstract::Four epidemic seasons of rotaviruses were studied in Helsinki during 1986-1990. This is the first Scandinavian study, where both electropherotypes and serotypes are determined. Out of 5316 fecal specimens 769 (14.5%) rotavirus positive samples were detected by electron microscopy. Of these, 645 isolates (83.9%) gave a...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01314964

    authors: Maunula L,von Bonsdorff CH

    更新日期:1995-01-01 00:00:00

  • The enhancing effect of copper, nickel, and cobalt ions on plaque formation by Semliki Forest virus (SFV) in chicken embryo fibroblasts.

    abstract::In cultured chicken embryo fibroblasts treated with copper-(copper cation 10-minus 3.5-10-minus 5 M), nickel and cobalt salts an enhancement of the plating efficiency of Semliki Forest virus could be observed. This augmented plaque formation was most probably due to a higher adsorption rate of virions to the cell surf...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01315593

    authors: Wyler R,Wiesendanger W

    更新日期:1975-01-01 00:00:00

  • Nucleotide sequence and infectious transcripts from a full-length cDNA clone of the carmovirus Melon necrotic spot virus.

    abstract::We have studied the biological and molecular characteristics of a MNSV isolate collected in Spain (MNSV-Malpha5) and generated a full-length cDNA clone from which infectious RNA transcripts can be produced. The host range of MNSV-Malpha5 appeared to be limited to cucurbits and did not differ from that of MNSV-Dutch [4...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-002-0927-y

    authors: Díaz JA,Bernal JJ,Moriones E,Aranda MA

    更新日期:2003-03-01 00:00:00

  • Influenza A/Fujian/411/02(H3N2)-lineage viruses in Finland: genetic diversity, epidemic activity and vaccination-induced antibody response.

    abstract::The first sporadic cases of Fujian/411/02-lineage viruses were recorded in Finland in winter 2001-2002. The first protracted but low-intensity outbreak occurred here during the first half of 2003, and the second outbreak early in autumn 2003, after detection of sporadic influenza A cases in the summer. The calculated ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-005-0635-5

    authors: Ikonen N,Pyhälä R,Toivonen M,Korpela H

    更新日期:2006-02-01 00:00:00

  • Biosynthesis of measles virus hemagglutinin in persistently infected cells.

    abstract::The synthesis of the hemagglutinin (HA) glycoprotein of measles virus was investigated in a persistently infected cell line using a monoclonal anti-HA. The synthesis of the HA protein was shown to be associated with the rough endoplasmic reticulum. The unglycosylated (HA0) apoprotein is synthesized as a 65,000 dalton ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01314129

    authors: Bellini WJ,Silver GD,McFarlin DE

    更新日期:1983-01-01 00:00:00