Transgenic tobacco expressing geminiviral RNAs are resistant to the serious viral pathogen causing cotton leaf curl disease.

Abstract:

:Cotton, the major cash crop in Pakistan, suffers 30% losses to cotton leaf curl disease, caused by the geminivirus, cotton leaf curl virus DNA A, plus a satellite component, DNA beta responsible for symptom development with plants failing to produce cotton bolls. We constructed transgenic tobacco expressing sense and antisense RNAs representing: [i] the 5' half of the viral DNA replication gene, AC1, [ii] the 3' half of AC1, [iii] two overlapping genes, AC2, a transcription activator, and AC3, a replication enhancer. In contrast to controls, 25% of 72 transgenic tobacco lines tested showed heritable resistance [T(1) - T(3) generations]: symptom-free and no replication of DNA A or DNA beta even after 120 days of continuous exposure to viruliferous whiteflies. As geminiviral and transgene RNAs are not detected in resistant lines following infection, and selected uninfected resistant tobacco sense lines reveal double-stranded and small interfering RNAs, the most likely mechanism is via post-transcriptional gene silencing.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Asad S,Haris WA,Bashir A,Zafar Y,Malik KA,Malik NN,Lichtenstein CP

doi

10.1007/s00705-003-0179-5

keywords:

subject

Has Abstract

pub_date

2003-12-01 00:00:00

pages

2341-52

issue

12

eissn

0304-8608

issn

1432-8798

journal_volume

148

pub_type

杂志文章
  • Improved pig donor screening including newly identified variants of porcine endogenous retrovirus-C (PERV-C).

    abstract::Porcine endogenous retroviruses (PERV) are widely distributed in the genomes of pigs. PERV-A and PERV-B are present in all pigs. They infect human cells in vitro and therefore represent a risk for xenotransplantation when pig cells, tissues or organs are used. PERV-C infects only pig cells and is not present in the ge...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-012-1490-9

    authors: Kaulitz D,Mihica D,Adlhoch C,Semaan M,Denner J

    更新日期:2013-02-01 00:00:00

  • Physical mapping and field inversion gel electrophoresis of Amsacta moorei entomopoxvirus DNA.

    abstract::Agarose in situ digestion was used to prepare intact Amsacta moorei entomopoxvirus (AmEPV) DNA from embedded occlusion bodies (OBs). Direct dissolution of OBs in agarose eliminated the necessity for separate purification of virions. A physical map of AmEPV DNA was constructed for five restriction enzymes (BamHI, EcoRI...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01310704

    authors: Hall RL,Hink WF

    更新日期:1990-01-01 00:00:00

  • Absence of neuraminidase from influenza C virus.

    abstract::Influenza C viruses did not possess neuraminidase activity when examined using either fetuin or sialyllactose as substrate. Purified preparations of influenza C virus inhibited hemagglutination by NWS hemagglutinin. The hemagglutination inhibiting activity was abolished by treatment of influenza C virus with neuramini...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01320578

    authors: Nerome K,Ishida M,Nakayama M

    更新日期:1976-01-01 00:00:00

  • Role of the UL28 homologue of bovine herpesvirus 1 in viral DNA cleavage and packaging.

    abstract::In the aim to study the function of the bovine herpesvirus 1 (BoHV-1) UL28 protein during the replicative cycle, we characterized a UL28 deletion mutant of BoHV-1, BoHV-1 Delta UL28. Productive growth of BoHV-1 Delta UL28 was only observed in a specifically engineered complementing cell line expressing the native UL28...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-002-0962-8

    authors: Desloges N,Simard C

    更新日期:2003-04-01 00:00:00

  • Non-A, non-B hepatitis: visualization of virus-like particles from chimpanzee and human sera.

    abstract::Virus-like particles (VLP), morphologically similar to togavirus particles, were detected in the sera of chimpanzees and humans with chronic non-A, non-B (NANB) hepatitis. Particles resembling the virus were also found in the cytoplasm of hepatocytes obtained from NANB hepatitis infected chimpanzees. Furthermore, two ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01315557

    authors: Abe K,Kurata T,Shikata T

    更新日期:1989-01-01 00:00:00

  • Computational analysis of the effect of polymerase acidic (PA) gene mutation F35L in the 2009 pandemic influenza A (H1N1) virus on binding aspects of mononucleotides in the endonuclease domain.

    abstract::An F35L mutation in the N-terminal domain of the polymerase acidic protein (PA-Nter), which contains the active site of the endonuclease, has been reported to result in higher polymerase activity in mouse-adapted strains of the 2009 pandemic influenza A H1N1 virus. We modeled wild and mutant complexes of uridine 5'-mo...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-017-3681-x

    authors: Bhoye D,Cherian SS

    更新日期:2018-04-01 00:00:00

  • Inactivation of hepatitis C virus cDNA transgene by hypermethylation in transgenic mice.

    abstract::Transgenic mice were produced by microinjection of a partial hepatitis C virus (HCV) genome sequence including the structural protein region, under the control of the albumin promoter and enhancer into fertilized eggs of C57BL/6 and BDF1 mice. Three founders carrying at least five copies of the transgene but not expre...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01718169

    authors: Kato T,Ahmed M,Yamamoto T,Takahashi H,Oohara M,Ikeda T,Aida Y,Katsuki M,Arakawa Y,Shikata T,Esumi M

    更新日期:1996-01-01 00:00:00

  • Isolation of influenza A(H3N2)v virus from pigs and characterization of its biological properties in pigs and mice.

    abstract::Recently, a novel reassortant virus, influenza A(H3N2)v [A(H3N2)v], was identified as the causative pathogen in 307 human cases of influenza in the United States. A(H3N2)v contains the matrix gene from the 2009 pandemic H1N1 (pH1N1) virus, while its other genes originate from H3N2 viruses with triple-reassorted intern...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-012-1571-9

    authors: Kim SH,Kim HJ,Jin YH,Yeoul JJ,Lee KK,Oem JK,Lee MH,Park CK

    更新日期:2013-11-01 00:00:00

  • Nucleotide sequence of segment S9 of the rice dwarf virus genome.

    abstract::The complete nucleotide sequence of genome segment S9, which was reported as the ninth largest among the 12 genome segments of rice dwarf virus, was determined. The segment was 1,305 bp long and coded for a protein composed of 351 amino acids (Mr 38,930). The terminal sequences of the segment were found to consist of ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01313885

    authors: Fukumoto F,Omura T,Minobe Y

    更新日期:1989-01-01 00:00:00

  • Isolation, molecular characterization and evaluation of the pathogenicity of a porcine rotavirus isolated from Jiangsu Province, China.

    abstract::In this study, a porcine rotavirus was isolated from a fecal sample from a diarrheic piglet in Jiangsu Province, China. Rotavirus-specific cytopathic effects were observed after 12 blind passages on MA-104 cells, yielding a virus titer of 10(6.125) TCID50/ml. By applying an 80 % nucleotide cutoff value and the RotaC(2...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-015-2347-9

    authors: Zhang H,Zhang Z,Wang Y,Wang X,Xia M,Wu H

    更新日期:2015-05-01 00:00:00

  • Neutralizing activity of the antibodies against two kinds of envelope glycoproteins of Sendai virus.

    abstract::Murine monoclonal antibodies against the fusion (F) and hemagglutinin-neuraminidase (HN) proteins of Sendai virus (SV) were prepared and studied on their antiviral activities, particularly on the neutralization of infectivity. On the analysis with solid phase competitive ELISA, 26 anti-HN antibodies were divided into ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01316735

    authors: Tozawa H,Komatsu H,Ohkata K,Nakajima T,Watanabe M,Tanaka Y,Arifuku M

    更新日期:1986-01-01 00:00:00

  • Phylogenetic and geographic analysis of fowl adenovirus field strains isolated from poultry in Poland.

    abstract::Fowl adenoviruses (FAdVs) are widely distributed in chickens in Poland and throughout the world. FAdV infections have been reported in the United States, Australia, Europe, and the Mediterranean basin. Detection of FAdVs strains is very important from the epidemiological point of view and for monitoring disease outbre...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-015-2635-4

    authors: Niczyporuk JS

    更新日期:2016-01-01 00:00:00

  • Suspension culture process for H9N2 avian influenza virus (strain Re-2).

    abstract::H9N2 avian influenza virus has caused huge economic loss for the Chinese poultry industry since it was first identified. Vaccination is frequently used as a control method for the disease. Meanwhile suspension culture has become an important tool for the development of influenza vaccines. To optimize the suspension cu...

    journal_title:Archives of virology

    pub_type: 临床试验,杂志文章

    doi:10.1007/s00705-017-3460-8

    authors: Wang H,Guo S,Li Z,Xu X,Shao Z,Song G

    更新日期:2017-10-01 00:00:00

  • A human papillomavirus 8 E7 protein produced in plants is able to trigger the mouse immune system and delay the development of skin lesions.

    abstract::We investigated the potential of Nicotiana benthamiana to express the E7 protein of human papillomavirus 8 (HPV-8), a paradigm genotype among cutaneous HPVs. The protein, modified in its putative pRb-binding domain (E7(QGD)), was transiently expressed in leaves following infiltration with agrobacteria carrying either ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-010-0893-8

    authors: Noris E,Poli A,Cojoca R,Rittà M,Cavallo F,Vaglio S,Matic S,Landolfo S

    更新日期:2011-04-01 00:00:00

  • Detection of a novel border disease virus subgroup in Tunisian sheep.

    abstract::Nine pestiviruses isolated from different batches of a contaminated Tunisian sheep pox vaccine and one Tunisian field ovine isolate of border disease virus (BDV) were studied at the antigenic and molecular levels. Seroneutralization tests were carried out on three vaccine isolates, the Tunisian field isolate and repre...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-004-0427-3

    authors: Thabti F,Letellier C,Hammami S,Pépin M,Ribière M,Mesplède A,Kerkhofs P,Russo P

    更新日期:2005-02-01 00:00:00

  • Rapid detection of orf virus by loop-mediated isothermal amplification based on the DNA polymerase gene.

    abstract::At present, there are no effective antiviral treatments available for contagious ecthyma, and rapid diagnosis is therefore critical for effective control of the disease. Recently, the invention of a novel LAMP technique that can rapidly amplify nucleic acids with high specificity and sensitivity under isothermal condi...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-012-1526-1

    authors: Li J,Song D,He W,Bao Y,Lu R,Su G,Wang G,Lu H,Zhao K,Gao F

    更新日期:2013-04-01 00:00:00

  • Abortive infection with human cytomegalovirus induces an alteration of growth pattern: morphological changes with cytocidal effect in rabbit kidney epithelial cells. Brief report.

    abstract::Rabbit kidney epithelial cells (RK13) exhibited a cytopathic effect (CPE) characterized by cell rounding after infection with human cytomegalovirus (HCMV). Although HCMV-specific immediate early and early antigens were detected by indirect immunofluorescence techniques, neither late antigens nor infectious progeny vir...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01314121

    authors: Kamiya S,Tanaka J,Ogura T,Sato H,Ogura H,Yoshie T,Hatano M

    更新日期:1985-01-01 00:00:00

  • Pathogenicity and phylogenetic evaluation of the variant Newcastle disease viruses termed "pigeon PMV-1 viruses" based on the nucleotide sequence of the fusion protein gene.

    abstract::The nucleotide sequences of the entire F genes of two isolates of the pigeon PMV-1 (PPMV-1) variant of Newcastle disease virus (NDV) were determined using RTPCR. The deduced amino acid sequences of the F0 protein showed four differences between isolate 760/83 which had been passaged 4 times in chickens and gave an int...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01718322

    authors: Collins MS,Strong I,Alexander DJ

    更新日期:1996-01-01 00:00:00

  • Konjak mosaic virus: the complete nucleotide sequence of the genomic RNA and its comparison with other potyviruses.

    abstract::Konjak mosaic virus (KoMV) belongs to the genus Potyvirus, family Potyviridae. The complete nucleotide sequence of KoMV F isolate (KoMV F) was determined. The genome is 9,544 nucleotides long excluding the 3' terminal poly A tail and encodes a typical potyviral 350-kDa polyprotein of 3,087 amino acids. Phylogenetic an...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-006-0735-x

    authors: Nishiguchi M,Yamasaki S,Lu XZ,Shimoyama A,Hanada K,Sonoda S,Shimono M,Sakai J,Mikoshiba Y,Fujisawa I

    更新日期:2006-08-01 00:00:00

  • Characterization and identification of Oya virus, a Simbu serogroup virus of the genus Bunyavirus, isolated from a pig suspected of Nipah virus infection.

    abstract::A virus, named Oya virus, was isolated in Vero cell cultures from the lungs of a pig suspected of Nipah virus infection. The virus was revealed as a spherical enveloped RNA virus with a diameter of 79 nm. For identification of Oya virus, RT-PCR was performed. A common primer set for S-RNA of the Simbu serogroup of the...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-002-0838-y

    authors: Kono Y,Yusnita Y,Mohd Ali AR,Maizan M,Sharifah SH,Fauzia O,Kubo M,Aziz AJ

    更新日期:2002-08-01 00:00:00

  • Identification and characterization of a new virus in the genus Potyvirus from wild populations of Angelica lucida L. and A. genuflexa Nutt., family Apiaceae.

    abstract::A novel potyvirus was discovered in Angelica lucida L. (wild celery) and A. genuflexa Nutt. (kneeling Angelica) (family Apiaceae) in the Matanuska- Susitna Valley, Alaska. The experimental plant host range of the virus included species in three families: Chenopodiaceae (Chenopodium amaranticolor Coste et Reyn and Chen...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-007-1003-4

    authors: Robertson NL

    更新日期:2007-01-01 00:00:00

  • Development of a direct blood-based PCR system to detect BLV provirus using CoCoMo primers.

    abstract::Bovine leukemia virus (BLV), the etiologic agent of enzootic bovine leucosis, has caused pandemic outbreaks worldwide. Because transcription of the BLV is quickly blocked after infection, detecting integrated provirus at host genome is an important method of identifying whether an animal is infected. The aim of the pr...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-016-2806-y

    authors: Takeshima SN,Watanuki S,Ishizaki H,Matoba K,Aida Y

    更新日期:2016-06-01 00:00:00

  • Sub-genomic replicons of Tick-borne encephalitis virus.

    abstract::We constructed three sub-genomic replicons of Tick-borne encephalitis virus (TBEV) (Oshima REP, Oshima REP-GFP and Oshima REP-Neo) by deleting genes coding for structural proteins without or with insertion of green fluorescent protein (GFP) or Neo genes, respectively. BHK cells transfected with Oshima REP expressed th...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-003-0262-y

    authors: Hayasaka D,Yoshii K,Ueki T,Iwasaki T,Takashima I

    更新日期:2004-06-01 00:00:00

  • Deletion of a decoy epitope in porcine circovirus 2 (PCV2) capsid protein affects the protective immune response in mice.

    abstract::The swine pathogen porcine circovirus type 2 (PCV2) causes significant economic damage worldwide. The PCV2 capsid (CP) residues 169-STIDYFQPNNKR-180 have been identified as a decoy epitope that diverts the host immune response away from protective epitopes. However, the decoy epitope may include important linear or co...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-020-04831-z

    authors: Kim K,Shin M,Hahn TW

    更新日期:2020-12-01 00:00:00

  • Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan.

    abstract::Mycobacteriophage archival stocks have been kept for ca. 20-50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreov...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-018-3788-8

    authors: Ujihara T,Uchiyama J,Nasukawa T,Ando H,Murakami H,Ohara N,Ogawa M,Yamazaki T,Daibata M,Sakaguchi M,Matsuzaki S

    更新日期:2018-07-01 00:00:00

  • Complete genome sequence analysis of a genetic variant of grapevine virus L from the grapevine cultivar Blanc du Bois.

    abstract::The complete genome sequences of two grapevine virus L (GVL) isolates collected from the wine grape cultivar Blanc du Bois (Vitis spp.: 'Florida D 6-148'×'Cardinal') in Texas were determined. The two genome sequences (excluding the polyA tail) were each 7594 nucleotide long and 99.7% identical to each other, but they ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-020-04682-8

    authors: Alabi OJ,Appel DN,McBride S,Al Rwahnih M,Pontasch FM

    更新日期:2020-08-01 00:00:00

  • Expression of porcine Mx1 with FMDV IRES enhances the antiviral activity against foot-and-mouth disease virus in PK-15 cells.

    abstract::Foot-and-mouth disease virus (FMDV) is the most contagious pathogen in cloven-hoofed (two-toed) animals. Due to the rapid replication and spread of FMDV, novel therapeutic strategies are greatly needed to reduce or block FMDV shedding in cases of disease outbreak. Here, we generated an IRES-Mx1 construct in which the ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-015-2473-4

    authors: Yuan B,Fang H,Shen C,Zheng C

    更新日期:2015-08-01 00:00:00

  • Prediction of conserved microRNAs from skin and mucosal human papillomaviruses.

    abstract::Eight human papillomavirus (HPV) types including four cutaneous HPV types (HPV-5, HPV-8, HPV-20 and HPV-38) and four mucosal HPV types (HPV-6, HPV-11, HPV-16 and HPV-18) were selected for this miRNA study. Pre-miRNAs were predicted using a computer programme, and the conserved mature miRNAs were compared to currently ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-011-0974-3

    authors: Gu W,An J,Ye P,Zhao KN,Antonsson A

    更新日期:2011-07-01 00:00:00

  • Hepatitis C virus infection among illicit drug users in an archipelago of the Amazon.

    abstract::Hepatitis C virus (HCV) infection causes acute and chronic liver disease and may lead to cirrhosis, liver failure or hepatocellular carcinoma. The behavior of illicit drug users (DUs) typically exposes them to risks of viral infection. In the Brazilian Amazon region, a number of studies have identified high rates of d...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-017-3656-y

    authors: Silva FQ,Santos FJA,Andrade AP,Pacheco SDB,Fischer B,Pinho JRR,Lemos JAR,Oliveira-Filho AB

    更新日期:2018-03-01 00:00:00

  • Conservation of epitopes recognized by monoclonal antibodies against the separated subunits of influenza hemagglutinin among type A viruses of the same and different subtypes.

    abstract::Monoclonal antibodies raised against the separated hemagglutinin subunits (HA1 and HA2) of influenza A/Vic/3/75 (H3N2) virus were tested against a large panel of human and avian strains. The epitopes recognized by most antibodies were conserved among subtype H3 viruses, but reactivity of some antibodies with members o...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01319250

    authors: Sanchez-Fauquier A,Guillen M,Martin J,Kendal AP,Melero JA

    更新日期:1991-01-01 00:00:00