Abstract:
:Cotton, the major cash crop in Pakistan, suffers 30% losses to cotton leaf curl disease, caused by the geminivirus, cotton leaf curl virus DNA A, plus a satellite component, DNA beta responsible for symptom development with plants failing to produce cotton bolls. We constructed transgenic tobacco expressing sense and antisense RNAs representing: [i] the 5' half of the viral DNA replication gene, AC1, [ii] the 3' half of AC1, [iii] two overlapping genes, AC2, a transcription activator, and AC3, a replication enhancer. In contrast to controls, 25% of 72 transgenic tobacco lines tested showed heritable resistance [T(1) - T(3) generations]: symptom-free and no replication of DNA A or DNA beta even after 120 days of continuous exposure to viruliferous whiteflies. As geminiviral and transgene RNAs are not detected in resistant lines following infection, and selected uninfected resistant tobacco sense lines reveal double-stranded and small interfering RNAs, the most likely mechanism is via post-transcriptional gene silencing.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Asad S,Haris WA,Bashir A,Zafar Y,Malik KA,Malik NN,Lichtenstein CPdoi
10.1007/s00705-003-0179-5keywords:
subject
Has Abstractpub_date
2003-12-01 00:00:00pages
2341-52issue
12eissn
0304-8608issn
1432-8798journal_volume
148pub_type
杂志文章abstract::Porcine endogenous retroviruses (PERV) are widely distributed in the genomes of pigs. PERV-A and PERV-B are present in all pigs. They infect human cells in vitro and therefore represent a risk for xenotransplantation when pig cells, tissues or organs are used. PERV-C infects only pig cells and is not present in the ge...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1490-9
更新日期:2013-02-01 00:00:00
abstract::Agarose in situ digestion was used to prepare intact Amsacta moorei entomopoxvirus (AmEPV) DNA from embedded occlusion bodies (OBs). Direct dissolution of OBs in agarose eliminated the necessity for separate purification of virions. A physical map of AmEPV DNA was constructed for five restriction enzymes (BamHI, EcoRI...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310704
更新日期:1990-01-01 00:00:00
abstract::Influenza C viruses did not possess neuraminidase activity when examined using either fetuin or sialyllactose as substrate. Purified preparations of influenza C virus inhibited hemagglutination by NWS hemagglutinin. The hemagglutination inhibiting activity was abolished by treatment of influenza C virus with neuramini...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01320578
更新日期:1976-01-01 00:00:00
abstract::In the aim to study the function of the bovine herpesvirus 1 (BoHV-1) UL28 protein during the replicative cycle, we characterized a UL28 deletion mutant of BoHV-1, BoHV-1 Delta UL28. Productive growth of BoHV-1 Delta UL28 was only observed in a specifically engineered complementing cell line expressing the native UL28...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-002-0962-8
更新日期:2003-04-01 00:00:00
abstract::Virus-like particles (VLP), morphologically similar to togavirus particles, were detected in the sera of chimpanzees and humans with chronic non-A, non-B (NANB) hepatitis. Particles resembling the virus were also found in the cytoplasm of hepatocytes obtained from NANB hepatitis infected chimpanzees. Furthermore, two ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315557
更新日期:1989-01-01 00:00:00
abstract::An F35L mutation in the N-terminal domain of the polymerase acidic protein (PA-Nter), which contains the active site of the endonuclease, has been reported to result in higher polymerase activity in mouse-adapted strains of the 2009 pandemic influenza A H1N1 virus. We modeled wild and mutant complexes of uridine 5'-mo...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3681-x
更新日期:2018-04-01 00:00:00
abstract::Transgenic mice were produced by microinjection of a partial hepatitis C virus (HCV) genome sequence including the structural protein region, under the control of the albumin promoter and enhancer into fertilized eggs of C57BL/6 and BDF1 mice. Three founders carrying at least five copies of the transgene but not expre...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718169
更新日期:1996-01-01 00:00:00
abstract::Recently, a novel reassortant virus, influenza A(H3N2)v [A(H3N2)v], was identified as the causative pathogen in 307 human cases of influenza in the United States. A(H3N2)v contains the matrix gene from the 2009 pandemic H1N1 (pH1N1) virus, while its other genes originate from H3N2 viruses with triple-reassorted intern...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1571-9
更新日期:2013-11-01 00:00:00
abstract::The complete nucleotide sequence of genome segment S9, which was reported as the ninth largest among the 12 genome segments of rice dwarf virus, was determined. The segment was 1,305 bp long and coded for a protein composed of 351 amino acids (Mr 38,930). The terminal sequences of the segment were found to consist of ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01313885
更新日期:1989-01-01 00:00:00
abstract::In this study, a porcine rotavirus was isolated from a fecal sample from a diarrheic piglet in Jiangsu Province, China. Rotavirus-specific cytopathic effects were observed after 12 blind passages on MA-104 cells, yielding a virus titer of 10(6.125) TCID50/ml. By applying an 80 % nucleotide cutoff value and the RotaC(2...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2347-9
更新日期:2015-05-01 00:00:00
abstract::Murine monoclonal antibodies against the fusion (F) and hemagglutinin-neuraminidase (HN) proteins of Sendai virus (SV) were prepared and studied on their antiviral activities, particularly on the neutralization of infectivity. On the analysis with solid phase competitive ELISA, 26 anti-HN antibodies were divided into ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01316735
更新日期:1986-01-01 00:00:00
abstract::Fowl adenoviruses (FAdVs) are widely distributed in chickens in Poland and throughout the world. FAdV infections have been reported in the United States, Australia, Europe, and the Mediterranean basin. Detection of FAdVs strains is very important from the epidemiological point of view and for monitoring disease outbre...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2635-4
更新日期:2016-01-01 00:00:00
abstract::H9N2 avian influenza virus has caused huge economic loss for the Chinese poultry industry since it was first identified. Vaccination is frequently used as a control method for the disease. Meanwhile suspension culture has become an important tool for the development of influenza vaccines. To optimize the suspension cu...
journal_title:Archives of virology
pub_type: 临床试验,杂志文章
doi:10.1007/s00705-017-3460-8
更新日期:2017-10-01 00:00:00
abstract::We investigated the potential of Nicotiana benthamiana to express the E7 protein of human papillomavirus 8 (HPV-8), a paradigm genotype among cutaneous HPVs. The protein, modified in its putative pRb-binding domain (E7(QGD)), was transiently expressed in leaves following infiltration with agrobacteria carrying either ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0893-8
更新日期:2011-04-01 00:00:00
abstract::Nine pestiviruses isolated from different batches of a contaminated Tunisian sheep pox vaccine and one Tunisian field ovine isolate of border disease virus (BDV) were studied at the antigenic and molecular levels. Seroneutralization tests were carried out on three vaccine isolates, the Tunisian field isolate and repre...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0427-3
更新日期:2005-02-01 00:00:00
abstract::At present, there are no effective antiviral treatments available for contagious ecthyma, and rapid diagnosis is therefore critical for effective control of the disease. Recently, the invention of a novel LAMP technique that can rapidly amplify nucleic acids with high specificity and sensitivity under isothermal condi...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1526-1
更新日期:2013-04-01 00:00:00
abstract::Rabbit kidney epithelial cells (RK13) exhibited a cytopathic effect (CPE) characterized by cell rounding after infection with human cytomegalovirus (HCMV). Although HCMV-specific immediate early and early antigens were detected by indirect immunofluorescence techniques, neither late antigens nor infectious progeny vir...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314121
更新日期:1985-01-01 00:00:00
abstract::The nucleotide sequences of the entire F genes of two isolates of the pigeon PMV-1 (PPMV-1) variant of Newcastle disease virus (NDV) were determined using RTPCR. The deduced amino acid sequences of the F0 protein showed four differences between isolate 760/83 which had been passaged 4 times in chickens and gave an int...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718322
更新日期:1996-01-01 00:00:00
abstract::Konjak mosaic virus (KoMV) belongs to the genus Potyvirus, family Potyviridae. The complete nucleotide sequence of KoMV F isolate (KoMV F) was determined. The genome is 9,544 nucleotides long excluding the 3' terminal poly A tail and encodes a typical potyviral 350-kDa polyprotein of 3,087 amino acids. Phylogenetic an...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-006-0735-x
更新日期:2006-08-01 00:00:00
abstract::A virus, named Oya virus, was isolated in Vero cell cultures from the lungs of a pig suspected of Nipah virus infection. The virus was revealed as a spherical enveloped RNA virus with a diameter of 79 nm. For identification of Oya virus, RT-PCR was performed. A common primer set for S-RNA of the Simbu serogroup of the...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-002-0838-y
更新日期:2002-08-01 00:00:00
abstract::A novel potyvirus was discovered in Angelica lucida L. (wild celery) and A. genuflexa Nutt. (kneeling Angelica) (family Apiaceae) in the Matanuska- Susitna Valley, Alaska. The experimental plant host range of the virus included species in three families: Chenopodiaceae (Chenopodium amaranticolor Coste et Reyn and Chen...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-007-1003-4
更新日期:2007-01-01 00:00:00
abstract::Bovine leukemia virus (BLV), the etiologic agent of enzootic bovine leucosis, has caused pandemic outbreaks worldwide. Because transcription of the BLV is quickly blocked after infection, detecting integrated provirus at host genome is an important method of identifying whether an animal is infected. The aim of the pr...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2806-y
更新日期:2016-06-01 00:00:00
abstract::We constructed three sub-genomic replicons of Tick-borne encephalitis virus (TBEV) (Oshima REP, Oshima REP-GFP and Oshima REP-Neo) by deleting genes coding for structural proteins without or with insertion of green fluorescent protein (GFP) or Neo genes, respectively. BHK cells transfected with Oshima REP expressed th...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0262-y
更新日期:2004-06-01 00:00:00
abstract::The swine pathogen porcine circovirus type 2 (PCV2) causes significant economic damage worldwide. The PCV2 capsid (CP) residues 169-STIDYFQPNNKR-180 have been identified as a decoy epitope that diverts the host immune response away from protective epitopes. However, the decoy epitope may include important linear or co...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04831-z
更新日期:2020-12-01 00:00:00
abstract::Mycobacteriophage archival stocks have been kept for ca. 20-50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreov...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3788-8
更新日期:2018-07-01 00:00:00
abstract::The complete genome sequences of two grapevine virus L (GVL) isolates collected from the wine grape cultivar Blanc du Bois (Vitis spp.: 'Florida D 6-148'×'Cardinal') in Texas were determined. The two genome sequences (excluding the polyA tail) were each 7594 nucleotide long and 99.7% identical to each other, but they ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04682-8
更新日期:2020-08-01 00:00:00
abstract::Foot-and-mouth disease virus (FMDV) is the most contagious pathogen in cloven-hoofed (two-toed) animals. Due to the rapid replication and spread of FMDV, novel therapeutic strategies are greatly needed to reduce or block FMDV shedding in cases of disease outbreak. Here, we generated an IRES-Mx1 construct in which the ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2473-4
更新日期:2015-08-01 00:00:00
abstract::Eight human papillomavirus (HPV) types including four cutaneous HPV types (HPV-5, HPV-8, HPV-20 and HPV-38) and four mucosal HPV types (HPV-6, HPV-11, HPV-16 and HPV-18) were selected for this miRNA study. Pre-miRNAs were predicted using a computer programme, and the conserved mature miRNAs were compared to currently ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-0974-3
更新日期:2011-07-01 00:00:00
abstract::Hepatitis C virus (HCV) infection causes acute and chronic liver disease and may lead to cirrhosis, liver failure or hepatocellular carcinoma. The behavior of illicit drug users (DUs) typically exposes them to risks of viral infection. In the Brazilian Amazon region, a number of studies have identified high rates of d...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3656-y
更新日期:2018-03-01 00:00:00
abstract::Monoclonal antibodies raised against the separated hemagglutinin subunits (HA1 and HA2) of influenza A/Vic/3/75 (H3N2) virus were tested against a large panel of human and avian strains. The epitopes recognized by most antibodies were conserved among subtype H3 viruses, but reactivity of some antibodies with members o...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01319250
更新日期:1991-01-01 00:00:00