Abstract:
:Bovine leukemia virus (BLV), the etiologic agent of enzootic bovine leucosis, has caused pandemic outbreaks worldwide. Because transcription of the BLV is quickly blocked after infection, detecting integrated provirus at host genome is an important method of identifying whether an animal is infected. The aim of the present study was to develop a novel direct blood-based PCR system to detect the BLV provirus with high specificity and at low cost. The assay was based on the BLV-CoCoMo degenerate primers, which amplify all known BLV strains. Cattle blood samples (n = 182) were collected from the same BLV-positive farm and subjected to BLV-CoCoMo-direct-PCR to detect the BLV provirus. The proviral load was then estimated. This novel PCR method showed 100 % specificity. The BLV-CoCoMo-direct-PCR can be used in a variety of laboratory situations because it does not require expensive equipment/reagents, DNA purification, or a second round of PCR. Therefore, the method is extremely cost-effective and the risk of a false-positive result due to DNA contamination is very low.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Takeshima SN,Watanuki S,Ishizaki H,Matoba K,Aida Ydoi
10.1007/s00705-016-2806-ysubject
Has Abstractpub_date
2016-06-01 00:00:00pages
1539-46issue
6eissn
0304-8608issn
1432-8798pii
10.1007/s00705-016-2806-yjournal_volume
161pub_type
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