Abstract：
:A diagnostic assay to differentiate antibodies induced by foot-and-mouth disease virus (FMDV) infection from those induced by vaccination was developed. The test is an indirect-trapping ELISA which uses a monoclonal antibody to trap the non-structural 3ABC-FMDV polypeptide expressed in E. coli. Experimental and field sera from naive, vaccinated and infected cattle were examined. Using the established threshold of 0.20 optical density units, the sensitivity of the assay was 100%, as all the experimental post-infection sera (n degree = 137) gave values greater than this threshold, irrespective of the FMDV serotype used for the infection. In contrast, more than 99% of sera from vaccinated animals were negative (225 out of 228 primo-vaccinates and 159 out of 159 multi-vaccinates). A high degree of specificity was also confirmed by the finding that 99.5% (442 out of 444) of sera from naive animals gave negative results. Serum conversion against 3ABC was first detected 8 days post-infection and demonstrable levels of 3ABC specific antibodies were detectable at least 1 year post-infection. The described 3ABC-ELISA is safe, cheap and also easy to perform in large scale serological surveys. The high specificity and sensitivity makes this test an ideal tool for FMD eradication campaigns and control programs.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
De Diego M,Brocchi E,Mackay D,De Simone Fdoi
10.1007/s007050050219subject
Has Abstractpub_date
1997-01-01 00:00:00pages
2021-33issue
10eissn
0304-8608issn
1432-8798journal_volume
142pub_type
杂志文章abstract：:Chikungunya fever is self-limiting. However, neurological and hemorrhagic complications have been seen in recent outbreaks. The clinical manifestations of this disease are similar to those of dengue virus infection, indicating the need for differential diagnosis in areas such as India, which are endemic for both virus...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2829-4
更新日期：2016-06-01 00:00:00
abstract：:Blot hybridization analysis of infected bovine herpesvirus 1 (BHV-1) cellular RNA isolated at various times post infection and after treatment with specific metabolic inhibitors was used to characterize transcription of the BHV-1 Cooper isolate. Synthesis of BHV-1 RNA was detected as early as 3 h post infection and re...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01316744
更新日期：1991-01-01 00:00:00
abstract：:Multidrug-resistant uropathogenic Escherichia coli (UPEC) is increasing gradually on a worldwide scale. We therefore examined the possibility of bacteriophage (phage) therapy for urinary tract infections (UTIs) caused by the UPEC strains as an alternative to chemotherapy. In addition to the well-known T4 phage, KEP10,...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0031-4
更新日期：2008-01-01 00:00:00
abstract：:The C-terminal R peptide of ecotropic murine leukemia virus (MLV) envelope protein (Env) negatively controls membrane fusion activity. The R peptide cleavage during virion maturation activates its fusogenicity and is required for viral entry. We analyzed fusogenicity and transduction efficiency of mutant Env proteins ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-1054-6
更新日期：2007-01-01 00:00:00
abstract：:During the epidemic outbreak in the region of Greifswald in the winter 1974/75, we found influenza virus variants which showed differences in the electrophoretic mobility of HA. Among the 25 isolates 13 were of slower and 12 of higher mobility. HA1 of 6 isolates was studied by determining the number of the carbohydrat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310484
更新日期：1991-01-01 00:00:00
abstract：:Transmission electron microscopy was used to confirm the presence of picorna-like virus particles presumed to be Homalodisca coagulata virus-1 (HoCV-1) in the midgut region of adult glassy-winged sharpshooters (GWSS). In addition, we offer a reverse transcription polymerase chain reaction (RT-PCR) assay for the detect...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-1066-2
更新日期：2008-01-01 00:00:00
abstract：:Swine testicle cell lines were established by transformation of primary swine testicle (PST) cells with an SV40 plasmid (pSV3-neo), which contains genes conferring resistance to neomycin and expressing SV40 large T antigen. Plasmid DNA was transfected into PST cells using a lipofection system. Two related plasmids, pS...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310532
更新日期：1990-01-01 00:00:00
abstract：:The genomes of a large number of highly diverse novel circular DNA viruses from a wide range of sources have been characterised in recent years, including circular single-stranded DNA (ssDNA) viruses that share similarities with plant-infecting ssDNA viruses of the family Geminiviridae. Here, we describe six novel cir...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2358-6
更新日期：2015-05-01 00:00:00
abstract：:The genomes of all replication-competent retroviruses contain cis-acting elements that regulate gene expression. However, the identities of many of these elements remain to be characterized. Inserting sequences into the 3' untranslated region of a replication-competent spleen necrosis virus (SNV) vector disrupted its ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050486
更新日期：1999-01-01 00:00:00
abstract：:Trigeminal ganglia from swine latently infected with pseudorabies virus (PRV) were examined for the presence of PRV nucleic acids by in situ hybridization. Both viral DNA and RNA were detectable in latently infected neurons, indicating that the PRV genome is transcriptionally active during the latent phase of infectio...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01321010
更新日期：1988-01-01 00:00:00
abstract：:Classical swine fever (CSF), caused by the CSF virus (CSFV), is a highly contagious disease in pigs. In Korea, vaccination using a live-attenuated strain (LOM strain) has been used to control the disease. However, parenteral vaccination using a live-attenuated strain still faces a number of problems related to storage...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2182-4
更新日期：2014-12-01 00:00:00
abstract：:Rabbit kidney epithelial cells (RK13) exhibited a cytopathic effect (CPE) characterized by cell rounding after infection with human cytomegalovirus (HCMV). Although HCMV-specific immediate early and early antigens were detected by indirect immunofluorescence techniques, neither late antigens nor infectious progeny vir...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314121
更新日期：1985-01-01 00:00:00
abstract：:Atazanavir (ATZ) is a well-tolerated protease inhibitor that can be boosted with ritonavir (r) to treat infection with resistant strains of human immunodeficiency virus 1 (HIV-1). The aim of this meta-analysis was to compare the efficacy, safety, and metabolic effects of ATZ/r regimen versus commonly used antiretrovir...
journal_title：Archives of virology
pub_type： 杂志文章,meta分析,评审
doi：10.1007/s00705-017-3346-9
更新日期：2017-08-01 00:00:00
abstract：:Human papilloma virus DNA has been examined for heterogeneity by electron microscopy, reassociation kinetics, and restriction endonuclease digestion. No heterogeneity in contour length was detected. The reassociation rate of papilloma DNA isolated from pooled warts was consistent with that expected for a homogeneous, ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314794
更新日期：1977-01-01 00:00:00
abstract：:In this study, a porcine rotavirus was isolated from a fecal sample from a diarrheic piglet in Jiangsu Province, China. Rotavirus-specific cytopathic effects were observed after 12 blind passages on MA-104 cells, yielding a virus titer of 10(6.125) TCID50/ml. By applying an 80 % nucleotide cutoff value and the RotaC(2...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2347-9
更新日期：2015-05-01 00:00:00
abstract：:Viruses are ubiquitous in the biosphere and greatly affect the hosts they infect. It is generally accepted that members of every microbial taxon are susceptible to at least one virus, and a plethora of bacterial viruses are known. In contrast, knowledge of the archaeal virosphere is still limited. Here, a novel lytic ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-04120-w
更新日期：2019-03-01 00:00:00
abstract：:Genetic relationships among echovirus type 25 (ECV 25) isolates associated with aseptic meningitis in Germany 1997/98 and a 40-year-old ECV 25 prototype strain were investigated using RT-PCR and direct sequencing the VP1/2A gene junction region. Sequences were compared to each other and to non-polio enterovirus repres...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050640
更新日期：1999-01-01 00:00:00
abstract：:The complete bipartite genome (RNA1 and RNA2) of a new nepovirus infecting potato was obtained using small RNA sequencing and assembly complemented by Sanger sequencing. Each RNA encodes a single polyprotein, flanked by 5' and 3' untranslate regions (UTR) and followed by a poly (A) tail. The putative polyproteins enco...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3147-6
更新日期：2017-03-01 00:00:00
abstract：:Tryptic peptide analyses were performed on the major internal structural proteins of type D retroviruses isolated originally from subhuman primate species (MPMV, SMRV, LV) and from permanent human cell lines (HeLaV, HEp-2V, PMFV). The p25 peptide maps of MPMV, LV, PMFV and HEp-2V were very similar but showed a strikin...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309302
更新日期：1984-01-01 00:00:00
abstract：:Cucumber green mottle mosaic virus (CGMMV) is an important pathogen of cucumber (Cucumis sativus). The molecular mechanisms mediating host-pathogen interactions are likely to be strongly influenced by microRNAs (miRNAs), which are known to regulate gene expression during the disease cycle. This study focused on 14 miR...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04152-w
更新日期：2019-04-01 00:00:00
abstract：:In order to improve the knowledge of the humoral immune response to CMV infection, we developed an immunoblotting technique which allowed a better analysis of the changes in the pattern of anti CMV-polypeptides IgM. We examined 234 sera belonging to 27 renal allograft recipients developing a primary or recurrent CMV i...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310938
更新日期：1989-01-01 00:00:00
abstract：:The pathogenicity of 5 different encephalomyocarditis (EMC) virus isolates was investigated in swine fetuses following injection of each virus in utero. Laparotomies were performed on 3 pregnant sows in early mid-third (39-40 days) of gestation and on 5 sows in the late mid-third (70-72 days) of gestation, and groups ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310517
更新日期：1989-01-01 00:00:00
abstract：:The complete nucleotide sequence of the viral protein 2 (VP2) ORF was determined for 26 Vietnamese infectious bursal disease isolates collected from clinical outbreaks in vaccinated flocks from 1987 to 2018 and two commercial vaccine specimens. These sequences were compared for molecular classification with 42 referen...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04287-w
更新日期：2019-08-01 00:00:00
abstract：:Shorebirds on their southerly migration from Siberia to Australia, may pass through Asian regions currently experiencing outbreaks of highly pathogenic H5N1 influenza. To test for the presence of avian influenza viruses in migratory shorebirds arriving in Australia during spring 2004, 173 cloacal swabs were collected ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0784-1
更新日期：2006-11-01 00:00:00
abstract：:Genetically engineered expression of replicase encoding sequences has been proposed as an efficient system to confer protection against virus diseases by eliciting protection mechanisms in the plant. Potato leaf-roll was one of the first diseases for which this kind of protection was engineered in potato plants. Howev...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170095
更新日期：2001-07-01 00:00:00
abstract：:At low pH influenza virus causes membrane fusion. This phenomenon is thought to reflect a part of the infection mechanism of the virus. To obtain more information on the effect of low pH on the virus, the change in morphology of influenza virus particles was studied by electron microscopy. Further, the degradation of ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311162
更新日期：1984-01-01 00:00:00
abstract：:Norovirus (NoV) infection is the most common cause of nonbacterial acute gastroenteritis, which affects both adults and children. However, the molecular epidemiology of NoV in adults with acute gastroenteritis in China has not been investigated extensively. In this study, we investigated the occurrence of NoV infectio...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2440-0
更新日期：2015-07-01 00:00:00
abstract：:Three ponies were inoculated with plasma containing 10(4.8) TCID50 of equine infectious anemia virus (EIAV) and observed for 165 to 440 days. Each pony developed a febrile response within 3 weeks of infection during which a plasma viremia greater than or equal to 10(3.5) TCID50/ml was observed. Analyses of four isolat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311054
更新日期：1990-01-01 00:00:00
abstract：:Papillomavirus capsid proteins L1 and L2 mediate virion attachment, internalization and trafficking. In our studies of the capsid proteins, we identified an interaction of L2 with the E3 ligase Smad ubiquitin regulatory factor 2 (Smurf2). Smurf2 expression alters BPV1 virion trafficking and L2 protein levels. Using BP...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-0924-0
更新日期：2011-05-01 00:00:00
abstract：:Type I interferon (IFN) exerts strong antiviral activity, particularly against human immunodeficiency virus (HIV), and although several viral proteins have been shown to deregulate IFN induction, little is known about the induction of type I IFNs by HIV RNAs. In the present study, we used the stable isotope labeling w...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2372-8
更新日期：2015-05-01 00:00:00
