Abstract：
:Transmission electron microscopy was used to confirm the presence of picorna-like virus particles presumed to be Homalodisca coagulata virus-1 (HoCV-1) in the midgut region of adult glassy-winged sharpshooters (GWSS). In addition, we offer a reverse transcription polymerase chain reaction (RT-PCR) assay for the detection of this virus with a sensitivity of approximately 95 genome equivalents. A survey employing this assay in conjunction with GWSS samples collected throughout the United States including California, Hawaii, Florida Georgia, and the Carolinas revealed a fairly widespread pattern of distribution, although potentially restricted to temperate regions, areas with elevated host densities, or to populations of a common origin. The virus was found to naturally infect adults regardless of host plant and was not specific to a particular lifestage or sex. Examination of alternate leafhopper species further demonstrated that, although infection is not ubiquitous to all sharpshooter genera, HoCV-1 is not limited to Homalodisca vitripennis (=H. coagulata).
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Hunnicutt LE,Mozoruk J,Hunter WB,Crosslin JM,Cave RD,Powell CAdoi
10.1007/s00705-007-1066-2subject
Has Abstractpub_date
2008-01-01 00:00:00pages
61-7issue
1eissn
0304-8608issn
1432-8798journal_volume
153pub_type
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