Abstract：
:Multidrug-resistant uropathogenic Escherichia coli (UPEC) is increasing gradually on a worldwide scale. We therefore examined the possibility of bacteriophage (phage) therapy for urinary tract infections (UTIs) caused by the UPEC strains as an alternative to chemotherapy. In addition to the well-known T4 phage, KEP10, which was newly isolated, was used as a therapeutic phage candidate. KEP10 showed a broader bacteriolytic spectrum (67%) for UPEC strains than T4 (14%). Morphological and genetic analyses showed that KEP10 resembles phage T4. Phages T4 and KEP10 injected into the peritoneal cavity of mice were distributed immediately to all organs examined and maintained a high titer for at least 24 h. They were stable in the urine of both mice and humans for 24 h at 37 degrees C. Administration of these phages into the peritoneal cavity caused a marked decrease in the mortality of mice inoculated transurethrally with a UPEC strain, whereas most of the control mice died within a few days of bacterial infection. Inoculation with phage alone produced no adverse effects attributable to the phage per se. The present study experimentally demonstrated the therapeutic potential of phage for E. coli-induced UTIs, and T-even-related phages may be suitable candidates with which to treat them.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Nishikawa H,Yasuda M,Uchiyama J,Rashel M,Maeda Y,Takemura I,Sugihara S,Ujihara T,Shimizu Y,Shuin T,Matsuzaki Sdoi
10.1007/s00705-007-0031-4subject
Has Abstractpub_date
2008-01-01 00:00:00pages
507-15issue
3eissn
0304-8608issn
1432-8798journal_volume
153pub_type
杂志文章abstract：:Since serological donor-screening tests for HIV were introduced in 1985, the safety of donated blood components has improved dramatically. However, these tests do not completely prevent the risk of transfusion-associated HIV infection related to the use of blood donated during the pre-seroconversion window period. Tes...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1085-x
更新日期：2011-11-01 00:00:00
abstract：:The present study reports the first complete genome sequence analysis of West Nile virus (WNV) strains isolated from brain samples from raptors. The results prove the circulation of closely related WNV lineage II strains in central Europe and genetic analysis revealed seven amino acid substitutions in structural (PrM3...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-4056-7
更新日期：2019-01-01 00:00:00
abstract：:Rabbit anti-idiotypic antibodies (anti-ids) were generated against three bank vole and one human monoclonal antibody (MAb) specific for the two envelope glycoproteins of Puumala virus (G1 and G2). The anti-ids were purified by sequential immunoaffinity chromatography. Each anti-id inhibited the antigen binding of its ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309537
更新日期：1993-01-01 00:00:00
abstract：:We determined the complete genome sequence of the passion fruit woodiness virus Gld-1 isolate (PWV-Gld-1) from Australia and compared it with that of PWV-MU-2, another Australian isolate of PWV. The genomes shared high sequence identity in both the complete nucleotide sequence and the ORF amino acid sequence. All of t...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1670-2
更新日期：2013-08-01 00:00:00
abstract：:The objectives of this study were to determine the antigenic relationship among ruminant adenoviruses and determine their phylogenetic relationship based on the deduced hexon gene amino acid sequence. Results of reciprocal cross-neutralization tests demonstrated antigenic relationships in either one or both directions...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0063-4
更新日期：2008-01-01 00:00:00
abstract：:A group of previously isolated heterogeneous mEp lambdoid phages (43) from 19 different immunity groups for phage infection was further characterized to gain insight into some phenotypic traits and to assess their relationship with phage lambda. Interestingly, the FhuA host receptor was required by the majority of mEp...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0111-0
更新日期：2008-01-01 00:00:00
abstract：:Complete nucleotide sequences of three strains (I, III, and IV) of Barley yellow mosaic virus (BaYMV) isolated in Japan were determined. The length of the genome was the same among the three strains; RNA1 was 7,642 nt and RNA2 was 3,585 nt. The molecular phylogenetic analysis showed that strain I was most closely rela...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0163-1
更新日期：2008-01-01 00:00:00
abstract：:Hybridomas secreting monoclonal antibodies against herpes simplex virus (HSV) DNA binding proteins (DBP) have been produced. Five HSV DBP have been characterized according to molecular weight, affinity for DNA, kinetic class and localization within the infected cell. By preparing an immunoadsorbent column from antibod...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314300
更新日期：1984-01-01 00:00:00
abstract：:Viruses are ubiquitous in the biosphere and greatly affect the hosts they infect. It is generally accepted that members of every microbial taxon are susceptible to at least one virus, and a plethora of bacterial viruses are known. In contrast, knowledge of the archaeal virosphere is still limited. Here, a novel lytic ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-04120-w
更新日期：2019-03-01 00:00:00
abstract：:Mouse cell lines (L, NIH3T3, and RMA cells) infected with the Edmonston strain of measles virus (MV) did not exhibit cytopathic effects (CPE), consistent with the finding that mice are not susceptible to MV. Northern blot analysis, however, revealed that MV genes were transcribed in infected L and NIH3T3 cells, althou...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310037
更新日期：1994-01-01 00:00:00
abstract：:A putative novel positive-sense (+) RNA virus was detected in isolate CF16158 of the fungus Fusarium graminearum, the causal agent of Fusarium head blight and crown rot in wheat in China. The full genome of this virus was sequenced and characterized. The complete cDNA sequence is 7,051 nt long and contains four open r...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04486-5
更新日期：2020-02-01 00:00:00
abstract：:Truncated proteins corresponding to the C-terminal half of VP1 of four vaccine strains and two field variants of foot-and-mouth disease virus (FMDV) were expressed in E. coli. The expressed proteins were affinity purified and their type specific reactivity was confirmed by immunoprecipitation with anti-virus antibodie...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050698
更新日期：1999-01-01 00:00:00
abstract：:A number of avian avulavirus 1 (AAvV 1) isolates have been reported from avian and non-avian hosts worldwide with varying clinical consequences. In this regard, robust surveillance coupled with advanced diagnostics, genomic analysis, and disease modelling has provided insight into the molecular epidemiology and evolut...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04276-z
更新日期：2019-07-01 00:00:00
abstract：:Chrysanthemums worldwide suffer from a high incidence of infection with chrysanthemum virus B (CVB), a member of the genus Carlavirus, family Betaflexiviridae. Three major lineages or strains of this virus have been found in India, but none have been characterized beyond the genetic variation they display in their coa...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1190-x
更新日期：2012-03-01 00:00:00
abstract：:We previously generated an RCMV strain in which the r144 gene, encoding a major histocompatibility complex class I homolog, had been deleted (RCMVdelta r144). To investigate the role of r144 during acute infection of neonatal rats, we infected three days-old neonatal rats with either RCMVdelta r144 or wild type (wt) R...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050200028
更新日期：2002-04-01 00:00:00
abstract：:The basic structure of human immunodeficiency virus type 1 (HIV-1) has been investigated morphologically; however, the internal structure of HIV-1 core is not well understood. We studied the internal structures by transmission electron microscopy. We modified the method for electron staining of ultrathin sections and ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050083
更新日期：1997-01-01 00:00:00
abstract：:In a virus-infected plant, small interfering RNAs (siRNAs) corresponding to the viral genome form a large proportion of the small RNA population. It is possible to reassemble significant portions of the virus sequence from overlapping siRNA sequences and use these to identify the virus. We tested this technique with a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-0979-y
更新日期：2011-07-01 00:00:00
abstract：:Dengue virus is the most important arbovirus that affects humans, and it can establish persistent infections, especially in insect-derived cell cultures. Defective viral genomes have been implicated in the establishment and maintenance of persistent infections with several flaviviruses; however, there exists almost no...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1525-2
更新日期：2013-03-01 00:00:00
abstract：:Two cell lines, CNS-5 and CNS-6, were established by cocultivation of sedimented cells in cerebrospinal fluid (CSF) from two anti-human T-lymphotropic virus type I (HTLV-I) antibody-positive male patients with encephalopathy and HTLV-I-associated myelopathy, respectively, with peripheral blood mononuclear cells from a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311306
更新日期：1991-01-01 00:00:00
abstract：:The effect of the local anaesthetic drug procaine on the adsorption of two enveloped viruses was studied. Physiological concentrations of the drug (7 X 10(-3)--7 X 10(-2) M) strongly inhibited the adsorption of both West-Nile and herpes viruses as determined by plaque assay and the infective center assay. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317291
更新日期：1978-01-01 00:00:00
abstract：:Serologic and virologic prevalence of infection with different swine influenza virus (SIV) subtypes was investigated using swine sera, nasal swabs and lung samples that had been submitted for a diagnosis to the Minnesota Veterinary Diagnostic Laboratory. A total of 111,418 pig sera were tested for SIV antibody between...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0788-4
更新日期：2002-06-01 00:00:00
abstract：:A virus, named Oya virus, was isolated in Vero cell cultures from the lungs of a pig suspected of Nipah virus infection. The virus was revealed as a spherical enveloped RNA virus with a diameter of 79 nm. For identification of Oya virus, RT-PCR was performed. A common primer set for S-RNA of the Simbu serogroup of the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0838-y
更新日期：2002-08-01 00:00:00
abstract：:A physical map for the genome of Spodoptera littoralis (Egyptian cottonworm) nuclear polyhedrosis virus of B-type (S1MNPV-B) [Cherry CL, Summers MD (1985) J Invertebr Pathol 46: 289-295] was prepared using the restriction endonucleases SmaI, SfiI, NotI, ApaI, KpnI, BstEII, PstI, SacI, and HindIII. The size of the geno...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313815
更新日期：1989-01-01 00:00:00
abstract：:The nucleo-, phospho- and matrix protein of measles virus were localized at high resolution within infected cells by use of post-embedding immunogold labelling techniques. In general, labelling with monospecific antibodies as well as with a polyvalent rabbit anti-measles hyperimmune antiserum revealed measles virus po...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311011
更新日期：1990-01-01 00:00:00
abstract：:We constructed a recombinant baculovirus encoding a dengue (DEN)-2 virus envelope glycoprotein truncated of 102 amino acids (aa) at its C-terminus (D2E delta 102). The production, processing and transportation of the recombinant protein in baculovirus-infected Spodoptera frugiperda (Sf9) cells and its immunogenic prop...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309465
更新日期：1994-01-01 00:00:00
abstract：:Simian adenovirus SA7 heated for 30 minutes at 70 degrees C retained part of its ability to induce tumors in newborn hamsters. Tumors were also induced by DNA extracted from the heated virus. However, neither residual replicating, i.e. cytopathogenic, nor transforming and T-antigen-inducing activities could be detecte...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317334
更新日期：1981-01-01 00:00:00
abstract：:A novel virus we call zucchini green mottle mosaic virus (ZGMMV) was isolated from zucchini squash and its properties were determined. The size and shape of its virions, and other properties suggest that the virus is a tobamovirus. The coat protein (CP) genes of ZGMMV and kyuri green mottle mosaic virus (KGMMV), which...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070023
更新日期：2000-01-01 00:00:00
abstract：:Seven isolates of West Nile virus and an additional isolate tentatively identified at the outset as West Nile virus, were compared by Casals' modified haemagglutination inhibition technique. Two of the isolates were nearly identical; a second set of five isolates cross-reacted among themselves within a wide range and ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315279
更新日期：1978-01-01 00:00:00
abstract：:Type I interferon (IFN) exerts strong antiviral activity, particularly against human immunodeficiency virus (HIV), and although several viral proteins have been shown to deregulate IFN induction, little is known about the induction of type I IFNs by HIV RNAs. In the present study, we used the stable isotope labeling w...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2372-8
更新日期：2015-05-01 00:00:00
abstract：:The DNA of mammalian cells treated with 4,5',8-trimethylpsoralen (Trioxsalen) is crosslinked (preventing transcription) only after photoactivation with near ultraviolet light. Suppression of gene action in cocultivated or fused cultures, including both induction and antiviral action of fibroblast interferon, can conse...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320762
更新日期：1980-01-01 00:00:00
