Abstract:
:Dengue virus is the most important arbovirus that affects humans, and it can establish persistent infections, especially in insect-derived cell cultures. Defective viral genomes have been implicated in the establishment and maintenance of persistent infections with several flaviviruses; however, there exists almost no information concerning defective dengue virus genomes. Here, we report the detection of defective dengue 2 virus genomes in persistently infected mosquito C6/36 cells. The defective viral genomes were detected at a low ratio compared with the wild-type genome. Deletions of approximately 147 residues (222-368) were found in the E protein, and these mainly affected domain III (73 %) of the protein; deletions of approximately 153 residues (4-156) and 228 residues (597-825) were found in the methyltransferase and polymerase domains, respectively, of the NS5 protein. The truncated versions of NS5 could be detected by western blot only in the protein extracts derived from persistently infected cells.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Juárez-Martínez AB,Vega-Almeida TO,Salas-Benito M,García-Espitia M,De Nova-Ocampo M,Del Ángel RM,Salas-Benito JSdoi
10.1007/s00705-012-1525-2subject
Has Abstractpub_date
2013-03-01 00:00:00pages
583-99issue
3eissn
0304-8608issn
1432-8798journal_volume
158pub_type
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