Structure and genome organization of the large RNA of iris yellow spot virus (genus Tospovirus, family Bunyaviridae).

Abstract:

:The structure and organization of the large (L) RNA of iris yellow spot virus (IYSV) was determined, and with this report, the complete genomic sequence of IYSV of the genus Tospovirus, family Bunyaviridae has been elucidated. The L RNA of IYSV was 8,880 nucleotides in length and contained a single open reading frame in the viral complementary (vc) strand. The primary translation product of 331.17 kDa shared many of the features of the viral RNA-dependent RNA polymerase (RdRp) coded by L RNAs of known tospoviruses. The 5' and 3' termini of IYSV L RNA (vc) contain two untranslated regions of 33 and 226 nucleotides, respectively, and both termini have conserved terminal nucleotides, another common feature of tospovirus genomic RNAs. Conserved motifs characteristic of RdRps of members of the family Bunyaviridae were present in the IYSV RdRp.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Bag S,Druffel KL,Pappu HR

doi

10.1007/s00705-009-0568-5

subject

Has Abstract

pub_date

2010-02-01 00:00:00

pages

275-9

issue

2

eissn

0304-8608

issn

1432-8798

journal_volume

155

pub_type

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