Abstract:
:The full length gene for the coat protein (CP) of the potyvirus, Johnsongrass mosaic virus, was incorporated into recombinant baculovirus and expressed in insect cells. Western blot and Coomassie-stained polyacrylamide gel electrophoresis analysis of infected insect cells demonstrated that CP was produced in large quantity. Electron microscopic examination of these cells showed the presence of numerous potyvirus-like particles in the cytoplasm. Morphologically the particles resembled potyvirus particles assembled in vitro in the absence of viral RNA and those found in Escherichia coli expressing the recombinant CP gene.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Edwards SJ,Hayden MB,Hamilton RC,Haynes JA,Nisbet IT,Jagadish MNdoi
10.1007/BF01321065subject
Has Abstractpub_date
1994-01-01 00:00:00pages
375-80issue
3-4eissn
0304-8608issn
1432-8798journal_volume
136pub_type
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