Abstract：
:As a prerequisite for development of improved vaccines and diagnostic tools for control of the fish pathogen koi herpesvirus, or cyprinid herpesvirus 3 (CyHV-3), we have started to identify putative viral envelope and capsid proteins. The complete or partial CyHV-3 open reading frames ORF25, ORF65, ORF92, ORF99, ORF136, ORF138, ORF146, ORF148, and ORF149 were expressed as bacterial fusion proteins, which were then used for preparation of monospecific rabbit antisera. All of the sera that were obtained detected their target proteins in cells transfected with the corresponding eukaryotic expression plasmids. However, only the type I membrane proteins pORF25, pORF65, pORF99, pORF136 and pORF149 and the major capsid protein pORF92 were sufficiently abundant and immunogenic to permit unambiguous detection in CyHV-3-infected cells. In indirect immunofluorescence tests (IIFT), sera from naturally or experimentally CyHV-3-infected carp and koi predominantly reacted with cells transfected with expression plasmids encoding pORF25, pORF65, pORF148, and pORF149, which represent a family of related CyHV-3 membrane proteins. Moreover, several neutralizing monoclonal antibodies raised against CyHV-3 virions proved to be specific for pORF149 in IIFT of transfected cells and in immunoelectron microscopic analysis of CyHV-3 particles. Since pORF149 appears to be an immunorelevant envelope protein of CyHV-3, a recombinant baculovirus was generated for its expression in insect cells, and pORF149 was shown to be incorporated into pseudotyped baculovirus particles, which might be suitable as diagnostic tools or subunit vaccines.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Fuchs W,Granzow H,Dauber M,Fichtner D,Mettenleiter TCdoi
10.1007/s00705-014-2190-4subject
Has Abstractpub_date
2014-12-01 00:00:00pages
3257-68issue
12eissn
0304-8608issn
1432-8798journal_volume
159pub_type
杂志文章abstract：:The entry modes of Japanese encephalitis (JE) and dengue-2 (DEN-2) viruses into C6/36 mosquito cells and of DEN-2 virus into human peripheral blood monocytes in vitro were studied. Inoculation of either JE or DEN-2 virions into C6/36 cells resulted in direct penetration of the virions into the cytoplasm at the cell su...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313814
更新日期：1989-01-01 00:00:00
abstract：:The aim of this study was to develop and evaluate a two-tube multiplex real-time RT-PCR assay for the detection and identification of four viral hemorrhagic fever (VHF) pathogens, severe fever with thrombocytopenia syndrome virus (SFTSV), Hantaan virus (HTNV), Seoul virus (SEOV), and dengue virus (DENV), from human cl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1677-8
更新日期：2013-09-01 00:00:00
abstract：:Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae) infects plants of the genus Lilium, causing a reduction in flower and bulb quality. A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed to detect the coat protein gene of LMoV. This LAMP method was highly specific for LMo...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3618-4
更新日期：2018-02-01 00:00:00
abstract：:Human cytomegalovirus disappeared from cultures continuously exposed to interferon. When interferon was removed, high titers of virus reappeared. Cytomegalovirus was thus able to persist in potentially infectious form in cells protected by interferon. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309276
更新日期：1983-01-01 00:00:00
abstract：:Two day-old athymic (rnu/rnu) and euthymic (rnu/+) rat pups nursing immune or non-immune dams were inoculated oronasally with the Yale strain of rat virus (RV-Y). All athymic and euthymic pups (57/57) from immune dams remained clinically normal, whereas 51 of 66 athymic and euthymic pups from non-immune dams died with...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311299
更新日期：1991-01-01 00:00:00
abstract：:NDV variants released from the early passaged BHK21/WI-2 cells persistently infected with NDV carry the structural antigen of a latent virus of BHK21/WI-2 cells as a result of phenotypic mixing between NDV and the latent virus. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317290
更新日期：1978-01-01 00:00:00
abstract：:Pigeon circovirus (PiCV) is taxonomically classified as a member of the Circovirus genus, family Circoviridae. The virus contains a single stranded DNA genome of approximately 2 kb, with minor length variations among different isolates. The occurrence of PiCV infections in pigeons (Columba livia) has been documented w...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3990-8
更新日期：2018-11-01 00:00:00
abstract：:Helper component protease (HC-Pro) is a potyvirus-encoded multifunctional protein and a major determinant of symptom expression in a susceptible plant. Here, we show the involvement of clover yellow vein virus (ClYVV) HC-Pro in necrotic symptom expression in broad bean (Vicia faba cv. Wase). In this host, lethal necro...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-1073-3
更新日期：2008-01-01 00:00:00
abstract：:The use of the complete DNA sequence for the Marek's disease virus (MDV) serotype 1 vaccine strain CVI988 Rispens in comparative genomic studies with virulent strains of MDV has revealed the presence of a number of insertions, deletions and single-nucleotide polymorphisms. In this study, we investigated a SNP in the H...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0152-4
更新日期：2008-01-01 00:00:00
abstract：:An arracacha (Arracacia xanthorrhiza) plant collected in Brazil was found to be infected by a new virus. This viral isolate (named C17) systemically infected Nicotiana benthamiana and Apium graveolens. A polyclonal antibody was raised, and analysis of our arracacha germplasm collection showed a high infection rate of ...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-008-0289-1
更新日期：2009-01-01 00:00:00
abstract：:Monensin, at concentrations which depended on the multiplicity of infection, was found to prevent DNA replication of human cytomegalovirus (HCMV) as well as production of viral progeny in human foreskin fibroblasts. The drug did not affect DNA replication of herpes simplex virus. Inhibition of consecutive HCMV DNA syn...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310716
更新日期：1987-01-01 00:00:00
abstract：:Meningitis is a serious condition that affects the central nervous system. It is an inflammation of the meninges, which is the membrane that surrounds both the brain and the spinal cord. Meningitis can be caused by bacterial, viral, or fungal infections. Many viruses, such as enteroviruses, herpesviruses, and influenz...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-020-04891-1
更新日期：2021-01-03 00:00:00
abstract：:Evidence of type-C RNA viral activity in fetal hamster cells transformed in vitro by 1-B-D-arabinofuranosylcytosine (ara-C) after at least one in vivo passage is described. The virus possesses properties typical of other type-C RNA viruses, such as: a) morphology as determined with the electron microscope, b) presence...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317556
更新日期：1979-01-01 00:00:00
abstract：:The betaherpesvirus human herpesvirus 6 (HHV-6) has two variants. The U83 gene product of strain HST is a chemoattractant for monocytes. Here, we describe U83 gene variations that accumulated in variants A and B. A gene-variation hot spot was examined in 36 different strains and one donor DNA sample. U83 gene variatio...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0307-3
更新日期：2009-01-01 00:00:00
abstract：:The viral DNAs induced by the unclassified animal virus, chicken anaemia agent (CAA), during replication in MDCC-MSB 1 cells have been investigated. Analyses after S1 nuclease, restriction endonuclease and denaturation treatments indicated that infected cell extracts contained genome-size, single-stranded DNA (M(r) 2....
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309811
更新日期：1992-01-01 00:00:00
abstract：:One host polypeptide (40,000 daltons) synthesized prior to infection is associated with the 250S RNA polymerase structure partially purified by a combination of velocity sedimentation and isopycnic separation. A series of pulse-chase experiments have shown that a 56,000 dalton polypeptide made during the eclipse phase...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317808
更新日期：1975-01-01 00:00:00
abstract：:Dengue virus is the most important arbovirus that affects humans, and it can establish persistent infections, especially in insect-derived cell cultures. Defective viral genomes have been implicated in the establishment and maintenance of persistent infections with several flaviviruses; however, there exists almost no...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1525-2
更新日期：2013-03-01 00:00:00
abstract：:Bovine leukemia virus (BLV), the etiologic agent of enzootic bovine leucosis, has caused pandemic outbreaks worldwide. Because transcription of the BLV is quickly blocked after infection, detecting integrated provirus at host genome is an important method of identifying whether an animal is infected. The aim of the pr...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2806-y
更新日期：2016-06-01 00:00:00
abstract：:Classical swine fever (CSF), caused by the CSF virus (CSFV), is a highly contagious disease in pigs. In Korea, vaccination using a live-attenuated strain (LOM strain) has been used to control the disease. However, parenteral vaccination using a live-attenuated strain still faces a number of problems related to storage...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2182-4
更新日期：2014-12-01 00:00:00
abstract：:A persistent, defective infection by an unknown strain of Newcastle disease virus (NDV) appeared accidentally in established lines of pig, ox and sheep kidney cells. Virus particles released from the persistently infected cells were not infectious and were deficient in neuraminidase activity. Synthesis of some of the ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01318009
更新日期：1976-01-01 00:00:00
abstract：:The molecular epidemiology of a recent norovirus (NoV) outbreak in Brazil performed by comparative analysis with Genebank NoV sequences showed that the GII.4 strain was responsible for 72.5% of all NoV-positive cases (58/80). Other detected NoV strains included GII.3 (7/80; 8.8%) and GII.9 (8/80; 10%). This is the fir...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0078-x
更新日期：2008-01-01 00:00:00
abstract：:Cumulative studies have demonstrated that dengue virus infection results in the induction of apoptosis of certain cells in vitro. Moreover, apoptosis of microvascular endothelial cells in the brain and in the intestinal serosa has been demonstrated postmortem in dengue virus (DENV)-infected patients. In this work, hum...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0396-7
更新日期：2009-01-01 00:00:00
abstract：:We have prepared monoclonal antibodies to each of the enteric adenoviruses types 40 and 41. Three different hybridoma cell lines were selected which produced antibody found to react by radioimmunoprecipitation with adenovirus (Ad) hexon antigens. One was specific for Ad 40, another for Ad 41, and a third one reacted w...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310718
更新日期：1987-01-01 00:00:00
abstract：:A possible antigenic relationship between the porcine enteropathogenic coronavirus-like agent (CVLA) and 6 known coronaviruses was examined by immunoelectron microscopy (IEM) and by immunofluorescence (IF). CVLA did not show cross reactivity with infectious bronchitis virus, transmissible gastroenteritis virus (TGEV),...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315166
更新日期：1981-01-01 00:00:00
abstract：:The 87,000-dalton glycoprotein (gp87) of bovine herpesvirus type 1 (BHV-1) was found to selectively attach to susceptible cells. The attachment of gp87 to the cells was markedly decreased by the prior adsorption of intact virions. Anti-gp87 (site Ia) monoclonal antibody, which inhibited BHV-1 adsorption to the cells a...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314428
更新日期：1987-01-01 00:00:00
abstract：:We investigated the frequency and the clinical relevance of hepatitis B virus (HBV) pre-S mutations in Qidong, China. The results showed HBV pre-S mutants were detected in 48.4% (47/97) of patients with HBV infection. Both pre-S deletion and pre-S2 start codon mutations were more frequently found in HCC than in CH pat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0176-9
更新日期：2008-01-01 00:00:00
abstract：:A small unconventional, viroid-like aetiological agent might initiate Crohn's disease (CD). Electrophoresis of radiolabelled low molecular weight nucleic acids from mesenteric lymph nodes and leucocytes did not distinguish CD-specific sequences compatible with a viroid-like agent. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310895
更新日期：1986-01-01 00:00:00
abstract：:The DNAs from nine Japanese field isolates of equine herpesvirus-1 (EHV-1) were analyzed by digestion with the restriction endonuclease Bam HI and Southern hybridization. Comparing restriction profiles among the EHV-1 strains, there was no considerable difference between isolates before and after vaccine application, ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01316881
更新日期：1993-01-01 00:00:00
abstract：:The V3 domain is highly variable and induces HIV neutralizing antibodies (NA). Here we addressed the issues of 1) the participation of mutations in V3 in generation of neutralization resistant escape virus in vivo and 2) the applicability of synthetic V3 peptides corresponding to field isolates to induce neutralizing ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309956
更新日期：1995-01-01 00:00:00
abstract：:Ninety four percent of the genome of bovine herpesvirus 4 (BHV-4) strain DN 599 was cloned and a physical map was constructed by Southern blot analysis using a library of cloned fragments cleaved with the 3 restriction enzymes (Eco RI, Bam HI, and Hin dIII). The genome length was estimated to be 156.5 kbp +/- 0.7. The...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309576
更新日期：1992-01-01 00:00:00
