Abstract:
:Next-generation sequencing of small RNAs and PCR amplification with two pairs of back-to-back primers, followed by cloning and sequencing, allowed identification of a novel geminivirus isolate provisionally named camellia chlorotic dwarf-associated virus (CaCDaV). The complete genome sequence of CaCDaV comprises 3,687 nucleotides (nts) and six open reading frames (ORFs). The genome and putative proteins of CaCDaV were most closely related to those of two unclassified geminiviruses: citrus chlorotic dwarf-associated virus (CCDaV, 3,640 nt) and mulberry mosaic dwarf-associated virus (MMDaV, 2,952 nt). Phylogenetic analysis revealed a distinct clade for CaCDaV, CCDaV and MMDaV, supporting their inclusion in a new genus within the family Geminiviridae. Because CaCDaV and CCDaV shared a high genome nucleotide sequence identity (54.8%), we propose that the CaCDaV should be included as a member in this new genus.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Zhang S,Shen P,Li M,Tian X,Zhou C,Cao Mdoi
10.1007/s00705-018-3780-3subject
Has Abstractpub_date
2018-06-01 00:00:00pages
1709-1712issue
6eissn
0304-8608issn
1432-8798pii
10.1007/s00705-018-3780-3journal_volume
163pub_type
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