Abstract:
:Respiratory syncytial virus (RSV) G glycoprotein mediates cell attachment through surface glycosaminoglycans (GAGs). Feldman et al. [10] suggested that specific basic amino acids in residues 184-198 of G defined a critical heparin binding domain (HBD). To further define the G HBD we made a series of truncated G proteins expressed in Escherichia coli. G88 (G residues 143-231), bound to HEp-2 cells in a dose dependent manner and binding was inhibited >99% with heparin. Cell binding of G88 was unaltered by alanine substitution mutagenesis of all basic amino acids in Feldman's region 184-198. A G88 variant truncated beyond residue 198, G58, and G58 fully alanine substituted in the region 184-198, G58A6, bound to HEp-2 cells about half as well and 100-fold less well than G88, respectively. G88 and all alanine substitution mutants of G88 inhibited RSV plaque formation by 50% (ID(50)) at concentrations of approximately 50 nM; the ID(50) of G58 was approximately 425 nM while G58A6 had an ID(50) >1600 nM. These data show that the G HBD includes as much as residues 187-231, that there is redundancy beyond the previously described HBD, and that the cell-binding and virus infectivity-blocking functions of these recombinant G proteins were closely linked and required at least one HBD.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Shields B,Mills J,Ghildyal R,Gooley P,Meanger Jdoi
10.1007/s00705-003-0139-0keywords:
subject
Has Abstractpub_date
2003-10-01 00:00:00pages
1987-2003issue
10eissn
0304-8608issn
1432-8798journal_volume
148pub_type
杂志文章abstract::Oral inoculation of entomopoxvirus spindles, microstructures composed of fusolin protein, causes disruption of the peritrophic matrix (PM), a physical barrier against microbe infection, in the insect midgut. Although the atomic structure of fusolin has been determined, little has been directly elucidated of the mechan...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-4013-5
更新日期:2019-01-01 00:00:00
abstract::Eleven cloned North American pseudorabies virus (PRV) strains and the European vaccine strains K and BUK were characterized by their thermal sensitivity and rabbit virulence markers. Heat sensitivity of the strains and isolates ranged from the highly heat resistant strain K, to the extremely heat labile strain BUK and...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01318093
更新日期:1979-01-01 00:00:00
abstract::Chronic hepatitis C virus (HCV) infection often leads to liver cancer. The HCV NS2 protein is a hydrophobic transmembrane protein that associates with several cellular proteins in mammalian cells. In this report, we investigated the function of NS2 protein on HCV replication and translation by using a transient cell-b...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0198-3
更新日期:2008-01-01 00:00:00
abstract::Begomovirus infection is becoming a threat in fresh-market tomato in Argentina, where mixed infections with begomoviruses are common. The complete sequence of a begomovirus isolate infecting tomato sampled in Salta was molecularly characterized. Phylogenetic analysis showed that this virus isolate is closely related t...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1355-2
更新日期:2012-10-01 00:00:00
abstract::In attempts to further develop murine leukemia virus (MLV) based retroviral vectors for gene therapy, we investigated vector production and antisense expression from retroviral constructs with U3 deletions or insertions. Promoter elements in the U3 region of the 3' LTR of the vector pLXSN were deleted and replaced wit...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050170029
更新日期:2001-01-01 00:00:00
abstract::Conditions for ELISA by using IgG linked with horseradish peroxidase have been developed for the detection of 6 arenaviruses (Machupo, Junin, Tacaribe, Amapari, LCM and Tamiami). This method allows the detection of arenavirus antigens in various materials; organs and blood in affected animals and infected cell culture...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314603
更新日期:1981-01-01 00:00:00
abstract::The complete genome sequence (9,865 nucleotides) of a highly divergent johnsongrass mosaic virus isolate (JGMV-CNPGL) was determined using Illumina sequencing. This isolate infected 10 genotypes of gramineous plants including maize. A comparative analysis of the complete genome showed 80 % nucleotide (nt) sequence ide...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2832-9
更新日期:2016-07-01 00:00:00
abstract::Senecavirus A (SVA) is associated with vesicular disease in swine and the acute death of neonatal piglets. Here, senecavirus A was isolated from a pig displaying vesicular disease in Northeast China. The virus was designated as SVA/HLJ/CHA/2016 and its full-length nucleotide sequence was determined and analyzed in com...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3480-4
更新日期:2017-10-01 00:00:00
abstract::The '6K1' protein of the Pinellia isolate of Soybean mosaic virus was cloned into a prokaryotic expression vector and a polyclonal antiserum raised to the expressed fusion protein. In immunogold labeling of thin sections of infected leaves of Pinellia ternata, specific labeling occurred at the cell periphery. This mig...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-007-0972-7
更新日期:2007-01-01 00:00:00
abstract::In this study, a novel mycovirus designed Colletotrichum gloeosporioides ourmia-like virus 1 (CgOLV1) was isolated from a filamentous phytopathogenic fungus, Colletotrichum gloeosporioides. The virus has a genome of 2,516 nucleotides and contains a large open reading frame (ORF) encoding an RNA-dependent RNA polymeras...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04346-2
更新日期:2019-10-01 00:00:00
abstract::Bovine leukemia virus (BLV) causes enzootic bovine leucosis (EBL) and is responsible for substantial economic losses in cattle globally. However, information in Africa on the disease is limited. Here, based on clinical, hematological, pathological and molecular analyses, two clinical cases of EBL were confirmed in a d...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-3205-0
更新日期:2017-04-01 00:00:00
abstract::A full-length cDNA clone of olive latent virus 1 (OLV-1), a member of the genus Necrovirus, family Tombusviridae, was subjected to site-directed mutagenesis, and coat protein gene mutants were constructed. A mutant clone, denoted Delta3297, was obtained by deleting the nucleotide in position 3297, thus inducing a fram...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-006-0767-2
更新日期:2006-10-01 00:00:00
abstract::Chilo iridescent virus (CIV; IIV-6) is the type member of the genus Iridovirus (family Iridoviridae, large icosahedral cytoplasmic DNA viruses). CIV induces death and deformity in the cotton boll weevil, Anthonomus grandis, replicates productively in larvae of the cotton boll weevil, and significantly reduces laborato...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-007-0945-x
更新日期:2007-01-01 00:00:00
abstract::RNA 3 of alfalfa mosaic virus (AMV) encodes the 5'-proximal movement protein (MP) gene and the 3'-proximal coat protein (CP) gene which is expressed from a subgenomic RNA. Several strategies were explored to use this RNA as a vector for expression of the green fluorescent protein (GFP) in Nicotiana tabaccum plants exp...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050170125
更新日期:2001-01-01 00:00:00
abstract::The E1 nucleic acid sequence of rubella virus strain Judith (RJ) has been cloned into an E. coli expression vector LB03. The reactivity of the expressed unglycosylated antigen (E1J) was compared with its glycosylated counterpart in native virus (RJ) using rabbit and human sera. Rabbit antisera raised against RJ and E1...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317201
更新日期:1992-01-01 00:00:00
abstract::Chikungunya fever is self-limiting. However, neurological and hemorrhagic complications have been seen in recent outbreaks. The clinical manifestations of this disease are similar to those of dengue virus infection, indicating the need for differential diagnosis in areas such as India, which are endemic for both virus...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2829-4
更新日期:2016-06-01 00:00:00
abstract::Six isolates of Cassava brown streak virus (CBSV, genus Ipomovirus; Potyviridae) from the Lake Victoria basin in Uganda and Tanzania were characterized. Virus particles were 650 nm long. The complete coat protein (CP)-encoding sequences (1,101 nucleotides, nt) were 90.7-99.5 and 93.7-99.5% identical at the nt and amin...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0301-9
更新日期:2009-01-01 00:00:00
abstract::Degenerate primers were used to detect and amplify 3'-terminal genome fragments of potyviruses from medicinal aroid plants growing at 16 sites in China. Virus was detected in 7 samples of which six, all of Pinellia ternata, contained a strain of soybean mosaic virus (SMV) similar to that previously reported from this ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0390-z
更新日期:2005-01-01 00:00:00
abstract::Vector transmission is an important part of the viral infection cycle, yet for many viruses little is known about this process, or how viral sequence variation affects transmission efficacy. Here we examined the effect of substituting genes from the highly transmissible FS577 isolate of citrus tristeza virus (CTV) in ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-3070-x
更新日期:2016-12-01 00:00:00
abstract::As the most common NK/T-cell lymphoma in Asian countries, extranodal NK/T-cell lymphoma, nasal type (ENKTL), has unique clinical features and a strong association with Epstein-Barr virus (EBV). In order to gain a preliminary understanding of the relationship between ENKTL and EBV, we performed genotypic analysis of EB...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2451-x
更新日期:2015-08-01 00:00:00
abstract::RNA extracted from 120 leaf specimens from 17 plant species was pooled, and polyadenylated RNA species were sequenced together without barcoding in one lane using massively parallel sequencing technology. After analysis, complete or partial genome sequences representing 20 virus isolates of 16 polyadenylated RNA speci...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-1166-x
更新日期:2012-02-01 00:00:00
abstract::Two contigs with high similarity to partitivirus sequences were identified by de novo assembly of sequences obtained by RNA-Seq from a wild brassicaceous plant, Arabidopsis halleri subsp. gemmifera. Here, we report the complete genome sequence of a putative novel partitivirus. Excluding the poly-A tail, it consists of...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04670-y
更新日期:2020-09-01 00:00:00
abstract::The presence of transmitted HIV drug resistance (TDR) threatens the efficacy of antiretroviral treatment. We aimed to assess the changes in TDR prevalence over the last decade in Madrid, Spain, to verify the role of the patients' risk groups in these changes. We analysed the trends of TDR between 2000 and 2011 in a co...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1933-y
更新日期:2014-05-01 00:00:00
abstract::The molecular epidemiology of a recent norovirus (NoV) outbreak in Brazil performed by comparative analysis with Genebank NoV sequences showed that the GII.4 strain was responsible for 72.5% of all NoV-positive cases (58/80). Other detected NoV strains included GII.3 (7/80; 8.8%) and GII.9 (8/80; 10%). This is the fir...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0078-x
更新日期:2008-01-01 00:00:00
abstract::We have developed an in vitro transcriptional assay using Escherichia coli RNA polymerase to initiate the replication of peach latent mosaic viroid (PLMVd). Regardless of the polarity of the PLMVd strand used as template, initiation in vitro occurred at the same hairpin structure. These initiation sites correspond to ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050170061
更新日期:2001-01-01 00:00:00
abstract::Toscana Virus, a sandfly-associated Phlebovirus, was adapted to grow in cultured Aedes pseudoscutellaris (AP-61) mosquito cell line. No evidence of virus growth was seen after primary infection of cell monolayers under maintenance conditions. On the contrary, persistent infections were established by subculturing infe...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317004
更新日期:1985-01-01 00:00:00
abstract::Noroviruses, a major cause of acute gastroenteritis outbreaks worldwide, are constantly evolving. This ability is reflected in the speed and efficiency with which these viruses spread and remain in the human population. The present study reports the detection of a novel recombination event among norovirus genotypes in...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1428-2
更新日期:2012-12-01 00:00:00
abstract::The envelope protein of human immunodeficiency virus type 1 (HIV-1) comprises the outer gp 120 SU domain and the anchoring gp41 TM domain, and the conventional view is that it has a single transmembrane region with the following C-terminal sequence situated entirely within the virion. However, we have recently propose...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050170199
更新日期:2001-01-01 00:00:00
abstract::In this study, bovine enteric caliciviruses (BECs) were detected in 49.4% of a total of 253 stool specimens for diarrheic calves collected from 42 industrial dairy farms from March 2010 to February 2012. Genogroup III norovirus (NoVsGIII) were more prevalent (39.5%) than neboviruses (NBs) (15%), and coinfections were ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3716-y
更新日期:2018-05-01 00:00:00
abstract::Modern adjuvants should induce strong and balanced immune responses, and it is often desirable to induce specific types of immunity. As an example, efficient Th1-immunity-inducing adjuvants are highly in demand. Such adjuvants promote good cell-mediated immunity against subunit vaccines that have low immunogenicity th...
journal_title:Archives of virology
pub_type: 杂志文章,评审
doi:10.1007/s00705-010-0863-1
更新日期:2011-02-01 00:00:00