Abstract：
:We have developed an in vitro transcriptional assay using Escherichia coli RNA polymerase to initiate the replication of peach latent mosaic viroid (PLMVd). Regardless of the polarity of the PLMVd strand used as template, initiation in vitro occurred at the same hairpin structure. These initiation sites correspond to the 5'-ends of two small (280 nt) PLMVd-related RNAs found in infected peach leaves. Using a series of truncated PLMVd-derived transcripts, we have demonstrated that the viroid domain composed solely of the self-complementary hammerhead sequences is sufficient to trigger polymerase-driven replication in vitro. These data suggest that the bacterial-like RNA polymerase from peach chloroplasts catalyzes PLMVd replication.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Pelchat M,Coté F,Perreault JPdoi
10.1007/s007050170061keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
1753-63issue
9eissn
0304-8608issn
1432-8798journal_volume
146pub_type
杂志文章abstract：:Entry processes were compared between dengue-2 (DEN-2) virus and a DEN-2 neutralisation escape mutant virus in baby hamster kidney (BHK) cells. The mutant virus (mu6B2) was resistant to neutralisation by a monoclonal antibody, MAb 6B2. Infection of BHK cells by the wild-type DEN-2 virus resulted in direct penetration ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050561
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journal_title：Archives of virology
pub_type： 杂志文章
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01316756
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pub_type： 杂志文章
doi：10.1007/s00705-002-0788-4
更新日期：2002-06-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309845
更新日期：1993-01-01 00:00:00
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journal_title：Archives of virology
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journal_title：Archives of virology
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doi：10.1007/s00705-011-1085-x
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317927
更新日期：1989-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
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更新日期：1994-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320619
更新日期：1980-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-0924-0
更新日期：2011-05-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718606
更新日期：1996-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0330-y
更新日期：2004-10-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313903
更新日期：1987-01-01 00:00:00
abstract：:Multidrug-resistant uropathogenic Escherichia coli (UPEC) is increasing gradually on a worldwide scale. We therefore examined the possibility of bacteriophage (phage) therapy for urinary tract infections (UTIs) caused by the UPEC strains as an alternative to chemotherapy. In addition to the well-known T4 phage, KEP10,...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0031-4
更新日期：2008-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0307-3
更新日期：2009-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309297
更新日期：1984-01-01 00:00:00
abstract：:Hemagglutinating and acetylesterase functions as well as the 124 kDa glycoprotein were present in the highly cell-culture adapted, avirulent bovine coronavirus strain BCV-L9, in the Norden vaccine strain derived from it, and in 5 wild-type, virulent strains that multiplied in HRT-18 cells but were restricted in severa...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309637
更新日期：1992-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF01309868
更新日期：1995-01-01 00:00:00
abstract：:Drug resistance mutations in the Pol gene of human immunodeficiency virus 1 (HIV-1) are one of the critical factors associated with antiretroviral therapy (ART) failure in HIV-1 patients. The issue of resistance to reverse transcriptase inhibitors (RTIs) in HIV infection has not been adequately addressed in the Indian...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2760-8
更新日期：2016-05-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311037
更新日期：1989-01-01 00:00:00
abstract：:Cucumber mosaic virus (CMV) is a three component isodiametric plant virus which is common worldwide and has an extremely wide host range. A pseudorecombinant was made, derived from the RNA3 component of the CMV-S strain, carrying the coat protein (CP) gene, and the RNA1,2 components of the CMV-D strain. This system de...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0190-x
更新日期：2004-01-01 00:00:00
abstract：:Hybridomas secreting monoclonal antibodies against herpes simplex virus (HSV) DNA binding proteins (DBP) have been produced. Five HSV DBP have been characterized according to molecular weight, affinity for DNA, kinetic class and localization within the infected cell. By preparing an immunoadsorbent column from antibod...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314300
更新日期：1984-01-01 00:00:00
abstract：:We focused on the role of membrane bound sugar residues in the infection of fibroblasts and monocyte-like cells with human cytomegalovirus (HCMV). Treatment of phorbol 12-myristate 13-acetate (PMA) differentiated monocyte-like cells THP-1 or human fibroblasts MRC-5 with lectins specific for N-acetylneuraminic acid (Ne...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322663
更新日期：1995-01-01 00:00:00
abstract：:RNA 3 of alfalfa mosaic virus (AMV) encodes the 5'-proximal movement protein (MP) gene and the 3'-proximal coat protein (CP) gene which is expressed from a subgenomic RNA. Several strategies were explored to use this RNA as a vector for expression of the green fluorescent protein (GFP) in Nicotiana tabaccum plants exp...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170125
更新日期：2001-01-01 00:00:00
abstract：:A group of previously isolated heterogeneous mEp lambdoid phages (43) from 19 different immunity groups for phage infection was further characterized to gain insight into some phenotypic traits and to assess their relationship with phage lambda. Interestingly, the FhuA host receptor was required by the majority of mEp...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0111-0
更新日期：2008-01-01 00:00:00
abstract：:We have sequenced the genome of a novel picornavirus, rabovirus A (rat-borne virus, RaBoV-A, NC_026314), which was present in the feces of a Norway rat (Rattus norvegicus) from Berlin, Germany. This virus is related to members of the genera Enterovirus and Sapelovirus. RaboV-A contains a type II IRES that is unlike th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2523-y
更新日期：2015-10-01 00:00:00
abstract：:A putative chrysovirus recovered from Brassica campestris var. purpurea and provisionally named "Brassica campestris chrysovirus 1" (BrcCV1) was sequenced. The genome of the putative BrcCV1 consists of three double-stranded RNAs (dsRNAs) comprising 3,639 (dsRNA 1), 3,567 (dsRNA 2) and 3,337 (dsRNA 3) base pairs, respe...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3196-x
更新日期：2017-04-01 00:00:00
abstract：:MicroRNAs (miRNAs) are a new class of well-conserved small noncoding RNAs that mediate posttranscriptional gene regulation. Hepatitis B virus (HBV) causes various liver diseases, including chronic hepatitis, liver cirrhosis and hepatocellular cancer. Recent data have indicated HBV alters miRNAs expression patterns, bu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2286-x
更新日期：2015-03-01 00:00:00
abstract：:The genus Bromovirus currently contains six species whose members have relatively narrow host ranges. In the present work, a new bromovirus infecting elderberry (Sambucus nigra L.) is reported. dsRNA was purified and sequenced by next-generation sequencing, and with minimal additional completion by Sanger sequencing, ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3629-1
更新日期：2018-02-01 00:00:00
